CMV gB genotypes and outcome of vertical transmission: study on dried blood spots of congenitally infected babies

Barbi, Maria; Binda, Sandro; Caroppo, Simona; Prímache, V.; Didò, P.; Guidotti, Paola; Corbetta, Carlo; Melotti, D.

doi:10.1016/s1386-6532(00)00188-8

Cited by 81 publications

(80 citation statements)

References 13 publications

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“…Of particular interest in the present study with a homogeneous demographic population, we found that the HCMV gB genotypes are distributed differently among subgingival samples from patients with different periodontal status. Four types of HCMV gB genes are known (3,4), but only two types were detected in subgingival samples from the Chinese individuals. To determine if there are other types of HCMV gB genes in subigingival plaque samples, we expanded our research by recruiting more subjects.…”

Section: Discussionmentioning

confidence: 99%

Correlation between Infections with Different Genotypes of Human Cytomegalovirus and Epstein-Barr Virus in Subgingival Samples and Periodontal Status of Patients

Wu¹,

Yan

Ojcius

et al. 2007

J Clin Microbiol

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Accumulating evidence indicates that herpesviruses may be putative pathogens in various types of periodontal diseases. The present study was performed to examine infections with different genotypes of human cytomegalovirus (HCMV) and Epstein-Barr virus (EBV) in subgingival samples from a Chinese population and to analyze the correlation with periodontal status. A nested PCR assay was used to identify the presence of HCMV, EBV type 1 (EBV-1), and EBV-2; and the amplicons were further analyzed by restriction fragment length polymorphism analysis. HCMV was detected in 79.0% of 143 chronic periodontitis (CP) patients, 78.5% of 65 gingivitis patients, and 76.3% of 76 periodontally healthy individuals, while EBV was found in 63.6%, 32.3%, and 30.3% of the three groups of subjects, respectively. The HCMV-positive PCR products from all the samples were identified as corresponding to gB genotype I (gB-I) or gB-II. HCMV gB-II (62.9%), EBV-1 (43.4%), and EBV-2 (18.2%) were associated with CP at higher frequencies (P < 0.05), whereas HCMV gB-I was more often observed in gingivitis patients (40.0%) and healthy individuals (40.8%) (P < 0.05). Furthermore, a higher rate of coinfection with HCMV and EBV was shown in CP patients (52.4%), especially dual infections with HCMV gB-II and EBV-1 (30.8%) or HCMV gB-II and EBV-2 (12.6%), compared with the rates of single infections with HCMV or EBV (P < 0.05). Infection with HCMV gB-II, EBV-1, or EBV-2 was correlated with higher rates of bleeding on probing (P < 0.05). In patients infected with HCMV gB-II or both HCMV and EBV, including HCMV gB-II and EBV-1, a deeper probing depth or more serious attachment loss was found (P < 0.05). These findings clearly indicate that HCMV gB-II is the dominant genotype detected in subgingival samples in CP. HCMV gB-II infection and HCMV gB-II coinfection with EBV-1 are closely associated with periodontal tissue inflammation and destruction.

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Section: Discussionmentioning

confidence: 99%

Correlation between Infections with Different Genotypes of Human Cytomegalovirus and Epstein-Barr Virus in Subgingival Samples and Periodontal Status of Patients

Wu¹,

Yan

Ojcius

et al. 2007

J Clin Microbiol

View full text Add to dashboard Cite

show abstract

“…These results differ from those of studies undertaken in other parts of the world. For example, a study in the United States and another in Italy showed that the most frequent genotype was gB1, followed (in order) by gB3, gB2, and gB4 [35,36]. In a population in China, gB1 was the most frequent genotype, followed bygB2 and gB3 (which did not differ significantly), and more mixed infections were also observed [37].…”

Section: Discussionmentioning

confidence: 99%

Detection and gB genotyping of CMV in Mexican preterm infants in the context of maternal seropositivity

