Clostridium fessum sp. nov., isolated from human faeces

Abstract

An obligately anaerobic, Gram-stain-positive and spore-forming strain, SNUG30386T was isolated from a faecal sample of a healthy Korean subject. The strain formed a round ivory-coloured colony and cells were chained rods with tapered ends, approximately 2.0–2.5×0.6–0.8 μm in size. The taxonomic analysis indicated that strain SNUG30386T was within the family Lachnospiraceae . According to the 16S rRNA gene sequence similarity, the closest species to strain SNUG30386T was Clostridium symbiosum (95.6 %), followed by Enterocloster asparagiformis (94.8 %), Enterocloster clostridioformis (94.8 %) and Enterocloster lavalensis (94.6 %). The evolutionary tree based on 16S rRNA gene sequences demonstrated that strain SNUG30386T had split apart at a unique branch point far from other close relatives. Its DNA G+C content was 48.3 mol% calculated from the whole genome sequence. The major cellular fatty acids were C16 : 0 and C14 : 0. Compared to those of the closely related species, strain SNUG30386T showed distinct biochemical activities such as being unable to utilize most of carbon sources except d-glucose and l-arabinose. As a result, based on its unique phylogenetic clade and taxonomic characteristics, we conclude that strain SNUG30386T represents a novel species within the genus Clostridium , for which the name Clostridium fessum sp. nov. is proposed. The type strain of the novel species is SNUG30386T (=KCTC 15633T= JCM 32258T).

Enterocloster alcoholdehydrogenati sp. nov., a Novel Bacterial Species Isolated from the Feces of a Patient with Alcoholism

“…1 ). The results showed that C5-48 T co-clustered with C. fessum SNUG30386 T [ 28 ] and L. edouardi Marseille-P3397 T [ 29 ], and this cluster was closely related to the Enterocloster cluster although these two strains were not previously considered to be strains of Enterocloster [ 8 ]. To further confirm the phylogenetic position of strain C5-48 T , we performed phylogenetic analysis based on the nucleotide sequences coding for the β subunit of RNA polymerase ( rpoB ) (see Fig.…”

Clostridium folliculivorans sp. nov., isolated from soil samples of an organic paddy in Japan

“…The fatty acid type such as C 14 : 0 or C *Data for C. manihotivorum CT4 T taken from [16]. [22,[63][64][65][66][67][68][69][70]. Characteristically, the fatty acids C 12 : 0 FAME, summed feature 1 and summed feature 6 were present in strain D1-1 T , but were absent in the type strains of the most closely related species C. zeae.…”

“…thailandense , and C. beihaiense [22, 63–70]. Characteristically, the fatty acids C 12 : 0 FAME, summed feature 1 and summed feature 6 were present in strain D1-1 T , but were absent in the type strains of the most closely related species C.…”

Faecalicatena absiana sp. nov., an obligately anaerobic bacterium from a pig farm faeces dump

“…Recently renewed interest in the gut microbiome has led to the isolation and description of several new species and an increase in the composition of Lachnospiraceae taxa by ~50 % [7]. Members of the Clostridium cluster XIVa belonging to the family Lachnospiraceae have been classified into new genera such as Faecalicatena , Dorea , Coprococcus , Hungatella , Enterocloster , Lacrimispora , Anaerotignum , Roseburia , Butyrivibrio , Mediterraneibacter and Faecalimonas [8, 9]. Among these genera, a few species from the genus Faecalicatena were reclassified as Faecalicatena contorta , Faecalicatena fissicatena and Faecalicatena orotica [10].…”