Co-administration of rotavirus nanospheres VP6 and NSP4 proteins enhanced the anti-NSP4 humoral responses in immunized mice

Afchangi, Atefeh; Jalilvand, Somayeh; Arashkia, Arash; Latifi, Tayebeh; Farahmand, Mohammad; Shirazi, Maryam Mashhadi Abolghasem; Nasab, Seyed Dawood Mousavi; Marashi, Sayed Mahdi; Roohvand, Farzin; Shoja, Zabihollah

doi:10.1016/j.micpath.2022.105405

Cited by 6 publications

(2 citation statements)

References 79 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Notably, a large portion of the puri ed protein was lost during the washing process which was obvious on SDS-PAGE by the presence of VP6 protein in the wash fraction. Similar phenomena were reported by Afchangi et al [70], who observed partial loss of VP6 protein trimer structures in western blot assays. This indicates the need to use different puri cation method, like ion exchange or gel ltration, to minimize the loss of the protein during the puri cation process.…”

Section: Discussionsupporting

confidence: 89%

Neutralization and immunoperoxidase to estimate the immunogenicity of recombinant human rotavirus VP6 structural protein in vitro after insertion of the antibodies into cells using electroporation

Kamel,

Shokeer,

Hegazy

et al. 2024

Preprint

View full text Add to dashboard Cite

Rotaviruses (RVs) represent the principal causative agent of severe gastroenteritis leading to high mortality rates, especially in children < 5 years in both developed and developing countries. Although, the first-generation of live attenuated RV vaccines such as RotaTeq and Rotarix achieved partial success in reducing the number of RV deaths worldwide, several concerns, such as low efficacy especially in developing countries, safety, and cost imply a dire need to develop these vaccines. Also, sensitive methods to estimate the immunogenicity of the candidate recombinant subunit VP6 vaccines in vitro are of great need. In the present study, 1232 bp of the most frequent full length VP6 in clinical and environmental isolates in Egypt with 98% nucleotides identity and 98% amino acid identity in comparison to human RoV Wa reference strain was expressed in E.coli. The examination of the sensitivity of the antibodies produced in the male rabbits which were immunized intramuscularly with 20 µg of the purified VP6 proteins, indicated a sensitivity up to 1/24000 dilution of antibodies against the expressed protein using ELISA. Introduce antibodies into MA104 cell line was performed using electroporation to neutralize the human rotavirus Wa strain VP6 when exposed after viral uncoating. Higher sensitivity of neutralization in relation to immunoperoxidase was observed for the estimation of the antibodies which act intracellularly against high and low infectious units of human rotavirus Wa strain in vitro. Promising sensitivity of the produced antibodies against the infectious human RV Wa strain was observed.

show abstract

Section: Discussionsupporting

confidence: 89%

Neutralization and immunoperoxidase to estimate the immunogenicity of recombinant human rotavirus VP6 structural protein in vitro after insertion of the antibodies into cells using electroporation

Kamel,

Shokeer,

Hegazy

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…PoRV is generated through a three-layer protein capsid, with the middle layer of the capsid consisting mainly of the VP6 protein (Suzuki et al, 2014).The VP6 protein is the core structural protein of the viral particles, and is therefore often used as a target protein for RV detection (Shoja et al, 2022). Previous ndings have shown that the VP6 protein is the basis of PoRV transcriptase and replicase, and is able to initiate the transcriptionally active protein trimerization structure, which is important for viral replication and protein translation (Afchangi et al, 2022). In addition, VP6 is a potent antigen that stimulates the body to produce speci c immune responses.. Lactobacillus (LAB) is a probiotic that exerts great advantages in the prevention of diarrhea and immune enhancement (Reuben et al, 2019, De Filippis et al, 2020.…”

Section: Introductionmentioning

confidence: 99%

L. plantarum surface-displayed VP6 (PoRV) protein can prevent PoRV infection in piglets

Wang,

Zhang

et al. 2023

Preprint

View full text Add to dashboard Cite

Porcine rotavirus (PoRV) poses a threat to the development of animal husbandry and human health, leading to substantial economic losses. Among its components, VP6 protein constitutes a major portion of the viral particles and serves as the core structural protein of the virus. In this study, we first developed an antibiotics free and environmentally friendly vector called asd-araC-PBAD-alr (AAPA). Subsequently, we constructed recombinant Lactobacillus plantarum (L. plantarum) strains induced by arabinose to express VP6 and VP6-pFc fusion proteins. Our findings revealed that NC8/Δalr-pCXa-VP6-S and NC8/Δalr-pCXa-VP6-pFc-S strains were capable of enhancing body immunity and protecting suckling mice and piglets from rotavirus infection. The novel recombinant L. plantarum strains developed in this study hold promising potential as oral vaccines to enhance body immunity and present a new strategy for preventing PoRV infection.

show abstract

Correlates of immune protection against human rotaviruses: natural infection and vaccination

Latifi,

Kachooei,

Jalilvand

et al. 2024

Arch Virol

View full text Add to dashboard Cite

Co-administration of rotavirus nanospheres VP6 and NSP4 proteins enhanced the anti-NSP4 humoral responses in immunized mice

Cited by 6 publications

References 79 publications

Neutralization and immunoperoxidase to estimate the immunogenicity of recombinant human rotavirus VP6 structural protein in vitro after insertion of the antibodies into cells using electroporation

Neutralization and immunoperoxidase to estimate the immunogenicity of recombinant human rotavirus VP6 structural protein in vitro after insertion of the antibodies into cells using electroporation

L. plantarum surface-displayed VP6 (PoRV) protein can prevent PoRV infection in piglets

Correlates of immune protection against human rotaviruses: natural infection and vaccination

Contact Info

Product

Resources

About