Clusters of polymersomes and Janus nanoparticles hierarchically self-organized and controlled by DNA hybridization

Mihali, Voichiţa; Skowicki, Michal; Messmer, Daniel; Palivan, Cornelia G.

doi:10.1016/j.nantod.2022.101741

Cited by 6 publications

(4 citation statements)

References 94 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Pol-Atto633 exhibited a slightly negative zeta potential (−4.0 ± 0.5 mV), in good agreement with the values obtained for polymersomes assembled using mixtures of PDMS- b -PMOXA copolymers (Table S2). We calculated an encapsulation efficiency of Atto633 of 47% ± 3%, assessed by UV spectroscopy, which is in agreement with values obtained for the encapsulation of small molecular weight molecules inside polymersomes during the self-assembly process. , …”

Section: Resultssupporting

confidence: 87%

FAP Targeting of Photosensitizer-Loaded Polymersomes for Increased Light-Activated Cell Killing

Skowicki,

Hürlimann,

Tarvirdipour

et al. 2024

Biomacromolecules

Self Cite

View full text Add to dashboard Cite

As current chemo-and photodynamic cancer therapies are associated with severe side effects due to a lack of specificity and to systemic toxicity, innovative solutions in terms of targeting and controlled functionality are in high demand. Here, we present the development of a polymersome nanocarrier equipped with targeting molecules and loaded with photosensitizers for efficient uptake and light-activated cell killing. Polymersomes were self-assembled in the presence of photosensitizers from a mixture of nonfunctionalized and functionalized PDMS-b-PMOXA diblock copolymers, the latter designed for coupling with targeting ligands. By encapsulation inside the polymersomes, the photosensitizer Rose Bengal was protected, and its uptake into cells was mediated by the nanocarrier. Inhibitor of fibroblast activation protein α (FAPi), a ligand for FAP, was attached to the polymersomes' surface and improved their uptake in MCF-7 breast cancer cells expressing relatively high levels of FAP on their surface. Once internalized by MCF-7, irradiation of Rose Bengal-loaded FAPipolymersomes generated reactive oxygen species at levels high enough to induce cell death. By combining photosensitizer encapsulation and specific targeting, polymersomes represent ideal candidates as therapeutic nanocarriers in cancer treatment.

show abstract

Section: Resultssupporting

confidence: 87%

FAP Targeting of Photosensitizer-Loaded Polymersomes for Increased Light-Activated Cell Killing

Skowicki,

Hürlimann,

Tarvirdipour

et al. 2024

Biomacromolecules

Self Cite

View full text Add to dashboard Cite

show abstract

“…10 Of particular interest are solid-supported membranes (SSMs) that result from the self-assembly of amphiphilic copolymers on a solid support 11 because of their increased mechanical stability compared to lipidic mem-branes, 12,13 their chemical versatility, their tunability, as well as their reproducibility. 14,15 If appropriately selected, amphiphilic block copolymers form flexible membranes that enable the insertion of membrane proteins, despite being thicker than lipid-based membranes. 16 In the pursuit of fully synthetic mimics of cell function, there is a quest for new building blocks that allow for selective crossmembrane transport.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Similar to their naturally occurring counterparts, the resulting biomimetic membranes are endowed with functionalities such as selective permeability, active transport, catalytic activity, or molecular recognition . Of particular interest are solid-supported membranes (SSMs) that result from the self-assembly of amphiphilic copolymers on a solid support because of their increased mechanical stability compared to lipidic membranes, , their chemical versatility, their tunability, as well as their reproducibility. , If appropriately selected, amphiphilic block copolymers form flexible membranes that enable the insertion of membrane proteins, despite being thicker than lipid-based membranes …”

Section: Introductionmentioning

confidence: 99%

Planar Polymer Membranes Accommodate Functional Self-Assembly of Inserted Resorcinarene Nanocapsules

