Clostridium neopropionicum sp. nov., a strict anaerobic bacterium fermenting ethanol to propionate through acrylate pathway

Tholozan, Jean-Luc; Touzel, Jean Pierre; Samain, E.; Grivet, Jean‐Philippe; Prensier, G.; Albagnac, Guy

doi:10.1007/bf00245158

Cited by 71 publications

(98 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Although acrylate utilization by M. elsdenii and other organisms using the acrylyl-CoA pathway has been established, the details of acrylate assimilation have not been clarified. Most investigators propose direct assimilation to acrylyl-CoA via an acrylyl-CoA synthetase (12,29). Our observations cast doubt on the presence of an acrylyl-CoA synthetase, instead pointing to the assimilation of acrylate and analogues via the general CoA transferase.…”

Section: Discussioncontrasting

confidence: 47%

Lactate and Acrylate Metabolism by Megasphaera elsdenii under Batch and Steady-State Conditions

Prabhu

Altman

Eiteman

2012

Appl Environ Microbiol

140

View full text Add to dashboard Cite

The growth of Megasphaera elsdenii on lactate with acrylate and acrylate analogues was studied under batch and steady-state conditions. Under batch conditions, lactate was converted to acetate and propionate, and acrylate was converted into propionate. Acrylate analogues 2-methyl propenoate and 3-butenoate containing a terminal double bond were similarly converted into their respective saturated acids (isobutyrate and butyrate), while crotonate and lactate analogues 3-hydroxybutyrate and (R)-2-hydroxybutyrate were not metabolized. Under carbon-limited steady-state conditions, lactate was converted to acetate and butyrate with no propionate formed. As the acrylate concentration in the feed was increased, butyrate and hydrogen formation decreased and propionate was increasingly generated, while the calculated ATP yield was unchanged. M. elsdenii metabolism differs substantially under batch and steady-state conditions. The results support the conclusion that propionate is not formed during lactate-limited steady-state growth because of the absence of this substrate to drive the formation of lactyl coenzyme A (CoA) via propionyl-CoA transferase. Acrylate and acrylate analogues are reduced under both batch and steady-state growth conditions after first being converted to thioesters via propionyl-CoA transferase. Our findings demonstrate the central role that CoA transferase activity plays in the utilization of acids by M. elsdenii and allows us to propose a modified acrylate pathway for M. elsdenii. Megasphaera elsdenii is an ecologically important rumen bacterium whose genome has recently been sequenced (20) and which metabolizes DL-lactate principally to propionate and acetate (5,7,19). Lactate conversion to propionate occurs via the acrylate pathway with acrylyl coenzyme A (CoA) serving as an intermediate (32), a pathway also used by several other organisms, including Clostridium propionicum. The dead-end reduction of lactate to propionate allows the cell to balance the anaerobic oxidation of lactate to acetate and carbon dioxide (16), steps which appear to be the primary means of ATP generation (26). M. elsdenii also produces butyrate, and several strains also accumulate longer-chain fatty acids from the fermentation of lactate (9). The generation of butyrate from lactate relies on the presence of acetate, and M. elsdenii also has the flexibility to generate hydrogen from reduced ferredoxin as another means to balance redox (11). While M. elsdenii continues to be of great interest as a member of the rumen microbial community (21, 31), the organism and its enzymes also have potential biotechnological applications (28).Key steps in the metabolic pathway of M. elsdenii reduction of lactate are mediated by propionyl-CoA transferase (24), lactylCoA dehydratase (2, 14, 17), and acrylyl-CoA reductase (3, 10). Propionyl-CoA transferase (EC 2.8.3.1; systematic name, acetylCoA:propionate-CoA-transferase) is typically implicated in the interconversion of propionate/propionyl-CoA and DL-lactate/DLlactyl-CoA (26). However, th...

show abstract

Section: Discussioncontrasting

confidence: 47%

Lactate and Acrylate Metabolism by Megasphaera elsdenii under Batch and Steady-State Conditions

