Cluster-Level Relationships of Genes Involved in Carbon Metabolism in Synechocystis sp. PCC 6803: Development of a Novel Succinate-Producing Strain

Abstract

We quantified the transcript levels of 44 genes related to sugar catabolism in strains with altered primary carbon metabolism and discovered a consistent expression pattern among succinate-producing mutants. To identify factors that determine the expression pattern, we calculated Pearson's correlation coefficients, using the transcript data. Correlation analysis revealed positive and negative correlations among genes encoding sugar catabolic enzymes. On the basis of this analysis, we found that the mutant overexpressing both rre37 (encoding an OmpR-type response regulator) and sigE (encoding an RNA polymerase sigma factor) produced increased levels of succinate under dark, anaerobic conditions, with a maximum productivity of 420 mg l-1.

Simultaneous increases in the levels of compatible solutes by cost‐effective cultivation of Synechocystis sp. PCC 6803

“…Metabolomic techniques for assessment of Synechocystis 6803 cells have also been applied to cyanobacteria for the production of ethanol (Yoshikawa et al, 2017), succinate and lactate (Hasunuma et al, 2018;Osanai et al, 2015b), PHB (Arisaka et al, 2019;Monshupanee et al, 2019), isoprene (Pade et al, 2016), fatty acids (Kodama et al, 2018), and glycogen (Hasunuma et al, 2014). Integrated "omics" analysis and systems biology approaches are used to understand the effects of diurnal cycles (Angermayr et al, 2016;Saha et al, 2016), stress conditions (Zhu et al, 2013), light/dark transitions (Wan et al, 2017), and genetic manipulations (Takeya et al, 2018). These metabolite analyses revealed the metabolic status of Synechocystis 6803 cells and helped increase the production of metabolites of interest.…”

A desiccated dual-species subaerial biofilm reprograms its metabolism and affects water dynamics in limestone

“…Acetate and lactate production could also be derived from a shift toward the oxidative pentose pathway of Synechocystis sp. PCC 6803 (Takeya et al, 2018). Accumulation of 2-hydroxy-3-methylvalerate could be due to the fermentation shift.…”

Glycosyltransferase Slr1064 regulates carbon metabolism by modulating the levels of UDP‐GlcNAc in Synechocystis sp. PCC 6803

“…Despite some GT genes being differentially expressed across various trophic conditions (Fang et al ., 2017), the involvement of GTs in regulating carbon metabolism in Synechocystis has not been reported. Carbon metabolism in Synechocystis is intricately linked to photosynthesis and plays a crucial role in the bioproduction of high‐value products as well as the global carbon cycle (Sarkar & Shimizu, 2015; Takeya et al ., 2018). The intricate network making up carbon metabolism mainly comprises the Calvin cycle, gluconeogenesis, glycolysis, and the oxidative pentose phosphate pathway (Yang et al ., 2002; Young et al ., 2011; Yoshikawa et al ., 2013; Nakajima et al ., 2014).…”