CMA33/XCT Regulates Small RNA Production through Modulating the Transcription of Dicer-Like Genes in Arabidopsis

Fang, Xiuqi; Shi, Yupeng; Lu, Xiu-Li; Chen, Zulong; Qi, Yijun

doi:10.1016/j.molp.2015.03.002

Cited by 38 publications

(36 citation statements)

References 77 publications

(111 reference statements)

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“…In this particular case the regulation of miRNA levels by SWR1 is the consequence of a direct transcriptional regulation of miRNA genes rather than their processing (Choi et al ., ). Environmental cues are also expected to regulate Dicer‐Like genes given the transcriptional control that CMA33/XCT, a protein previously shown to respond to light and hormone signaling, has over them (Martin‐Tryon and Harmer, ; Ellison et al ., ; Fang et al ., 2015b). The Nuclear Cap‐Binding Complex, which affects many RNA‐related processes, including miRNA biogenesis, also responds to a wide variety of environmental conditions, processes recently reviewed by Daszkowska‐Golec ().…”

Section: Environmental Regulation Of the Mirna Biogenesis Machinerymentioning

confidence: 99%

Keep calm and carry on: miRNA biogenesis under stress

2019

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Summary MicroRNAs (miRNAs) are major post‐transcriptional regulators of gene expression. Their biogenesis relies on the cleavage of longer precursors by a nuclear localized processing machinery. The evolutionary preference of plant miRNAs to silence transcription factors turned these small molecules into key actors during growth and adaptive responses. Furthermore, during their life cycle plants are subject to changes in the environmental conditions surrounding them. In order to face these changes, plants display unique adaptive capacities based on an enormous developmental plasticity, where miRNAs play central roles. Many individual miRNAs have been shown to modulate the plant response to different environmental cues and stresses. In the last few years, increasing evidence has shown that not only individual genes encoding miRNAs but also the miRNA pathway as a whole is subject to regulation in response to external stimulus. In this review, we discuss the current knowledge about the miRNA pathway. We dissect the pathway to analyze the events leading to the generation of these small RNAs and emphasize the regulation of core components of the miRNA biogenesis machinery.

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Section: Environmental Regulation Of the Mirna Biogenesis Machinerymentioning

confidence: 99%

Keep calm and carry on: miRNA biogenesis under stress

2019

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“…Several proteins, CBP80/ABH1, HOS5/SHI1/RCF3, RS40 and RS41, C-TERMINAL DOMAIN PHOSPHATASE-LIKE 1 (CPL1)/FIERY2 (FRY2), RECEPTOR FOR ACTIVATED C KINASE 1 (RACK1), which interact with SE or HYL1, are also required for miRNA generation [17–21]. STABLIZED 1 (STA1) and XAP5 CIRCADIAN TIMEKEEPER (XCT) can modulate miRNA accumulation through regulation of DCL1 transcript level [22, 23]. Several other proteins, such as SICKLE (SIC), Modifier of SNC1, 2 (MOS2), AtGRP7 and THO2 are additional players involved in miRNA accumulation [24–27].…”

Section: Introductionmentioning

confidence: 99%

The SnRK2 kinases modulate miRNA accumulation in Arabidopsis

Yan

Wang

et al. 2017

PLoS Genet

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MicroRNAs (miRNAs) regulate gene expression and play critical roles in growth and development as well as stress responses in eukaryotes. miRNA biogenesis in plants requires a processing complex that consists of the core components DICER-LIKE 1 (DCL1), SERRATE (SE) and HYPONASTIC LEAVES (HYL1). Here we show that inactivation of functionally redundant members of the SnRK2 kinases, which are the core components of abscisic acid (ABA) and osmotic stress signaling pathways, leads to reduction in miRNA accumulation under stress conditions. Further analysis revealed that the steady state level of HYL1 protein in plants under osmotic stress is dependent on the SnRK2 kinases. Additionally, our results suggest that the SnRK2 kinases physically associate with the miRNA processing components SE and HYL1 and can phosphorylate these proteins in vitro. These findings reveal an important role for the SnRK2 kinases in the regulation of miRNA accumulation and establish a mechanism by which ABA and osmotic stress signaling is linked to miRNA biogenesis.

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“…For example, HYL1 activity is controlled by phosphorylation and dephosphorylation (30,31). Furthermore, the expression levels of the DCL1 complex are regulated at transcriptional and posttranscriptional levels to influence miRNA accumulation (32)(33)(34). The structure and length of the stem-loop also impact pri-miRNA processing.…”

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confidence: 99%

STV1, a ribosomal protein, binds primary microRNA transcripts to promote their interaction with the processing complex in Arabidopsis

Liu

Zhang

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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MicroRNAs (miRNAs) are key regulators of gene expression. They are processed from primary miRNA transcripts (pri-miRNAs), most of which are transcribed by DNA-dependent polymerase II (Pol II). miRNA levels are precisely controlled to maintain various biological processes. Here, we report that SHORT VALVE 1 (STV1), a conserved ribosomal protein, acts in miRNA biogenesis in Arabidopsis. A portion of STV1 localizes in the nucleus and binds pri-miRNAs. Using primiR172b as a reporter, we show that STV1 binds the stem-loop flanked by a short 5′ arm within pri-miRNAs. Lack of STV1 reduces the association of pri-miRNAs with their processing complex. These data suggest that STV1 promotes miRNA biogenesis through facilitating the recruitment of pri-miRNAs to their processing complex. Furthermore, we show that STV1 indirectly involves in the occupancy of Pol II at the promoters of miRNA coding genes (MIR) and influences MIR promoter activities. Based on these results, we propose that STV1 refines the accumulation of miRNAs through its combined effects on pri-miRNA processing and transcription. This study uncovers an extraribosomal function of STV1.

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CMA33/XCT Regulates Small RNA Production through Modulating the Transcription of Dicer-Like Genes in Arabidopsis

Cited by 38 publications

References 77 publications

Keep calm and carry on: miRNA biogenesis under stress

Keep calm and carry on: miRNA biogenesis under stress

The SnRK2 kinases modulate miRNA accumulation in Arabidopsis

STV1, a ribosomal protein, binds primary microRNA transcripts to promote their interaction with the processing complex in Arabidopsis

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