Clostridium perfringens: A Dynamic Foodborne Pathogen

García, Santos; Heredia, Norma

doi:10.1007/s11947-009-0182-2

Cited by 39 publications

(21 citation statements)

References 35 publications

(43 reference statements)

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“…The organism was not isolated from any of the frozen meat and two isolations were made from the refrigerated meat samples only. This finding supports the assumption that food samples tested for the presence of C. perfringens vegetative cells should be analyzed immediately without being frozen (García and Heredia 2011). …”

Section: Discussionsupporting

confidence: 82%

“…Selected isolates were further characterized through their biochemical profile like double haemolysis on blood agar (García and Heredia 2011), reverse Christie Atkins Munch-Petersen (CAMP) test (Brady et al 2010), lecithinase and proteolytic activity as well as gelatin liquefaction, nitrate reduction and lactose fermentation tests (Lindstrom et al 2011;Skariyachan et al 2010). …”

Section: Identification Of Microorganismmentioning

confidence: 99%

“…Clostridium perfringens is a gram positive, rod shaped, spore forming, anaerobic bacteria (Bergey 2009;Quinn et al 2002) which is a common inhabitant of soil and gastrointestinal tract of humans and animals (García and Heredia 2011;Li et al 2007). Apart from causing enteric and systemic infections in animals and poultry, C. perfringens is responsible for food poisoning cases in humans worldwide (Skariyachan et al 2010).…”

Section: Introductionmentioning

confidence: 99%

“…Anaerobic environment of the intestinal lumen allows the survivors to multiply and sporulate. Production of a specific type of active enterotoxin causes characteristic symptoms of C. perfringens food poisoning (García and Heredia 2011). C. perfringens presence in meat products is a the major concern in the preparation of high quality foods (Abostate et al 2006).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Toxinotyping and antimicrobial susceptibility of enterotoxigenic Clostridium perfringens isolates from mutton, beef and chicken meat

et al. 2014

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A total of 300 meat samples comprising mutton, beef, and chicken meat (n=100) collected from either local butcher shops or large meat outlets situated at various areas of Lahore City located in Punjab province of Pakistan were tested for the isolation of Clostridium perfringens. Prevalence of the organism was highest in the chicken (6 %) followed by mutton (5 %) and beef (1 %). Contamination level was high (10/150) in the samples collected from local butcher shops in comparison to the samples collected from large meat outlets (2/150). All of the raw meat samples were negative for the presence of alpha, beta and epsilon toxins of C. perfringens as detected through ELISA. Out of a total number of 12 isolates only half were capable of producing enterotoxins when cultured in trypticase glucose yeast (TGY) broth. Toxinotyping of the isolates showed that 3 were of type A while one each of the remaining three belonged to type B, C, and D. Antibiotic susceptibility testing of the toxin producing isolates revealed that C. perfringens were susceptible to chloramphenicol, ciprofloxacin, metronidazole, and ceftriaxone. All of the other drugs were relatively less effective with a least activity of amoxicillin against the isolates.

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Section: Discussionsupporting

confidence: 82%

Section: Identification Of Microorganismmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Toxinotyping and antimicrobial susceptibility of enterotoxigenic Clostridium perfringens isolates from mutton, beef and chicken meat

et al. 2014

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“…Although this method can greatly reduce the time consumption, which is still much longer and sensitive to temperature. Some biological methods, such as polymerase chain reaction (PCR) and enzyme linked immunosorbent assay (ELISA) have also been developed and applied for microbial identification and determination [5,6]. Although the results are relatively accurate and reliable, they are also accompanied with strong technicality and high cost.…”

Section: Introductionmentioning

confidence: 99%

A Nondestructive Real-Time Detection Method of Total Viable Count in Pork by Hyperspectral Imaging Technique

2017

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A nondestructive method was developed for assessing total viable count (TVC) in pork during refrigerated storage by using hyperspectral imaging technique in this study. The hyperspectral images in the visible/near-infrared (VIS/NIR) region of 400-1100 nm were acquired for fifty pork samples, and their VIS/NIR diffuse reflectance spectra were extracted from the images. The reference values of TVC in pork samples were determined by classical microbiological plating method. Both partial least square regression (PLSR) model and support vector machine regression model (SVR) of TVC were built for comparative analysis to achieve better results. Different transformation methods and filtering methods were applied to improve the models. The results show that both the optimized PLSR model and SVR model can predict the TVC very well, while the SVR model based on second derivation was better, which achieved with R P (correlation coefficient of prediction set) = 0.94 and SEP (standard error of prediction set) = 0.4570 log CFU/g in the prediction set. An image processing algorithm was then developed to transfer the prediction model to every pixel of the image of the entire sample; the visualizing map of TVC would be displayed in real-time during the detection process due to the simplicity of the model. The results demonstrated that hyperspectral imaging is a potential reliable approach for non-destructive and real-time prediction of TVC in pork.

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Sustainable agricultural practices contribute significantly to One Health

Yan

Xiong

Liu

et al. 2022

J of Sust Agri & Env

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The One Health concept proposes that the health of humans, animals, and the environment are interconnected. Agricultural production is a critical component of One Health as food links the environment to human health. Food not only provides nutrients to humans but also represents an important pathway for human exposure to environmental microbes as well as potentially harmful agrochemicals. In addition, inappropriate agronomic practices can cause damage to the environment which can have unintended adverse impacts on human health. Therefore, improving agricultural production systems and protecting environmental health should not be viewed as isolated goals as they are strongly interlinked. Here, we used the nexus of soil, plant, and human microbiomes to discuss sustainable agricultural production from the One Health perspective. We highlighted three interconnected challenges faced by current agronomic practices: the transmissions of pathogens in soil-human microbial loops, the dissemination of antibiotic resistance genes in agroecosystems, and the impacts of chemical pesticides on humans and environmental health. Finally, we propose the potential of utilising microbiomes for better sustainable agronomic practices to contribute to key goals of the One Health concept.

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Clostridium perfringens: A Dynamic Foodborne Pathogen

Abstract: Clostridium perfringens is a spore-forming bacterium and natural inhabitant of soil and the intestinal tracts of many warm-blooded animals, including humans. The

Cited by 39 publications

References 35 publications

Toxinotyping and antimicrobial susceptibility of enterotoxigenic Clostridium perfringens isolates from mutton, beef and chicken meat

Toxinotyping and antimicrobial susceptibility of enterotoxigenic Clostridium perfringens isolates from mutton, beef and chicken meat

A Nondestructive Real-Time Detection Method of Total Viable Count in Pork by Hyperspectral Imaging Technique

Sustainable agricultural practices contribute significantly to One Health

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