CMA Staining Analysis of Chromosomes in Citrus Relatives, Clymenia, Eremocitrus and Microcitrus

Yamamoto, Masashi; Abkenar, Asad Asadi; Matsumoto, Ryoji; Kubo, Tatsuya; Tominaga, Shigeto

doi:10.2503/jjshs1.77.24

Cited by 5 publications

(10 citation statements)

References 21 publications

(29 reference statements)

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“…The PI banding patterns of Citropsis gabunensis, Clymenia polyandra, and Swinglea glutinosa in the present study were identical to those of CMA described in previous reports (Yamamoto et al, 2008a(Yamamoto et al, , 2008b. A resemblance was found between the present PI results and the previous CMA banding patterns of Citrus reshni, Fortunella japonica, and Poncirus trifoliata (Befu et al, 2000;Brasileiro-Vidal et al, 2007;Cornelio et al, 2003;Kunitake et al, 2005;Yamamoto and Tomoinaga, 2003).…”

Section: Discussionsupporting

confidence: 78%

“…It is considered that Clymenia is the most primitive of all the genera of true citrus fruit trees due to its morphological traits (Swingle and Reece, 1967). Its chromosome configuration seemed to be the primitive type of true citrus fruit trees (Yamamoto et al, 2008a). Swinglea glutinosa, which lacks a heterochromatin rich chromosome, is considered to have the most primitive chromosome configuration in Aurantioideae (Yamamoto et al, 2008b).…”

Section: Discussionmentioning

confidence: 99%

“…Citrus chromosomes have been found to exhibit a high degree of diversity and heterozygosity, an understanding of which has shed light on the phylogenic relationships of Citrus and its related genera (Befu et al, 2001;Cornelio et al, 2003;Guerra et al, 2000;Miranda et al, 1997b;Yamamoto et al, 2007Yamamoto et al, , 2008aYamamoto et al, , 2008bYamamoto and Tominaga, 2003).…”

Section: Introductionmentioning

confidence: 99%

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Physical Mapping of the 5S Ribosomal RNA Gene in Citreae of Aurantioideae Species using Fluorescence in situ Hybridization

Yamamoto

Abkenar

Matsumoto

et al. 2009

J. Japan. Soc. Hort. Sci.

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The location of the 5S ribosomal RNA gene (rDNA) in species from six genera of the Citreae of Aurantioideae was determined using fluorescence in situ hybridization (FISH). A 5S rDNA probe was labeled with biotin-16-dUTP. The probe was detected using a fluorescein isothiocyanate (FITC)-avidin conjugate with chromosomes counterstained with propidium iodide (PI). When the chromosomes were observed under a G filter, PI-stained chromosomes were classified into the following five types based on the number and position of PI-positive (+) bands; B: one telomeric and one proximal band, C: two telomeric bands, D: one telomeric band, E: without bands and Dst: type D with a satellite chromosome. Two 5S rDNA sites were located in type D chromosomes in Citropsis gabunensis, whereas in Citrus reshni, Fortunella japonica, Clymenia polyandra and Swinglea glutinosa they were found in type E chromosomes. In Poncirus trifoliata, three rDNA sites were located together at the proximal PI (+) band of a type B chromosome, one site was located in the proximal region of a type D chromosome, and two sites were located together at the PI (+) band of a type D chromosome.

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Section: Discussionsupporting

confidence: 78%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Physical Mapping of the 5S Ribosomal RNA Gene in Citreae of Aurantioideae Species using Fluorescence in situ Hybridization

Yamamoto

Abkenar

Matsumoto

et al. 2009

J. Japan. Soc. Hort. Sci.

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“…Looking at the vast diversity of CMA banding patterns in Citrus accessions and related genera it becomes clear that some patterns occur two or more times among species of Citrus and other 20 Aurantioideae genera investigated Yamamoto et al 2008). For example, among the few species investigated here, T. trifolia and C. sunki (15D + 3F and 14D + 4F, respectively), and M. paniculata and C. deliciosa (2C + 14D + 2F and 2C + 12D + 4F, respectively), displayed similar CMA banding patterns.…”

