Cloud point extraction-HPLC method for determination and pharmacokinetic study of flurbiprofen in rat plasma after oral and transdermal administration

Han, Fei; Yin, Ran; Shi, Xiaolei; Jia, Qiang; Liu, Hong-zhuo; Yao, Huimin; Xu, Lu; Li, Sanming

doi:10.1016/j.jchromb.2008.04.024

Cited by 48 publications

(16 citation statements)

References 37 publications

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“…Although CPE based on non-ionic Triton X-114 surfactant can be performed at around 25°C (cloud-point temperature of Triton X-114 is 23°C) (Santalad et al 2009). Theoretically, the optimal equilibration temperature for the extraction occurs when temperature is 15-20°C higher than the cloud-point of surfactant (Han et al 2008;Wang et al 2006). In this study, temperature was studied in the range of 35-55°C (see in Fig.…”

Section: Effect Of Equilibrium Temperature and Incubation Timementioning

confidence: 99%

Cloud-point extraction and reversed-phase high performance liquid chromatography for analysis of phenolic compounds and their antioxidant activity in Thai local wines

et al. 2011

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A cloud-point extraction (CPE) was developed for the determination of 12 phenolic compounds (i.e. gallic acid, procatechuic acid, p-hydroxybenzoic acid, vanillic acid, caffeic acid, syringic acid, p-coumaric acid, ferulic acid, guaiacol, p-cresol, o-cresol and 3,5-xylenol) using reversed-phase high performance liquid chromatography (RP-HPLC) with photodiode array (PDA) detection. The optimum CPE conditions were 2.0% (w/v) Triton X-114, 3.0% (w/v) Na2SO4 and 20-min equilibrated at 45 °C. The surfactant-rich phase was then analyzed by HPLC using a Symmetry C18 column, gradient mobile phase of acetonitrile and 1% (v/v) acetic acid, and PDA detection at 280 nm. Under the optimum condition, the target phenolic compounds were separated within 25 min. CPE gave higher enrichment factor up to 15-fold compared to that of direct analysis. The proposed method showed good analytical performances with limits of detection in the range 0.01-0.1 mg L(-1) and precisions with relative standard deviation (RSD) lower than 5% for retention time and 10% for peak area. The method was successfully applied to the analysis of phenolic compounds in Thai local wine samples. Gallic acid, procatechuic acid, and vanillic acid were the highest phenolics found in the studied wines with the contents up to 172.4, 99.1, and 26.6 mg L(-1), respectively. The recovery of the spiked wine samples (0.5, 1.0, and 2.0 mg L(-1)) were obtained in the range of 90.4-110%. High total phenolic content, total flavonoids, and antioxidant activity (DPPH method) in the studied wines were also observed.

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Section: Effect Of Equilibrium Temperature and Incubation Timementioning

confidence: 99%

Cloud-point extraction and reversed-phase high performance liquid chromatography for analysis of phenolic compounds and their antioxidant activity in Thai local wines

et al. 2011

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“…are the typical surfactants used in the CPE processes, . However, these surfactants possess alkyl phenyl groups in their hydrophobic moiety, which may attract some environmental concerns . Therefore, biodegradable surfactants such as ethoxylated alcohols without phenyl groups and branch alkyl chains are proposed.…”

Section: Resultsmentioning

confidence: 99%

Determination of strychnine, brucine, strychnine N‐oxide, and brucine N‐oxide in plasma samples after the oral administration of processed semen strychni extract by high‐performance liquid chromatography with ultrasound‐assisted mixed cloud point extraction

Guo

Meng

et al. 2016

J of Separation Science

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A sensitive and efficient mixed cloud point extraction combined with high-performance liquid chromatography was developed for the simultaneous separation and determination of four alkaloids (strychnine, strychnine N-oxide, brucine, and brucine N-oxide) in plasma after the oral administration of processed semen strychni extract. Tergitol TMN-6 and cetyl-trimethyl ammonium bromide were chosen as the mixed surfactants, and ultrasound was employed to enhance the extraction efficiency. Some important parameters affecting the mixed cloud point extraction efficiency, such as the content of Tergitol TMN-6 and cetyl-trimethyl ammonium bromide, pH, salt effect, extraction temperature, and ultrasound time were studied and optimized. Under optimum conditions, the linear range of four alkaloids was from 1.0 to 1000 ng/mL. All correlation coefficients of the calibration curves were higher than 0.9993. The intraday and interday precision were below 8.65% and the limits of detection for the four alkaloids were less than 1.0 ng/mL (S/N = 3).

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“…The type of surfactant used is also very important. Triton X-114 is more frequently chosen for the analysis of biological fluids than other surfactants such as Triton X-100 and Genapol X-080 [14][15][16][17][18][19][20][21][22][23]. This is due to its low cloud-point temperature (23°C) and high density which facilitate phase separation by centrifugation [14].…”

Section: Optimization Of the Cpe Proceduresmentioning

confidence: 99%

“…For organic molecules, especially ionizable ones, the maximum extraction efficiency is achieved at those pH values where the uncharged form of the analyte prevails and, therefore, the analyte is favored to be partitioned into the micellar phase [19]. During the experiment the NaOH concentrations were tested in the range of 0.05-0.5 M corresponding to a concentration of 0.02-0.2 M in the extraction mixture.…”

Section: Effect Of the Naoh Concentrationmentioning

confidence: 99%

Cloud-point extraction is compatible with liquid chromatography coupled to electrospray ionization mass spectrometry for the determination of antazoline in human plasma

Giebułtowicz

Kojro

Piotrowski

et al. 2016

Journal of Pharmaceutical and Biomedical Analysis

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Cloud point extraction-HPLC method for determination and pharmacokinetic study of flurbiprofen in rat plasma after oral and transdermal administration

Cited by 48 publications

References 37 publications

Cloud-point extraction and reversed-phase high performance liquid chromatography for analysis of phenolic compounds and their antioxidant activity in Thai local wines

Cloud-point extraction and reversed-phase high performance liquid chromatography for analysis of phenolic compounds and their antioxidant activity in Thai local wines

Determination of strychnine, brucine, strychnine N‐oxide, and brucine N‐oxide in plasma samples after the oral administration of processed semen strychni extract by high‐performance liquid chromatography with ultrasound‐assisted mixed cloud point extraction

Cloud-point extraction is compatible with liquid chromatography coupled to electrospray ionization mass spectrometry for the determination of antazoline in human plasma

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