Clustering of catalytic nanocompartments for enhancing an extracellular non-native cascade reaction

Maffeis, Viviana; Belluati, Andrea; Craciun, Ioana; Wu, Dalin; Novak, Samantha; Schoenenberger, Cora‐Ann; Palivan, Cornelia G.

doi:10.1039/d1sc04267j

Cited by 33 publications

(50 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[41][42][43] A porous membrane was pivotal to our CNC design in order to provide a pathway for hydrophilic substrates and products to and from the nanocompartment cavity containing GUS. 41,43,44 In the present study, we report the physicochemical characterization of GUS CNCs and their enzymatic efficiency in phosphate buffered solution (PBS) and cell culture medium. To explore the potential of CNCs for therapeutic applications, we examined cellular toxicity, uptake into cells and intracellular activity of GUS CNCs.…”

Section: Introductionmentioning

confidence: 99%

Inverting glucuronidation of hymecromone in situ by catalytic nanocompartments

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Introductionmentioning

confidence: 99%

Inverting glucuronidation of hymecromone in situ by catalytic nanocompartments

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

“…One approach to achieve external attachment of molecules is to introduce the desired chemical or reactive groups to the end of the hydrophilic copolymer block during the polymerization process [ 103 ]. The functional end groups often include hydroxyl [ 91 , 103 ], amine [ 104 ], and N-hydroxysuccimidyl esters [ 105 ]. Then, by using a mixture of the end-functionalized block copolymer with a nonfunctionalized copolymer at the desired molar ratio and the methods described above, compartments bearing functional groups are formed.…”

Section: Generation Of Synthetic Compartmentsmentioning

confidence: 99%

“…Compartments have been used to generate more complex assemblies, either by encapsulating nanoassemblies into GUVs (compartments-in-compartments architecture) [ 67 , 71 , 92 ] or by the association of compartments and formation of networks and clusters [ 91 , 103 , 120 ]. Compartment-in-compartment architectures include various combinations of functional polymer or lipid membranes and multilayer capsules enclosed in a GUV membrane within which they can interact.…”

Section: Generation Of Synthetic Compartmentsmentioning

confidence: 99%

“…While such systems allow more controlled cascade reactions, the encapsulation efficiency of nanocompartments into GUVs is still low, which generates issues of sensitivity [ 126 ]. On the contrary, clusters, which are formed by zipping together compartments into a structure similar to that of cell colonies, have an architecture that supports an increase in the encapsulation efficiency while allowing for the location of different molecules in segregated spaces at the nano scale [ 91 , 103 , 120 ]. Bioconjugation based on nucleic acid hybridization has been applied to both liposomes [ 127 ] and polymersomes [ 125 ].…”

Section: Generation Of Synthetic Compartmentsmentioning

confidence: 99%

“…Clusters of CNCs encapsulating glucose oxidase (Gox; Gox-CNCs) and lactoperoxidase (LPO; LPO-CNCs) can be used for a cascade reaction that functions as protection from lung infections, treatment of hyperglycemia, and reactive oxygen species (ROS) therapy [ 91 ]. The clusters were tested on lung carcinoma epithelial cells, where they successfully consumed glucose.…”

Section: Applications Of Compartments In the Biomedical Fieldmentioning

confidence: 99%

See 2 more Smart Citations

Current Perspectives on Synthetic Compartments for Biomedical Applications

Heuberger

Korpidou

Eggenberger

et al. 2022

IJMS

Self Cite

View full text Add to dashboard Cite

Nano- and micrometer-sized compartments composed of synthetic polymers are designed to mimic spatial and temporal divisions found in nature. Self-assembly of polymers into compartments such as polymersomes, giant unilamellar vesicles (GUVs), layer-by-layer (LbL) capsules, capsosomes, or polyion complex vesicles (PICsomes) allows for the separation of defined environments from the exterior. These compartments can be further engineered through the incorporation of (bio)molecules within the lumen or into the membrane, while the membrane can be decorated with functional moieties to produce catalytic compartments with defined structures and functions. Nanometer-sized compartments are used for imaging, theranostic, and therapeutic applications as a more mechanically stable alternative to liposomes, and through the encapsulation of catalytic molecules, i.e., enzymes, catalytic compartments can localize and act in vivo. On the micrometer scale, such biohybrid systems are used to encapsulate model proteins and form multicompartmentalized structures through the combination of multiple compartments, reaching closer to the creation of artificial organelles and cells. Significant progress in therapeutic applications and modeling strategies has been achieved through both the creation of polymers with tailored properties and functionalizations and novel techniques for their assembly.

show abstract

Polymeric Giant Unilamellar Vesicles with Integrated DNA‐Origami Nanopores: An Efficient Platform for Tuning Bioreaction Dynamics Through Controlled Molecular Diffusion

Cochereau

Maffeis

Santos

et al. 2023

Adv Funct Materials

Self Cite

View full text Add to dashboard Cite

Giant unilamellar vesicles (GUVs) are microcompartments serving to confine reactions, allow signaling pathways, or design synthetic cells. Polymer GUVs are composed of copolymer membranes mimicking cell membranes, and present advantages over lipid‐based GUVs, such as higher mechanical stability and chemical versatility. Such microcompartments are essential for understanding reactions/signaling occurring in cells, which are difficult to study by in vivo approaches due to the cell's complexity. However, the lack of control over their production, stability, and membrane diffusion properties is still limiting their use for bio‐related applications. Here, polymer GUVs produced by microfluidics and permeabilized with DNA‐origami nanopores (DoNs) that present a high level of control over these essential properties are introduced. After systematic optimization of conditions, DoN‐GUVs reveal a narrow size distribution, allow for high encapsulation efficiencies, and are stable for weeks, protecting encapsulated biomolecules. The kinetics of diffusion of molecules through the GUV's membrane is tuned by insertion of DoNs with a controlled 3D‐ structure. DNA polymerase I, encapsulated as model for bioreactions, successfully produced DNA duplex strands with spatiotemporal control. DoN‐GUVs loaded with active molecules open new avenues in bioreactions, from the detection of biomolecules, over the tuning of molecular transport rates, to the investigation of cellular processes/signaling.

show abstract

Clustering of catalytic nanocompartments for enhancing an extracellular non-native cascade reaction

Abstract: Compartmentalization is fundamental in nature, where the spatial segregation of biochemical reactions within and between cells ensures optimal conditions for the regulation of cascade reactions.

Cited by 33 publications

References 60 publications

Inverting glucuronidation of hymecromone in situ by catalytic nanocompartments

Inverting glucuronidation of hymecromone in situ by catalytic nanocompartments

Current Perspectives on Synthetic Compartments for Biomedical Applications

Polymeric Giant Unilamellar Vesicles with Integrated DNA‐Origami Nanopores: An Efficient Platform for Tuning Bioreaction Dynamics Through Controlled Molecular Diffusion

Contact Info

Product

Resources

About