CNOT6/6L-mediated mRNA degradation in ovarian granulosa cells is a key mechanism of gonadotropin-triggered follicle development

Dai, Xing‐Xing; Jiang, Zhi-Yan; Wu, Yun-Wen; Sha, Qian‐Qian; Liu, Yang; Ding, Jian; Xi, Wen-Dong; Li, Jing; Fan, Heng‐Yu

doi:10.1016/j.celrep.2021.110007

Cited by 15 publications

(8 citation statements)

References 45 publications

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“…The miRNAs promote the degrading and inhibiting translation of mRNA by interacting with their 5′ and 3′ untranslated region (UTR) sequences. Our results showed that TGFBR1 [ 30 ], SUPT6H [ 31 ], CNOT6L [ 32 ], and BTBD10 [ 33 ], which were reported to participate in the regulation of cell apoptosis and follicular development, may be the key target genes for miR-202-5p to regulate goose GCs. Subsequently, the result of qRT-PCR indicates that miR-202-5p impacted the expression of BTBD10 most significantly.…”

Section: Discussionmentioning

confidence: 99%

MiR-202-5p Regulates Geese Follicular Selection by Targeting BTBD10 to Regulate Granulosa Cell Proliferation and Apoptosis

Ran

Xie

et al. 2023

IJMS

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The regulation of granulosa cells (GCs) proliferation and apoptosis is the key step in follicular selection which determines the egg production performance of poultry. miR-202-5p has been reported to be involved in regulating the proliferation and apoptosis of mammalian ovarian GCs. However, its role in regulating the proliferation and apoptosis of goose GCs is still unknown. In the present study, the GCs of pre-hierarchical follicles (phGCs, 8–10 mm) and those of hierarchical follicles (hGCs, F2–F4) were used to investigate the role of miR-202-5p in cell proliferation and apoptosis during follicle selection. In phGCs and hGCs cultured in vitro, miR-202-5p was found to negatively regulate cell proliferation and positively regulate cell apoptosis. The results of RNA-seq showed that BTB Domain Containing 10 (BTBD10) is predicted to be a key target gene for miR-202-5p to regulate the proliferation and apoptosis of GCs. Furthermore, it is confirmed that miR-202-5p can inhibit BTBD10 expression by targeting its 3′UTR region, and BTBD10 was revealed to promote the proliferation and inhibit the apoptosis of phGCs and hGCs. Additionally, co-transfection with BTBD10 effectively prevented miR-202-5p mimic-induced cell apoptosis and the inhibition of cell proliferation. Meanwhile, miR-202-5p also remarkably inhibited the expression of Phosphatidylinositol-4,5-Bisphosphate 3-Kinase Catalytic Subunit Beta (PIK3CB) and AKT Serine/Threonine Kinase 1 (AKT1), while it was significantly restored by BTBD10. Overall, miR-202-5p suppresses the proliferation and promotes the apoptosis of GCs through the downregulation of PIK3CB/AKT1 signaling by targeting BTBD10 during follicular selection. Our study provides a theoretical reference for understanding the molecular mechanism of goose follicular selection, as well as a candidate gene for molecular marker-assisted breeding to improve the geese’ egg production performance.

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Section: Discussionmentioning

confidence: 99%

MiR-202-5p Regulates Geese Follicular Selection by Targeting BTBD10 to Regulate Granulosa Cell Proliferation and Apoptosis

Ran

Xie

et al. 2023

IJMS

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“…CNOT6/6L-mediated mRNA deadenylation is essential for female reproductive endocrine regulation. FSH stimulates the transcription and translation of Cnot6 and Cnot6l in ovarian GCs, which could function as key effectors of FSH in GCs and trigger the clearance of specific transcripts in GCs during the preantral-to-antral follicle transition ( Dai et al., 2021 ). In this study, we found that FSH could also induce Cnot6 and Cnot6l mRNA expression in mGCs, with a higher level in GCs from the Shp2 gcko mice.…”

Section: Discussionmentioning

confidence: 99%

“…During folliculogenesis, FSH specifically activates the PI3K/AKT pathway and its target proteins, resulting in follicular growth and maturation ( Hunzicker-Dunn et al., 2012 ; Li et al., 2013 ). FSH stimulates the transcription and translation of Cnot6 and Cnot6l in GCs, which could function as key effectors of FSH in GCs and trigger the clearance of specific transcripts in GCs during the preantral-to-antral follicle transition ( Dai et al., 2021 ). Hydrogen peroxide (H 2 O 2 ) effectively offsets FSH-evoked GC growth by inhibiting PI3K/AKT activation, which involves the initiation of GC death and follicular atresia ( Hanukoglu, 2006 ).…”

