Cnidicin, a Coumarin, from the Root of Angelica koreana, Inhibits the Degranulation of Mast Cell and the NO Generation in RAW 264.7 Cells

Abstract

Cnidicin (1) and five related coumarins were isolated from the root extract of Angelica koreana (Umbelliferae) as active principles responsible for the inhibitory effect on the degranulation process of cultured mast cells. Cnidicin (1) demonstrated a significant inhibition upon the release of beta-hexosaminidase from the cultured RBL-2H3 cells in a dose dependent manner (IC50 value, 25 microM) and upon the nitric oxide production from the activated RAW264.7 cells (IC50 value, 7.5 microM). In agreement with this, cnidicin inhibited the expression of nitric oxide synthase in RAW264.7 cells.

Antiproliferative Effect of Furanocoumarins from the Root of Angelica dahurica on Cultured Human Tumor Cell Lines

“…6. Each compound was identified by direct comparison of spectral properties, MS, 1 H-NMR and 13 C-NMR with those of the authentic compounds in the literature (Ryu et al, 2001). tumor cell lines, i.e.…”

Coumarin and Flavone Derivatives from Estragon and Thyme as Inhibitors of Chemical Mediator Release from RBL-2H3 Cells

“…Coumarin derivatives occur in various spice plants, and have been reported to have antitumoral, 15) fungicidal 16) and anti-inflammatory 17) activities. Although Ryu et al 18) have reported that cnidicin, a coumarin derivative, inhibited mast cell degranulation, degranulation inhibiting activity of 7-methoxycoumarin has not previously been reported.…”

Agents Protecting against Sepsis from the Roots of Angelica dahurica

“…Their structures were identified by comparison of optical rotations, 1 H-and 13 C-NMR, DEPT, and MS data with the reported ones of the corresponding compounds. 13,[17][18][19][20][21] Among the purified compounds, 3 showed the strongest protective effect against lethality induced by LPS/D-GalN ( Table 2). Pretreatment of mice with 3 at doses of 3, 10, and 30 mg/kg increased survival rates to 40%, 80%, and 100%, respectively, compared with 20% in the control experiment, while pretreatment with dexamethasone as a positive control at doses of 3, 10, and 30 mg/kg increased survival rates to 60%, 100%, and 100%, respectively.…”

“…10 (1.97 g), and Fr. 11 (3 g 17,20,21) respectively. The structures of the four compounds are shown in Fig.…”