Cloud point extraction–HPLC method for the determination and pharmacokinetic study of aristolochic acids in rat plasma after oral administration of Aristolochiae Fructus

Ren, Gang; Huang, Qun; Wu, Jiangang; Jie, Yuan; Yang, Gaihong; Yan, Zhihong; Yao, Shouzhuo

doi:10.1016/j.jchromb.2014.01.055

Cited by 18 publications

(7 citation statements)

References 39 publications

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“…When rats orally administrated with 8 mg/kg AAI, the C max of AAI was 1352.8 ng/mL (Liu et al, 2010). When rats orally administrated with 30 g/kg Aristolochiae Fructus extract, the C max of AAI was about 2056.6 ng/mL (Ren et al, 2014). Intestinal tract is the main site of food digestion and absorption, and also the main site of drug absorption.…”

Section: Introductionmentioning

confidence: 99%

Aristolochic acid I is a substrate of BCRP but not P-glycoprotein or MRP2

Qin

Shen

et al. 2015

Journal of Ethnopharmacology

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Section: Introductionmentioning

confidence: 99%

Aristolochic acid I is a substrate of BCRP but not P-glycoprotein or MRP2

Qin

Shen

et al. 2015

Journal of Ethnopharmacology

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“…Five AAs, shown in Fig. 1 , were separated and purified in our laboratory and their purities were no lower than 98% [ 13 , 26 ]. Methanol and acetonitrile were HPLC grade (Fairfield, OH, USA).…”

Section: Methodsmentioning

confidence: 99%

“…Nowadays, the pharmacokinetic studies about AAs focus mainly on AA I [ 17 – 19 ] and/or AA II [ 20 – 25 ]; the other AAs are seldom involved. Very recently, the preliminary pharmacokinetics of 4 AAs in AF have been investigated by the CPE -HPLC (cloud point extraction-high performance liquid chromatography) method [ 26 ]. However, the method is tedious and produces data with relatively poor sensitivity.…”

Section: Introductionmentioning

confidence: 99%

“…Advances in sample pretreatment and analytical techniques have improved analysis time, sensitivity and efficiency. A number of chromatography methods have been reportedly used for the determination of AAs in herbal plants [ 9 – 15 ] and biological samples [ 26 – 30 ]. Among these, high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) has been employed for the quantitative analysis of AA I [ 19 ] and AA II [ 22 , 29 ] in biological matrices.…”

Section: Introductionmentioning

confidence: 99%

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Comparative studies on the multi-component pharmacokinetics of Aristolochiae Fructus and honey-fried Aristolochiae Fructus extracts after oral administration in rats

Jie

Ren

Liang

et al. 2017

BMC Complement Altern Med

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Background Aristolochiae Fructus (AF) and honey-fried Aristolochiae Fructus (HAF) have been used in China for a long time as anti-tussive and expectorant drugs. Few clinical cases have been reported to be associated with the toxicity of AF and HAF, although relatively high amounts of aristolochic acids (AAs) have been found in them. Our previous experiments have verified from the chemical changes and from traditional toxicology that honey-processing can significantly reduce the toxicity of AF. To further elucidate the detoxification mechanism of honey-processing, comparative pharmacokinetics of AAs in AF and HAF are performed in this study.MethodsAn HPLC-MS/MS (high-performance liquid chromatography-tandem mass spectrometry) method was developed and validated for the determination of AA I, AA II, AA C, AA D and 7-OH AA I in rat plasma. The multi-component pharmacokinetics of AAs in AF and HAF extracts were investigated after the oral administration of three doses to rats. The relative pharmacokinetic parameters were compared systematically.ResultsThe five AAs shared a similar nonlinear PK (pharmacokinetic) process. They involve rapid absorption and elimination, and they were fit into a two-compartmental open model. Some significant pharmacokinetic differences were observed between the AF and HAF groups: the C max and AUC values of AA I and AA II in the AF groups were much higher than those of the HAF groups.ConclusionsHoney-frying technology can reduce the toxicity of AF by significantly decreasing the absorption of AA I and AA II. The PK parameters obtained in this work could provide valuable references for the toxicity research and clinical use of Aristolochiaceae herbs, including AF and HAF.Graphical abstractProcess diagram of comparative pharmacokinetics study Electronic supplementary materialThe online version of this article (doi:10.1186/s12906-017-1626-2) contains supplementary material, which is available to authorized users.

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“…The type of surfactant used is also very important. Triton X-114 is more frequently chosen for the analysis of biological fluids than other surfactants such as Triton X-100 and Genapol X-080 [14][15][16][17][18][19][20][21][22][23]. This is due to its low cloud-point temperature (23°C) and high density which facilitate phase separation by centrifugation [14].…”

Section: Optimization Of the Cpe Proceduresmentioning

confidence: 99%