Co-administration of tilapia alpha-helical antimicrobial peptides with subunit antigens boost immunogenicity in mice and tilapia (Oreochromis niloticus)

Acosta, Jannel; Carpio, Yamila; Valdés, Iris; Velázquez, Janet; Zamora, Yasser; Morales, Reynold; Morales, Antonio; Rodríguez, Elsa; Estrada, Mario Pablo

doi:10.1016/j.vaccine.2013.11.009

Cited by 25 publications

(19 citation statements)

References 24 publications

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“…And since the other GBS serotypes were difficult to find, we only assessed GBS Ia, the principal serotype of infection in Nile tilapia [30]. Even though the immune systems of fish and higher vertebrates are similar, the immune system of fish produces antibodies at a slower rate than the immune system of higher vertebrates [31], so vaccines based on purified recombinant antigens induce a suboptimal immune response without the help of adjuvants and booster immunizations [32,33]. To make sure antibody production was at its best when the challenge was administered, a booster immunization and adjuvants (FIA and AH) were applied in our experiment.…”

Section: Discussionmentioning

confidence: 99%

A recombinant truncated surface immunogenic protein (tSip) plus adjuvant FIA confers active protection against Group B streptococcus infection in tilapia

Wang

Xiao³

et al. 2014

Vaccine

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Section: Discussionmentioning

confidence: 99%

A recombinant truncated surface immunogenic protein (tSip) plus adjuvant FIA confers active protection against Group B streptococcus infection in tilapia

Wang

Xiao³

et al. 2014

Vaccine

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“…Furthermore, combinations of Oreoch‐1, ‐2 and ‐3 co‐administered with my32‐Ls increased humoural and cellular responses to my32‐Ls in mice (Acosta et al . ). As their counterpart in higher vertebrates, zfBD2 stimulated genes coding for type I IFN, inflammatory cytokines, T‐cell markers, antigen‐presenting cell markers and granzymes are suggested as a potent viral DNA vaccine molecular adjuvant (García‐Valtanen et al .…”

Section: Potential Properties and Applications Of Fish Antimicrobial mentioning

confidence: 97%

“…Given that fish AMPs show many different properties apart from their antimicrobial function, they are suggested as potential adjuvants to improve vaccines. Hence, it was demonstrated that tilapia piscidin-like peptides are able to boost immune responses in both mammals and fish via type 1 immune response (Th1) molecular pathways (Acosta et al 2014). On the one hand, the co-administration of Oreoch-1 with sea lice my32 protein (which affects the expression of signal transduction and innate immune-related genes) from the copepod Lepeophtheirus salmonis (my32-Ls) successfully enhanced humoural immunity in mice and tilapia.…”

Section: Amps As Vaccine Adjuvantsmentioning

confidence: 99%

“…On the other hand, when Oreoch-1 was co-administered with ovalbumin, it was able to generate a Th1 humoural immune response in mice, whereas Oreoch-2 and -3 triggered a Th1 cellular response characterized by the induction of IFNc in a dose-dependent manner. Furthermore, combinations of Oreoch-1, -2 and -3 co-administered with my32-Ls increased humoural and cellular responses to my32-Ls in mice (Acosta et al 2014). As their counterpart in higher vertebrates, zfBD2 stimulated genes coding for type I IFN, inflammatory cytokines, T-cell markers, antigen-presenting cell markers and granzymes are suggested as a potent viral DNA vaccine molecular adjuvant (Garc ıa-Valtanen et al…”

Section: Amps As Vaccine Adjuvantsmentioning

confidence: 99%

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Antimicrobial peptides from fish: beyond the fight against pathogens

Valero

Saraiva‐Fraga

Costas

et al. 2018

Reviews in Aquaculture

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Fish antimicrobial peptides (AMPs) are small peptides whose actions have great implications in the innate immune response of teleost fish, being considered critical as the first line of defence against a wide spectrum of pathogens. Antibiotics have been widely used in aquaculture, resulting in bacterial resistance. The potential of AMPs as alternative to antibiotics makes them a very interesting tool as they represent lower‐risk peptides to develop resistances, or even in the fight against other pathogens such as viruses. Beyond their antimicrobial function, fish AMPs possess several relevant but little‐known characteristics and capabilities such as host‐defence peptides, antioxidants, active compounds of immunogenic drugs and as adjuvants. Moreover, their antitumour properties make them potential drugs to be used in oncological treatments in human subjects. Novel methodologies as clustered regularly interspaced short palindromic repeats associated proteins system (CRISPR‐Cas), or even their use as nutraceutics, seem to be promising instruments to enlarge the functions of fish AMPs. This review discusses these new methodologies, as well as the latest research on fish AMP functions, newly discovered properties of AMPs and their future applications in both the aquaculture industry and human health care.

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“…12 Furthermore, they serve as adjuvants in fish vaccination. 13 Tilapia piscidin 3 (TP3) is a 23 amino acid peptide, first isolated from Nile tilapia (Oreochromis niloticus) since 2012, which starts with phenylalanine and ends with histidine. TP3 has antimicrobial activity against both Gram-positive and Gram-negative bacteria.…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization and immunohistochemical localization of tilapia piscidin 3 in response to Aeromonas hydrophila infection in Nile tilapia

Mahrous

Mabrouk

Aboelenin

et al. 2020

Journal of Peptide Science

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The antimicrobial activity of tilapia piscidin 3 (TP3) was determined in vitro against a locally isolated Aeromonas hydrophila. A 388 BP fragment was amplified from the TP3 cDNA and sequenced. The coding sequence (CDS) of TP3 was estimated to be 231 BP codes for 76 amino acids long and stop codon. In silico analysis was performed to detect both the signal peptide and the prodomain cleavage sites to follow the amino acids number 22 and 70, respectively. Based on this, a peptide 23 amino acids long with a remarkably high computed antimicrobial probability was synthesized and used in the subsequent experiments. The antimicrobial activity of TP3 was determined with minimum inhibitory concentration (MIC) and minim um bactericidal concentration (MBC) methods. TP3 exhibited relatively weak antimicrobial activities against the tested bacteria. A challenge experiment was then performed in Nile tilapia with low and high doses of A. hydrophila, followed by timely recognition; after 3, 6, 24 h, and 7 days of the specific TP3 gene expression, immunohistochemical localization was also performed. Histopathological examination revealed provoked inflammatory responses and congestion in the same organs of TP3 expression. Immunohistochemical localization showed that A. hydrophila induced tilapia fish to express TP3 after 24 h within the gills, intestine, hepatopancreas, spleen, and posterior kidney. In quantitative real time (RT)-polymerase chain reaction analysis, the high dose showed higher mRNA expression levels than the low dose, and its expression levels increased in the A. hydrophila-infected fish. It was therefore concluded that TP3 plays an essential role in fish immunity.

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Co-administration of tilapia alpha-helical antimicrobial peptides with subunit antigens boost immunogenicity in mice and tilapia (Oreochromis niloticus)

Cited by 25 publications

References 24 publications

A recombinant truncated surface immunogenic protein (tSip) plus adjuvant FIA confers active protection against Group B streptococcus infection in tilapia

A recombinant truncated surface immunogenic protein (tSip) plus adjuvant FIA confers active protection against Group B streptococcus infection in tilapia

Antimicrobial peptides from fish: beyond the fight against pathogens

Molecular characterization and immunohistochemical localization of tilapia piscidin 3 in response to Aeromonas hydrophila infection in Nile tilapia

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