Section: Introductionmentioning

confidence: 99%

Tyrosine phosphatase SHP2 in ovarian granulosa cells balances follicular development by inhibiting PI3K/AKT signaling

Wei

Zheng

Ying

et al. 2022

Journal of Molecular Cell Biology

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In mammals, the growth and maturation of oocytes within growing follicles largely depends on ovarian granulosa cells (GCs) in response to gonadotropin stimulation. Many signals have been shown to regulate GC proliferation and apoptosis. However, whether the tyrosine phosphatase SHP2 is involved remains unclear. In this study, we identified the crucial roles of SHP2 in modulating GC proliferation and apoptosis. The production of both mature oocytes and pups was increased in mice with Shp2 specifically deleted in ovarian GCs via Fshr-Cre. Shp2 deletion simultaneously promoted GC proliferation and inhibited GC apoptosis. Furthermore, Shp2 deficiency promoted, while Shp2 overexpression inhibited, the proliferation of cultured primary mouse ovarian GCs and the human ovarian granulosa-like tumor cell line KGN in vitro. Shp2 deficiency promoted follicule-stimulating hormone (FSH)-activated phosphorylation of AKT in vivo. SHP2 deficiency reversed the inhibitory effect of hydrogen peroxide (H2O2) on AKT activation in KGN cells. H2O2 treatment promoted the interaction between SHP2 and the p85 subunit of PI3K in KGN cells. Therefore, SHP2 in GCs may act as a negative modulator to balance follicular development by suppressing PI3K/AKT signaling. The novel function of SHP2 in modulating proliferation and apoptosis of GCs provides a potential therapeutic target for the clinical treatment of follicle developmental dysfunction.

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“…In addition, super-stimulation of ovarian follicles via gonadotropin injections resulted in changes in small, medium, and large follicles according to the number and dose of gonadotropin injections and side of the ovary (Abdelnaby et al, 2021). FSH stimulates transcription and translation in ovarian granulosa cells, which are essential for female reproductive endocrine regulation (Dai et al, 2021). Collectively, it could be concluded that the in vivo and in vitro conditions or factors effect on oocytes' maturation and their developmental competence to embryos and fetus.…”

Section: Factors Affecting Oocyte Maturationmentioning

confidence: 99%

Changes in Nucleus and Cytoplast During Oocyte Maturation: Involvement in Embryo Production

Zeidi¹,

Masruri²,

Mufarji³

et al. 2022

AAVS

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Embryo production steps either in vivo or in vitro include oocyte meiotic maturation, fertilization and culture processes. Oocyte maturation is the first step for in vivo or in vitro embryo production. Oocyte maturation appears to be a simple step, but it is a very complex process. Maturation of oocytes include several changes at the cytoplasmic and nuclear levels that makes the oocytes able to undergo fertilization, pre-implantation and post-implantation embryo development. Oocyte meiotic maturation in vivo starts after LH hormone surge whereas it starts in vitro upon release of oocyte from the ovarian follicle. Rodent oocyte maturation lasts 15-17hr. whereas that of ruminant oocyte lasts 24hr. Several factors affect on oocytes maturation either in vitro or in vivo and therefore affect development of the resulting embryos. Because of the significance of oocyte maturation stage on further embryonic development, this review aims to discuss the knowledge of oocyte maturation and its effect on further development of embryos in vivo and in vitro.

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CNOT6/6L-mediated mRNA degradation in ovarian granulosa cells is a key mechanism of gonadotropin-triggered follicle development

Cited by 15 publications

References 45 publications

MiR-202-5p Regulates Geese Follicular Selection by Targeting BTBD10 to Regulate Granulosa Cell Proliferation and Apoptosis

MiR-202-5p Regulates Geese Follicular Selection by Targeting BTBD10 to Regulate Granulosa Cell Proliferation and Apoptosis

Tyrosine phosphatase SHP2 in ovarian granulosa cells balances follicular development by inhibiting PI3K/AKT signaling

Changes in Nucleus and Cytoplast During Oocyte Maturation: Involvement in Embryo Production

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