CNF producing Escherichia coli isolated from cattle in Northern Ireland

Burns, A.L.; Ball, H.J.; Finlay, D.

doi:10.1016/0378-1135(95)00196-4

Cited by 18 publications

(20 citation statements)

References 19 publications

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“…All of the NTEC‐positive animals were infected with CNF2‐producing E. coli . This is in agreement with the results of other authors (Blanco et al., 1992b, 1993, 1998; Burns et al., 1996; Orden et al., 1999) who found that CNF2‐positive NTEC strains were frequently isolated from healthy calves. These results suggest that NTEC producing CNF2 may be a part of the normal intestinal flora of cattle.…”

Section: Discussionsupporting

confidence: 93%

Characterization of NecrotoxigenicEscherichia coli(NTEC) Strains Isolated from Healthy Calves in Poland

Osek¹

2001

Journal of Veterinary Medicine, Series B

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Faecal samples from 132 healthy, 4±8-week-old calves from four different farms were examined for necrotoxigenic Escherichia coli (NTEC) producing the cytotoxic necrotizing factors type 1 (CNF1) and type 2 (CNF2). CNF2 genes were detected by polymerase chain reaction in 24 (6.1%) of the 396 E. coli strains tested; these strains were found in 18 (13.6%) calves used in the study. None of the 396 E. coli isolates examined possessed the gene encoding CNF1. Overall, 28.8% of E. coli examined expressed the F17 ®mbrial antigen. A strong association between CNF2 toxin and F17 ®mbriae was found (62.5% of CNF2-positive strains were F17-positive). Moreover, six out of 24 NTEC strains had the Stx1 or the Stx2 shiga toxin genes, and three additional isolates possessed the eae genetic marker of the intimin protein.

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Section: Discussionsupporting

confidence: 93%

Characterization of NecrotoxigenicEscherichia coli(NTEC) Strains Isolated from Healthy Calves in Poland

Osek¹

2001

Journal of Veterinary Medicine, Series B

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“…Nevertheless, the proportions of NTEC strains reported in more recent studies (Burns et al, 1996;Pohl et al, 1997;reviewed in De Rycke et al, 1999) are much higher. These differences can be explained by (a) the type of the animal populations sampled as for instance the risk of NTEC infection increases after the ®rst month of life (Orden et al, 1999); or (b) the changes throughout the years in animal husbandry as intensive rearing, which can favour the dissemination of bacterial pathogens, has increased since the early 1960s; or (c) the decrease of incidence of other pathogens because of husbandry and vaccinal prophylaxis as for enterotoxigenic E. coli; or (d) the selection of resistant clones and/or plasmids by extensive antibiotic use; or (e) an increased awareness and the apparition of better laboratory techniques.…”

Section: Discussionmentioning

confidence: 91%

“…The O serogroups recognised vary, but the most frequently observed were O78 and O15; which are indeed frequently associated with bovine NTEC2 strains (Burns et al, 1996;Mainil et al, 1999). On the other hand, serogroups observed among NTEC1 are not common Mainil et al, 1999).…”

Section: Discussionmentioning

confidence: 98%

Characteristics of necrotoxigenic Escherichia coli isolated from septicemic and diarrheic calves between 1958 and 1970

Bost

Bâbe

Jacquemin

et al. 2001

Veterinary Microbiology

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A total of 434 Escherichia coli isolated from septicemic calves between 1958 and 1965 and 430 E. coli isolated from diarrheic calves between 1967 and 1970 were studied by colony hybridisation and PCR assays for the presence of the cnf1-and the cnf2-like genes. They were also studied for the presence of genes coding for putative virulence factors associated with the CNF toxins including F17-, Pap-and Sfa-®mbrial adhesins and the recently described CDT-III toxin and AfaVIII-a®mbrial adhesin. Thirty (7%) of the 434 septicemic strains were positive for CNF by colony hybridisation. Twenty-six were con®rmed as necrotoxigenic E. coli type 2 (NTEC2) and four as NTEC1 by PCR. Thirty-®ve (8%) of the 430 diarrheic strains were positive for CNF by colony hybridisation. Five of them were studied by PCR and con®rmed as NTEC1. The 26 septicemic NTEC2 strains and 20 of the 35 diarrheic NTEC including three of the ®ve NTEC1 were positive for CDT-III. All adhesins studied were present in NTEC as well as in non-NTEC. NTEC1 were mainly Pap-, Sfa-and/or Afa8-positive, whereas NTEC2 were mainly F17-and/or Afa8-positive. This study shows that necrotoxigenic E. coli with their associated adhesins and toxins were present in calves as early as 1958, but their prevalence seems to have increased since that time. #

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“…Several groups have reported an epidemiological link between the presence of cnf1+ genotype, or the production of CNF1, and E. coli strains that caused extraintestinal disease. CNF2, is a commonly occurring virulence factor among healthy cattle (Burns, Ball, & Finlay, 1996;Pohl et al, 1993). Indeed, we reported a high prevalence of the CNF1 in faeces of healthy cattle (Hassan & Awad-Masalmeh -unpublished data).…”

Section: Discussionmentioning

confidence: 98%

Bacterial quality of raw milk investigated by Escherichia coli and isolates analysis for specific virulence-gene markers

Altalhi

Hassan

2009

Food Control

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CNF producing Escherichia coli isolated from cattle in Northern Ireland

Cited by 18 publications

References 19 publications

Characterization of NecrotoxigenicEscherichia coli(NTEC) Strains Isolated from Healthy Calves in Poland

Characterization of NecrotoxigenicEscherichia coli(NTEC) Strains Isolated from Healthy Calves in Poland

Characteristics of necrotoxigenic Escherichia coli isolated from septicemic and diarrheic calves between 1958 and 1970

Bacterial quality of raw milk investigated by Escherichia coli and isolates analysis for specific virulence-gene markers

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