Section: Discussionmentioning

confidence: 99%

The evolution of CMA bands in Citrus and related genera

et al. 2010

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Most species of Citrus and related genera display a similar karyotype with 2n = 18 and a variable number of terminal heterochromatic blocks positively stained with chromomycin A(3) (CMA(+) bands). Some of these blocks are 45S rDNA sites, whereas others may correspond to the main GC-rich satellite DNA found in several Citrus species. In the present work, the distribution of the 45S rDNA and the main satellite DNA isolated from C. sinensis (CsSat) were investigated by in situ hybridization in seven species of Citrus, two species of closely related genera (Fortunella obovata and Poncirus trifoliata) and four species of the subfamily Aurantioideae, which were less related to Citrus (Atalantia monophylla, Murraya paniculata, Severinia buxifolia, and Triphasia trifolia). In Citrus, Fortunella, and Poncirus, most CMA(+) bands colocalized only with CsSat sites, whereas others colocalized only with rDNA sites. However, some of these species displayed a few CMA(+) bands that colocalized with sites of both probes and other CMA(+) bands that did not colocalized with any of the probes. On the other hand, in the four species less related to Citrus, no CsSat signal was found on chromosomes. On Southern blot, the CsSat probe hybridized with genomic DNA from Citrus, Fortunella, and Poncirus at high stringency only, while under the less stringent conditions, it also hybridized with distantly related species. Therefore, CsSat sequences are the principal component of the heterochromatic blocks of Citrus, Poncirus, and Fortunella, whereas CsSat-like sequences seem to be widespread in the subfamily Aurantioideae. These data further suggest that the variable number of terminal CMA(+) bands observed on chromosomes of Citrus and related genera are probably the consequence of amplification or reduction in the number of CsSat-like sequences distributed on chromosome termini, paralleled by mutation and homogenization events, as proposed by the library hypothesis.

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“…CMA banding pattern has also been described for several other genera of Aurantioideae [see e.g. Guerra et al, 2000;Yamamoto et al, 2008]. A better chromosome differentiation of Citrus species was obtained when CMA/DAPI stained metaphases were sequentially in situ hybridized with 5S and 45S rDNA probes.…”

mentioning

confidence: 86%

Linked 5S and 45S rDNA Sites Are Highly Conserved through the Subfamily Aurantioideae (Rutaceae)

Silva

Filho

Guerra

2013

Cytogenet Genome Res

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Sites of 5S and 45S rDNA are more commonly located on different chromosomes of most angiosperms. Previous investigations have shown that in the subfamily Aurantioideae these sites may appear closely linked (adjacent sites), as in Poncirustrifoliata, or completely isolated, as in some species of Citrus. In the present work, the distribution of rDNA sites was investigated in representatives of 9 genera of Aurantioideae by FISH and CMA banding, aiming to understand the evolution of adjacent sites in the subfamily. A total of 57 rDNA sites were observed, 40 of them being adjacent to each other. All adjacent sites displayed the 45S rDNA array more terminally located. Assuming that the linked 5S-45S rDNA arrangement was the ancestral condition in Aurantioideae, the isolated rDNA sites observed in Clausena excavata,Bergera koenigii, and Fortunella obovata, as well as the complete linkage loss in Citrus maxima and C. medica indicates that unlinked sites arose independently several times in the evolution of the group. The linkage loss may be due to independent dispersion of one or both rDNA sequence families followed by deletion of the corresponding array in the adjacent site. The possible mechanisms behind these events and their occurrence in other groups are discussed.

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CMA Staining Analysis of Chromosomes in Citrus Relatives, Clymenia, Eremocitrus and Microcitrus

Cited by 5 publications

References 21 publications

Physical Mapping of the 5S Ribosomal RNA Gene in Citreae of Aurantioideae Species using Fluorescence in situ Hybridization

Physical Mapping of the 5S Ribosomal RNA Gene in Citreae of Aurantioideae Species using Fluorescence in situ Hybridization

The evolution of CMA bands in Citrus and related genera

Linked 5S and 45S rDNA Sites Are Highly Conserved through the Subfamily Aurantioideae (Rutaceae)

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