Clotrimazole enhances lysis of human erythrocytes induced by t-BHP

Lisovskaya, I. L.; Shcherbachenko, Irina M.; Volkova, R. I.; Ataullakhanov, F. I.

doi:10.1016/j.cbi.2009.04.003

Cited by 21 publications

(19 citation statements)

References 47 publications

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“…Accordingly, it is reasonable to conclude that both extracts from this plant protects erythrocytes from lipid damage cased by t-BHP. On the other hand, our results allow us to also suggest that both extracts enhances t-BHPinduced hemolysis by forming a complex(es) with cell components released during damage mediated by t-BHP, which are believed to destabilizes the cell membrane, accordingly to previously described (Huy et al 2002, Lisovskaya et al 2009). …”

supporting

confidence: 86%

Mentha pulegium crude extracts induce thiol oxidation and potentiate hemolysis when associated to t-butyl hydroperoxide in human’s erythrocytes

Bianchini

Galvão

Tamborena

et al. 2017

An. Acad. Bras. Ciênc.

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Mentha pulegium (Lamiaceae) tea has been used as a traditional medicine; however, the modulatory effect of M. pulegium extracts on damage to human erythrocytes associated to t-butyl hydroperoxide (t-BHP) exposure remains to be investigated. Accordingly, we perform this study in order to test the hypothesis that aqueous and ethanolic extracts of M. pulegium could modulate the hemolysis associated to t-BHP exposure, non-protein thiol (NPSH) oxidation and lipid peroxidation (measured as thiobarbituric acid reactive substances -TBARS) in human erythrocytes. Samples were co-incubated with t-BHP (4 mmol/L) and/or aqueous or ethanolic extracts (10-1000 mg/mL) during 120 min to further analysis. We found that both extracts, when associated to t-BHP, potentiate NPSH oxidation and hemolysis. Moreover, both extracts significantly prevents against t-BHP-induced TBARS production. A significant correlation among hemolysis and NPSH levels was found. Taking together, our data points that the association of M. pulegium extracts with t-BHP culminates in toxic effect to exposed erythrocytes, besides its protective effect against t-BHP-induced TBARS production. So, we infer that the use of this extract may exert negative effect during painful crisis in sickle cell anemia. However, more studies are still necessary to better investigate/ understand the mechanism(s) involved in the toxic effect resultant from this association.

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supporting

confidence: 86%

Mentha pulegium crude extracts induce thiol oxidation and potentiate hemolysis when associated to t-butyl hydroperoxide in human’s erythrocytes

Bianchini

Galvão

Tamborena

et al. 2017

An. Acad. Bras. Ciênc.

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“…A second requirement for the use of RBCs as antimalarial carriers is that when present at therapeutically active concentration, the drug has to be innocuous for the cell physiology, which might not be an unsurmountable obstacle given the reduced metabolic activity of erythrocytes. However, loading of some antimalarial drugs like clotrimazole had been observed to predispose RBCs to oxidative damage [65], an undesirable scenario since oxidized RBCs are rapidly taken up by hepatic reticuloendothelial system macrophages. Finally, because LPs adsorbed on RBC surfaces would probably sufficiently modify cell shape to target it for removal through spleen filtration, a compromise between stable drug containment and lipid bilayer fusion might have to be reached through the adequate LP formulation, with the objective of achieving LP-RBC merging before spleen removal while avoiding rapid drug leaking from LPs.…”

Section: Discussionmentioning

confidence: 99%

Immunoliposome-mediated drug delivery to Plasmodium -infected and non-infected red blood cells as a dual therapeutic/prophylactic antimalarial strategy

Moles

Urbán

Jiménez-Dı́az

et al. 2015

Journal of Controlled Release

112

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“…However, loading of some antimalarial drugs like clotrimazole had been observed to predispose RBCs to oxidative damage [11], an undesirable scenario because oxidized RBCs are rapidly taken up by hepatic reticuloendothelial system macrophages.…”

Section: Which Are the Limitations Of Erythrocytes As Drug Carriers?mentioning

confidence: 99%

Loading Antimalarial Drugs into Noninfected Red Blood Cells: An Undesirable Roommate for Plasmodium

Moles

Fernàndez‐Busquets

2015

Future Med. Chem.

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Clotrimazole enhances lysis of human erythrocytes induced by t-BHP

Cited by 21 publications

References 47 publications

Mentha pulegium crude extracts induce thiol oxidation and potentiate hemolysis when associated to t-butyl hydroperoxide in human’s erythrocytes

Mentha pulegium crude extracts induce thiol oxidation and potentiate hemolysis when associated to t-butyl hydroperoxide in human’s erythrocytes

Immunoliposome-mediated drug delivery to Plasmodium -infected and non-infected red blood cells as a dual therapeutic/prophylactic antimalarial strategy

Loading Antimalarial Drugs into Noninfected Red Blood Cells: An Undesirable Roommate for Plasmodium

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