Muthwill,

Bina,

Paracini

et al. 2024

ACS Appl. Mater. Interfaces

Self Cite

View full text Add to dashboard Cite

Solid-supported polymer membranes (SSPMs) offer great potential in material and life sciences due to their increased mechanical stability and robustness compared to solidsupported lipid membranes. However, there is still a need for expanding the functionality of SSPMs by combining them with synthetic molecular assemblies. In this study, SSPMs served as a flexible matrix for the insertion of resorcinarene monomers and their self-assembly into functional hexameric resorcinarene capsules. Resorcinarene capsules provide a large cavity with affinity specifically for cationic and polyhydroxylated molecules. While the capsules are stable in apolar organic solvents, they disassemble when placed in polar solvents, which limits their application. Here, a solvent-assisted approach was used for copolymer membrane deposition on solid support and simultaneous insertion of the resorcinarene monomers. By investigation of the molecular factors and conditions supporting the codeposition of the copolymer and resorcinarene monomers, a stable hybrid membrane was formed. The hydrophobic domain of the membrane played a crucial role by providing a sufficiently thick and apolar layer, allowing for the self-assembly of the capsules. The capsules were functional inside the membranes by encapsulating cationic guests from the aqueous environment. The amount of resorcinarene capsules in the hybrid membranes was quantified by a combination of quartz-crystal microbalance with dissipation and liquid chromatography−mass spectrometry, while the membrane topography and layer composition were analyzed by atomic force microscopy and neutron reflectometry. Functional resorcinarene capsules inside SSPMs can serve as dynamic sensors and potentially as cross-membrane transporters, thus holding great promise for the development of smart surfaces.

show abstract

“…[ 4 ] Driven by various self‐assembly forces, multiple polymers with exquisite structures have emerged, which have great potential as functional biomaterials in disease therapy, [ 5 ] drug delivery, [ 6 ] chemical sensing, [ 7 ] and catalysis. [ 8 , 9 ] However, previously reported polymers often require special molecular monomer designs (e.g., peptides, [ 10 ] DNA sequences, [ 11 ] and protein region [ 12 ] ) or highly specific mechanisms to control intermolecular interactions (e.g., metal‐organic coordination, [ 13 ] DNA hybridization, [ 14 ] and antibody‐antigen specificity [ 15 ] ) to trigger assembly. [ 16 ] And these assembly processes are also subject to external physical factors, resulting in limited and high‐demand synthetic conditions ( Figure 1 A ).…”

Section: Introductionmentioning

confidence: 99%

Hierarchical Self‐Assembly Molecular Building Blocks as Intelligent Nanoplatforms for Ovarian Cancer Theranostics

Li,

Chen,

et al. 2024

Advanced Science

View full text Add to dashboard Cite

Hierarchical self‐assembly from simple building blocks to complex polymers is a feasible approach to constructing multi‐functional smart materials. However, the polymerization process of polymers often involves challenges such as the design of building blocks and the drive of external energy. Here, a hierarchical self‐assembly with self‐driven and energy conversion capabilities based on p‐aminophenol and diethylenetriamine building blocks is reported. Through β‐galactosidase (β‐Gal) specific activation to the self‐assembly, the intelligent assemblies (oligomer and superpolymer) with excellent photothermal and fluorescent properties are dynamically formed in situ, and thus the sensitive multi‐mode detection of β‐Gal activity is realized. Based on the overexpression of β‐Gal in ovarian cancer cells, the self‐assembly superpolymer is specifically generated in SKOV‐3 cells to achieve fluorescence imaging. The photothermal therapeutic ability of the self‐assembly oligomer (synthesized in vitro) is evaluated by a subcutaneous ovarian cancer model, showing satisfactory anti‐tumor effects. This work expands the construction of intelligent assemblies through the self‐driven cascade assembly of small molecules and provides new methods for the diagnosis and treatment of ovarian cancer.

show abstract

Clusters of polymersomes and Janus nanoparticles hierarchically self-organized and controlled by DNA hybridization

Cited by 6 publications

References 94 publications

FAP Targeting of Photosensitizer-Loaded Polymersomes for Increased Light-Activated Cell Killing

FAP Targeting of Photosensitizer-Loaded Polymersomes for Increased Light-Activated Cell Killing

Planar Polymer Membranes Accommodate Functional Self-Assembly of Inserted Resorcinarene Nanocapsules

Hierarchical Self‐Assembly Molecular Building Blocks as Intelligent Nanoplatforms for Ovarian Cancer Theranostics

Contact Info

Product

Resources

About