Cloudberry (Rubus chamaemorus) cell culture with bioactive substances: Establishment and mass propagation for industrial use

Abstract

The availability and quality of wild berry material is strongly affected by seasonal variations, human resources, and both chemical and biological pollutants. Here we describe for the first time the industrial scale biotechnological production of cloudberry (Rubus chamaemorus) cells of consistent quality and defined chemical composition. Callus was initiated from sterile cuts of the wild cloudberry leaves. Bright yellow, soft callus of uniform quality was selected by regular subculturing, and a stable line successfully used for suspension culture was obtained within 18 months. The cultivation process of the cell culture was initiated in 250 mL shake flasks and stepwise expanded to a stirred tank bioreactor of 300 L working volume. Each growth period in flasks and fed-batch cultures in bioreactors was 10 ± 3 days. Viability of the cultures was approximately 90% throughout the cultivation processes. The 300 L culture was harvested by pressure filtration and the biomass was freeze-dried. The 300 L yields of fresh cells and freeze-dried material were 19 and 2 kg, respectively. The unusual phenolic profile of cloudberry cells including flavanols, as well as their fatty acid composition with a high proportion of α-linolenic acid and high protein content, make them a unique and interesting alternative for industrial applications for various industrial fields.

Comparison of the Micromorphology and Ultrastructure of Pollen Grains of Selected Rubus idaeus L. Cultivars Grown in Commercial Plantation

“…Given the commercial production of its fruit, Rubus idaeus has considerable economic importance in Europe and Poland [ 22 , 23 , 24 , 25 , 26 , 27 ]. Raspberry fruit is intended either for direct consumption or for processing in many industries: food [ 20 , 28 , 29 , 30 , 31 ], pharmaceutical [ 20 , 28 , 32 , 33 ], cosmetic [ 28 , 30 , 33 , 34 , 35 , 36 ], and nanotechnological [ 37 , 38 , 39 , 40 ] industries. Raspberry shrubs are a source of medicinal and cosmetic raw materials, i.e., Rubi idaeus fructus [ 41 , 42 , 43 , 44 , 45 ] and Rubi idaeus folium [ 20 , 46 , 47 , 48 ].…”

Bioconversion to Raspberry Ketone is Achieved by Several Non-related Plant Cell Cultures

“…Hordeum vulgare ‘Pokko’ cell suspension culture (barley, VTTCC P-120080) was established and maintained as described in Ritala et al (1993) . Rubus idaeus (raspberry, VTTCC P-120090), Rubus chamaemorus (cloudberry, VTTCC P-120083, Supplementary Figure S2 ) and Rubus arcticus (arctic bramble, VTTCC P120089) were maintained as described in Nohynek et al (2014) with slight modifications ( Supplementary Figure S5 ). Vaccinium myrtillus (bilberry, VTTCC P-120045) cell suspension was maintained in modified McCown Woody Plant medium ( McCown and Lloyd, 1981 ).…”

Anti-Aging Properties of Plant Stem Cell Extracts

“…motia) [39]. Very high concentrations of kinetin were found in stem cells of lemon (Citrus limon) [41] and raspberry (Rubus chamaemorus) [42]. Kinetin is considered to be a strong natural antioxidant which protects protein and nucleic acids from oxidation and glycoxidation processes [43][44][45].…”