Cluster of bloodstream infections caused by KPC-2 carbapenemase-producing Klebsiella pneumoniae in Manhattan

Nadkarni, Abhijeet S.; Schliep, Tjark; Khan, Lamia; Zeana, Cosmina B.

doi:10.1016/j.ajic.2007.10.013

Cited by 48 publications

(31 citation statements)

References 31 publications

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“…Indeed, high mortality rates, up to 66%, were associated with bacteremia due to bla KPC -positive bacteria (5,20). The high mortality rate may be attributed to multiple resistance mechanisms that are associated with bla KPC -positive bacteria (21).…”

mentioning

confidence: 99%

“…The high mortality rate may be attributed to multiple resistance mechanisms that are associated with bla KPC -positive bacteria (21). bla KPC -positive bacteria have been implicated in many cases of hospital-acquired infections, which mandates the rapid detection of these pathogens by the clinical laboratory in order to control their spread (16,20,30). The bla KPC enzymes are encoded in large plasmids that vary in size and structure.…”

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confidence: 99%

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Rapid Detection of bla _KPC Carbapenemase Genes by Internally Controlled Real-Time PCR Assay Using Bactec Blood Culture Bottles

et al. 2011

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Rapid detection of drug-resistant bacteria in clinical samples plays an instrumental role in patients'infection management and in implementing effective infection control policies. In the study described in this report, we validated a multiplex TaqMan real-time quantitative PCR (qPCR) assay for the detection of bla KPC genes and the human RNase P gene in Bactec blood culture bottles. The MagNA Pure LC (version 2.0) instrument was utilized to extract nucleic acids from the inoculated broth, while bovine serum albumin (BSA) was utilized as the PCR inhibitor reliever. The multiplex assay, which was specific for the detection of bla KPC genes, had a limit of detection of 19 CFU per reaction mixture with human blood-spiked Bactec bottles. Of the 323 Bactec blood culture sets evaluated, the same 55 (17%) blood cultures positive for carbapenem-resistant bacteria by culture were also positive by the validated qPCR assay. Thus, the sensitivity, specificity, positive predictive value, and negative predictive value of the qPCR assay compared to the results of culture were all 100%. bla KPC genes were also detected from the same Bactec bottle broth after manual extraction with a QIAamp DNA minikit; however, there was an average 3-threshold-cycle delay in the qPCR readings. With the limited therapeutic options available, the accurate and rapid detection of bla KPC -possessing bacteria by the described bla KPC /RNase P assay will be a crucial first step in ensuring optimal clinical outcomes and infection control.

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Rapid Detection of bla _KPC Carbapenemase Genes by Internally Controlled Real-Time PCR Assay Using Bactec Blood Culture Bottles

et al. 2011

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“…In a study done by Mumtaz S et al, 11 , antibiotic sensitivity correlates with our study where most of the isolates are sensitive to Imipenem (95.7%) followed by Amikacin (76%).…”

Section: Resultsmentioning

confidence: 53%

“…The studies which are listed in Table 5 conducted in wide geographical areas of India by various authors, from the past 12 years reported a widely varied prevalence ranging from 10.10% to 87%. [7][8][9][10][11][12][13][14][15][16][17][18][19] Major risk factors for colonization or infection with ESBL producing organisms are long term antibiotic exposure, prolonged ICU stay, nursing home residency, severe illness, residence in an institution with high rates of Ceftazidime and other 3-GC use and instrumentation or catherization. 4 Though ESBLs are quite prevalent in Indian hospitals and community, they are not routinely tested by diagnostic Microbiology laboratories.…”

Section: Resultsmentioning

confidence: 99%

Prevalence of ESBL Producing Escherichia coli and Klebsiella Species among Clinical Isolates and their in vitro Antimicrobial Susceptibility Pattern in a Tertiary Care Hospital

Gupta¹,

Maheshwari²,

Shah³

2017

Int.J.Curr.Microbiol.App.Sci

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“…seems to be an emerging public health problem in various parts of the world [30,38], although, to date, widespread hospital outbreaks have been reported mainly in the United States [11,26,46,51,[63][64][65][66][67] and Israel [8,43].…”

Section: Discussionmentioning

confidence: 99%

The KPC type beta-lactamases: new enzymes that confer resistance to carbapenems in Gram-negative bacilli.

Sacha

Ostas²,

Jaworowska³

et al. 2010

Folia Histochem Cytobiol.

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Antimicrobial resistance due to the continuous selective pressure from widespread use of antimicrobials in humans, animals and agriculture has been a growing problem for last decades. KPC β-lactamases hydrolyzed β-lactams of all classes. Especially, carbapenem antibiotics are hydrolyzed more efficiency than other β-lactam antibiotics. The KPC enzymes are found most often in Enterobacteriaceae. Recently, these enzymes have been found in isolates of Pseudomonas aeruginosa and Acinetobacter spp. The observations of bla KPC genes isolated from different species in other countries indicate that these genes from common but unknown ancestor may have been mobilized in these areas or that bla KPC -carrying bacteria may have been passively by many vectors. The emergence of carbapenem resistance in Gram-negative bacteria is worrisome because the carbapenem resistance often may be associated with resistance to many β-lactam and non-β-lactam antibiotics. Treatment of infections caused by KPC-producing bacteria is extremely difficult because of their multidrug resistance, which results in high mortality rates. Therapeutic options to treat infections caused by multiresistant Gram-negative bacteria producing KPC-carbapenemases could be used polymyxin B or tigecycline.

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Cluster of bloodstream infections caused by KPC-2 carbapenemase-producing Klebsiella pneumoniae in Manhattan

Cited by 48 publications

References 31 publications

Rapid Detection of bla _KPC Carbapenemase Genes by Internally Controlled Real-Time PCR Assay Using Bactec Blood Culture Bottles

Rapid Detection of bla _KPC Carbapenemase Genes by Internally Controlled Real-Time PCR Assay Using Bactec Blood Culture Bottles

Prevalence of ESBL Producing Escherichia coli and Klebsiella Species among Clinical Isolates and their in vitro Antimicrobial Susceptibility Pattern in a Tertiary Care Hospital

The KPC type beta-lactamases: new enzymes that confer resistance to carbapenems in Gram-negative bacilli.

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Cluster of bloodstream infections caused by KPC-2 carbapenemase-producing Klebsiella pneumoniae in Manhattan

Cited by 48 publications

References 31 publications

Rapid Detection of bla KPC Carbapenemase Genes by Internally Controlled Real-Time PCR Assay Using Bactec Blood Culture Bottles

Rapid Detection of bla KPC Carbapenemase Genes by Internally Controlled Real-Time PCR Assay Using Bactec Blood Culture Bottles

Prevalence of ESBL Producing Escherichia coli and Klebsiella Species among Clinical Isolates and their in vitro Antimicrobial Susceptibility Pattern in a Tertiary Care Hospital

The KPC type beta-lactamases: new enzymes that confer resistance to carbapenems in Gram-negative bacilli.

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Rapid Detection of bla _KPC Carbapenemase Genes by Internally Controlled Real-Time PCR Assay Using Bactec Blood Culture Bottles

Rapid Detection of bla _KPC Carbapenemase Genes by Internally Controlled Real-Time PCR Assay Using Bactec Blood Culture Bottles