Prabhu

Altman

Eiteman

2012

Appl Environ Microbiol

140

View full text Add to dashboard Cite

show abstract

“…The initial concentration of ethanol was 30 mM (1.38 g*L -1 ) and the produced propionic acid concentration was 1.25 g*L -1 . Also the second and third highest values were achieved in this report [35].…”

Section: Propionic Acid Yieldsupporting

confidence: 50%

“…In Table 2 the highest propionic acid yield is 0.90 g*g -1 [35]. This was achieved with Clostridium neopropionicum which was isolated from a mesophilic anaerobic digester treating industrial vegetable cannery wastewater.…”

Section: Propionic Acid Yieldmentioning

confidence: 99%

Bio-produced Propionic Acid: A Review

Vidra

Németh

2017

Period. Polytech. Chem. Eng.

View full text Add to dashboard Cite

show abstract

“…A propionic fermentation is carried out by a wide variety of bacteria belonging to various phylogenetic groups. Examples are the high-G + C Gram-positive genus Propionibacterium (Olsen et al, 1994), some representatives of the Clostridiurn subphylum (see Tholozan et al, 1992;Evers et al, 1993;Collins et al, 1994, for example), certain rod-shaped species of the &subclass of the Proteobacteria (Schink, 1984), coccoid and curved bacteria which belong to cluster IX of the Gram-positive anaerobes such as Veillonella, Selenomonas and Pectinatus (Collins et al, 1994 ;Willems & Collins, 1999, and Gram-negative vibrioid or spirilloid bacteria of unknown phylogenetic position such as Anaerouibrio (Prins, 1984) and Propionispira (Schink et al, 1982). A propionic fermentation with succinate as an additional product was recently reported for oxygen-tolerant saccharolytic ultramicrobacteria from rice paddy soil belonging to the Verrucomicrobiales (Janssen et al, 1997).…”

Section: Discussionmentioning

confidence: 99%

Fermentative bacteria from estuarine mud: Phylogenetic position of Acidaminobacter hydrogenoformans and description of a new type of Gram-negative, propionigenic bacterium as Propionibacter pelophilus gen. nov., sp. nov.

Meijer

Nienhuis-Kuiper

Hansen

1999

International Journal of Systematic and Evolutionary Microbiology

View full text Add to dashboard Cite

The phylogenetic positions of t w o strains of fermentative bacteria that had been isolated from the highest positive tubes inoculated with serial dilutions of estuarine mud in agar media with either glutamate or aspartate as substrate were determined by comparative sequence analysis of their 16s rRNA genes. The strain isolated with glutamate (glu 65) utilized several substrates, including a number of amino acids but no sugars. The degradation of certain substrates was enhanced by or dependent upon co-cultivation with a hydrogen-utilizing partner. In earlier work this strain was assigned t o the new genus and species Acidaminobacter hydrogenoformans. On the basis of i t s 165 rRNA gene sequence Acidaminobacter hydrogenoformans has now been identified as a member of cluster XI of the Clostridium subphylum with Clostridium halophilum as its closest relative. The aspartate-fermenting strain asp 66l was a Gram-negative, rather aerotolerant anaerobe which utilized a wide range of substrates in a propionic fermentation and had the ability to fix molecular nitrogen. Strain asp 66T was shown to be a new member of the j?-subclass of the Proteobacteria with Azoarcus sp. strain 6a3 and Rhodocyclus tenuis as i t s closest relatives. It is described as Propionibacter pelophilus gen. nov., sp. nov., with the type strain asp 66l(= DSM 120183. 1

show abstract

Clostridium neopropionicum sp. nov., a strict anaerobic bacterium fermenting ethanol to propionate through acrylate pathway

Cited by 71 publications

References 43 publications

Lactate and Acrylate Metabolism by Megasphaera elsdenii under Batch and Steady-State Conditions

Lactate and Acrylate Metabolism by Megasphaera elsdenii under Batch and Steady-State Conditions

Bio-produced Propionic Acid: A Review

Fermentative bacteria from estuarine mud: Phylogenetic position of Acidaminobacter hydrogenoformans and description of a new type of Gram-negative, propionigenic bacterium as Propionibacter pelophilus gen. nov., sp. nov.

Contact Info

Product

Resources

About