Clustered Regularly Interspaced Short Palindromic Repeats-Associated Protein System for Resistance Against Plant Viruses: Applications and Perspectives

Silva, Fredy D.A.; Fontes, Elizabeth P. B.

doi:10.3389/fpls.2022.904829

Cited by 4 publications

(5 citation statements)

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“…However, nowadays, a new genome editing platform has been successfully used, which has surpassed the efficiency of ZFNs and TALEN in plants. According to scientists, CRISPR/Cas is currently the most powerful biological tool for targeted genome modification ( Silva and Fontes, 2022 ; Tiwari et al., 2022 ). Many researchers consider the CRISPR/Cas-mediated immunity mechanism based on regularly spaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) proteins crucial in the fight against pathogens.…”

Section: Biotechnological Approaches To Countering Phytovirusesmentioning

confidence: 99%

“…The creation of next-generation systems characterizes the current stage of development of CRISPR methods such as CRISPR-MAD7 and CAS12A nucleases, increasing accuracy, range of possibilities, and applications. The efficiency of the CRISPR-MAD7 system has been proven on mutant rice and wheat plants at 65.6% ( Silva and Fontes, 2022 ). Using MAD7 expands the CRISPR genome-editing toolkit because of its highly efficient target for gene disruption and insertion.…”

Section: Biotechnological Approaches To Countering Phytovirusesmentioning

confidence: 99%

“…Using MAD7 expands the CRISPR genome-editing toolkit because of its highly efficient target for gene disruption and insertion. According to ( Silva and Fontes, 2022 ), using the new CRISPR/CAS systems (CAS3, CAS12, CAS13, and CAS14) as multiplexed SGRNAs by targeting them to different sites is a new and more effective strategy for increasing resistance to a wide range of viral infections and disease control in the field.…”

Section: Biotechnological Approaches To Countering Phytovirusesmentioning

confidence: 99%

“…Sweet potato (Ipomoea batatas) 80%-98% (Mwanga et al, 2001;Clark et al, 2012;Alam et al, 2013) 30%-50% (Silva and Fontes, 2022) Tomatoes (Solanum lycopersicum) 42.1%-95.5% (Hossain et al, 2011;Sevik and Arli-Sokmen, 2012;Farooq et al, 2021) Okra (Abelmoschus esculentus L. Moench) 49%-84% (Mishra et al, 2017) 94% (Dhankhar, 2016) Potato (Solanum tuberosum) 10%-80% (Mumford et al, 2016;Kreuze et al, 2020) 10% (Moses et al, 2017) 10%-90% (Salazar, 1996) 15%-75% (Gong et al, 2019) Pepper (Capsicum annuum) 70%-80% (Tolkach et al, 2019) Watermelon (Citrullus lanatus) 10%-75% (Xu et al, 2004;Tolkach et al, 2019) Melon (Cucumis melo L.) 80% (Saéz et al, 2022) 30%-60 (Alonso-Prados et al, 1997) Wheat (Triticum) 80% (Perry et al, 2000) 41%-63% (Cisar et al, 1982) the transpiration intensity and the water inflow due to changes in the vascular system (Anikina and Seitzhanova, 2015). The transpiration flow slows down, and the intensity of transpiration decreases, resulting from changes in the conductive system.…”

Section: Crop Decrease In Productivity Sourcementioning

confidence: 99%

“…Method Source 1 Genome editing to combat virus infection (Shan et al, 2013;Baltes et al, 2014;Price et al, 2015;Abudayyeh et al, 2017;Wolter and Puchta, 2018;Zhang et al, 2018;Zhan et al, 2019;Ahmad et al, 2020;Nussenzweig and Marraffini, 2020;Zhao et al, 2020;Nidhi et al, 2021;Hofvander et al, 2022;Kavuri et al, 2022;Robertson et al, 2022;Sharma et al, 2022;Silva and Fontes, 2022;Tiwari et al, 2022) 2 Apical meristem method (Zhu-Jun et al, 2011;Hull, 2014;Anikina and Seitzhanova, 2015;Jin et al, 2016;Antonova et al, 2017;Galeev et al, 2018;Zhang et al, 2019) 3 Thermotherapy (Wu et al, 2015;Oves and Gaytova, 2016;Wang et al, 2022) 4 Combination of thermotherapy methods and the method of apical meristems (Wang et al, 2006;AlMaarri et al, 2012;Moses et al, 2017) 5 Cryotherapy (Wang et al, 2006;Yi et al, 2014;Bi et al, 2018;Zhang et al, 2019) 6 Chemotherapy (AlMaarri et al, 2012;Ryabtseva et al, 2015;Antonova et al, 2017;Yalovik et al, 2019;Bettoni et al, 2022) 7 Combination of thermotherapy and chemotherapy methods (Fletcher et al, 1998;…”

Section: S Nomentioning

confidence: 99%

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Plant protection from virus: a review of different approaches

Anikina,

Kamarova,

Issayeva

et al. 2023

Front. Plant Sci.

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This review analyzes methods for controlling plant viral infection. The high harmfulness of viral diseases and the peculiarities of viral pathogenesis impose special requirements regarding developing methods to prevent phytoviruses. The control of viral infection is complicated by the rapid evolution, variability of viruses, and the peculiarities of their pathogenesis. Viral infection in plants is a complex interdependent process. The creation of transgenic varieties has caused much hope in the fight against viral pathogens. The disadvantages of genetically engineered approaches include the fact that the resistance gained is often highly specific and short-lived, and there are bans in many countries on the use of transgenic varieties. Modern prevention methods, diagnosis, and recovery of planting material are at the forefront of the fight against viral infection. The main techniques used for the healing of virus-infected plants include the apical meristem method, which is combined with thermotherapy and chemotherapy. These methods represent a single biotechnological complex method of plant recovery from viruses in vitro culture. It widely uses this method for obtaining non-virus planting material for various crops. The disadvantages of the tissue culture-based method of health improvement include the possibility of self-clonal variations resulting from the long-term cultivation of plants under in vitro conditions. The possibilities of increasing plant resistance by stimulating their immune system have expanded, which results from the in-depth study of the molecular and genetic bases of plant resistance toward viruses and the investigation of the mechanisms of induction of protective reactions in the plant organism. The existing methods of phytovirus control are ambiguous and require additional research. Further study of the genetic, biochemical, and physiological features of viral pathogenesis and the development of a strategy to increase plant resistance to viruses will allow a new level of phytovirus infection control to be reached.

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Section: Biotechnological Approaches To Countering Phytovirusesmentioning

confidence: 99%

Section: Biotechnological Approaches To Countering Phytovirusesmentioning

confidence: 99%

Section: Biotechnological Approaches To Countering Phytovirusesmentioning

confidence: 99%