Arellano‐Galindo

Villanueva-García

Cruz-Ramirez

et al. 2014

J Infect Dev Ctries

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Introduction: Congenital (CI) and perinatal cytomegalovirus (CMV) infections (PI) can be linked to maternal CMV seropositivity, with fatal consequences in preterm newborns. GB genotyping has been used to analyze genotypic similarity in mothers and infants. The frequency of CMV infection in the context of maternal seropositivity and the viral gB genotypes as well as the genotypic similarity in mothers and preterm infants were investigated. Methodology: Saliva samples and dry blood spots (DBS) were taken weekly from preterm newborns from birth until the first month of life, and breast milk samples were taken from their mothers weekly during the first month of lactation. CMV IgG seroprevalence of the mothers and CI or PI in the infants were established. The gB status and genotypic similarities were established retrospectively in DBS and in the breast milk samples. Results: In total, 387 neonates and 375 mothers were enrolled. The maternal CMV-positive IgG serology was 97.3% (365/375). Neonatal CMV was found in 5.1% (20/387) of newborns, and one infant presented with CMV-compatible symptoms. CI was 2.5% and PI in the first month after birth was 11.8%. GB2 was the most prevalent genotype and was also the genotype preferentially transmitted to newborns by mothers with mixed infections. Conclusions: CMV PI and CI in preterm infants from highly seropositive mothers was high, but the rate of symptomatic infection was low. The prevalent genotype was gB2, and this genotype was preferentially transmitted to newborns by mothers with mixed infections.

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“…It is therefore not relevant to test for UL144 genotypes in this context. Other HCMV polymorphisms, such as glycoprotein B genotypes, have been evaluated in congenital infection and also proved to be disappointing (2,3,5,13,15,21). Moreover, it was recently demonstrated that the inter-and intragenic variability of HCMV clinical isolates leads to an infinite number of genetic combinations, probably explaining the failure to use sequence information to predict disease outcome (16).…”

Section: Discussionmentioning

confidence: 99%

Human Cytomegalovirus UL144 Gene Polymorphisms in Congenital Infections

Picone

Costa

Chaix³

et al. 2005

J Clin Microbiol

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The human cytomegalovirus (HCMV) UL144 gene is a tumor necrosis factor-like receptor with the potential to affect HCMV virulence. HCMV strains display genetic variability in the UL144 region, and the analysis of a potential link between UL144 gene polymorphisms and disease severity has scarcely been studied. However, a correlation between the UL144 genotype and congenital-disease outcome has been reported in one previous study, with the observation that all asymptomatic infants had a single UL144 genotype. In order to confirm or refute this finding, we determined the UL144 polymorphisms of HCMV strains recovered from the amniotic fluids of 38 infected fetuses and compared them to HCMV strains obtained from 30 viremic adult controls. The UL144 sequences were distributed among five genotypes (A, B, C, AC, and AB), as previously described. We observed similar percentages of the three major genotypes A (37%), B (33%), and C (27%) in our population. The UL144 genotype distributions were similar among the group of infected adults and the group of infected fetuses and among symptomatic and asymptomatic fetuses (P < 0.05). In our series, all five UL144 genotypes could be vertically transmitted from mothers to fetuses, and all could cause symptomatic congenital infection. We concluded that determination of UL144 polymorphisms in cases of congenital infection is not relevant, since it is unlikely to help predict the outcome of the infection.

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CMV gB genotypes and outcome of vertical transmission: study on dried blood spots of congenitally infected babies

Cited by 81 publications

References 13 publications

Correlation between Infections with Different Genotypes of Human Cytomegalovirus and Epstein-Barr Virus in Subgingival Samples and Periodontal Status of Patients

Correlation between Infections with Different Genotypes of Human Cytomegalovirus and Epstein-Barr Virus in Subgingival Samples and Periodontal Status of Patients

Detection and gB genotyping of CMV in Mexican preterm infants in the context of maternal seropositivity

Human Cytomegalovirus UL144 Gene Polymorphisms in Congenital Infections

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