Clostridium butyricum improves the intestinal health of goats by regulating the intestinal microbial community

Zhang, Chengrui; Hou, Tingyi; Yu, Qingyuan; Wang, Jihong; Ni, Miao; Ye, Zi; Xin, Hangshu; Zhang, Yonggen; Sun, Yukun

doi:10.3389/fmicb.2022.991266

Cited by 7 publications

(10 citation statements)

References 42 publications

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“…These results showed that adding C. butyrate to the diet could block the adhesion of Eubacterium]_xylanophilum_group and Lachnospiraceae_UCG-001 in the intestinal tract of goats and inhibit their activity. At the same time, the correlation between the decline of Clostridium and Kineothrix relative abundance and TNF-α , T-SOD were consistent with previous studies in the cecum ( 17 ). It indicated that dietary supplementation of C. butyricum could effectively reduce the relative abundance of harmful Clostridium in the intestine of goats.…”

Section: Discussionsupporting

confidence: 91%

“…The amplicon pools were prepared for sequencing while the size and quantity of the amplicon library were assessed on Agilent 2100 Bioanalyzer (Agilent, USA) and with the Library Quantification Kit for Illumina (Kapa Biosciences, Woburn, MA, USA), respectively. The libraries were sequenced on the NovaSeq PE250 platform ( 17 ).…”

Section: Methodsmentioning

confidence: 99%

“…The correlation heatmap was analyzed through the Spearman correlation matrix, which was also drawn by the R package (v3.6.3). Other graphs were implemented using the R package (v3.5.2) ( 17 ).…”

Section: Methodsmentioning

confidence: 99%

“…Following the manufacturer's instructions, DNA from feces was extracted using the E.Z.N.A .® Stool DNA Kit (D4015, Omega, Inc., The libraries were sequenced on the NovaSeq PE250 platform (17). 16S rDNA were sequenced on the Illumina NovaSeq platform following the manufacturer's recommendations provided by LC-Bio.…”

Section: Microbial Compositionmentioning

confidence: 99%

“…Our previous trial’s results showed that high-concentrate dietary supplementation with C. butyricum could influence the production and transport of volatile fatty acid in the rumen by regulating the structure of rumen microbiota to stabilize the rumen pH and improving the persistent effect of feeding high-concentrate diets on the rumen of fattening goats in the short term ( 16 ). In the meantime, we found that C. butyricum could reach the intestine through the rumen and enhanced the intestinal epithelial barrier function of goats by regulating the intestinal microbial community ( 17 ). The intestinal barrier is a complex defense barrier formed by intestinal epithelial cells, mucus layer, intestinal microbiota, secreted antibodies, and other substances.…”

Section: Introductionmentioning

confidence: 99%

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Clostridium butyricum alleviates LPS-induced acute immune stress in goats by regulating bacterial communities and blood metabolites

et al. 2023

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The mitigation and prevention of acute immune stress are essential for livestock production. Clostridium butyricum (C. butyricum) has shown positive effects in stabilizing intestinal microbiota disorders, improving immune function and inhibiting disease development, but its effects on ruminants are unclear. Therefore, the current trial hypothesized that C. butyricum could improve goats’ immune function and antioxidant capacity by regulating bacterial communities and blood metabolism and effectively alleviating the acute immune stress induced by Lipopolysaccharides (LPS). Sixteen healthy goats were fed C. butyricum for 70 days, and the goats were challenged with LPS on day 71. Blood and feces were collected at 0 h and 6 h after the challenge to evaluate the effects of C. butyricum on their intestinal microbiota, immune function, antioxidant function, and plasma metabolites. The results showed that C. butyricum had no significant effect on plasma biochemical parameters at the beginning of the LPS challenge. However, supplementation with C. butyricum increased plasma levels of IgA, IgG, T-SOD, and T-AOC (P < 0.05), but TNF-α, IL-6, and MDA were decreased (P < 0.05). In contrast, IL-10 showed an increasing trend (P < 0.10). Rectal microbiota analysis showed that C. butyricum significantly increased the relative abundance of Epsilonbacteraeota at the phylum level of goats; at the genus level, the relative abundances of Campylobacter and Anaerorhabdus]_furcosa_group were also significantly increased (P < 0.05). Christensenellaceae_R-7_group as the dominant microbiota also showed a significant increase in their abundance values, while Clostridium and Lachnospiraceae_UCG-001 were significantly lower (P < 0.05). When the LPS challenge continued up to 6 h, dietary supplementation with C. butyricum still resulted in significantly higher plasma concentrations of IgA, IL-10, and T-SOD in goats than in the control group, reducing TNF-α levels (P < 0.05). In addition, plasma levels of T-CHOL and LDL were significantly reduced, and the expression of d-proline was significantly upregulated according to metabolomic analysis (P < 0.05). In conclusion, dietary supplementation with C. butyricum helped optimize the expression of bacterial communities and plasma metabolites to enhance the ability of goats to alleviate acute immune stress.

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Section: Discussionsupporting

confidence: 91%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Microbial Compositionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Clostridium butyricum alleviates LPS-induced acute immune stress in goats by regulating bacterial communities and blood metabolites

et al. 2023

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Non‐coding RNAs and colitis‐associated cancer: Mechanisms and clinical applications

Zhao

Zheng

et al. 2023

Clinical & Translational Med

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Background Colitis‐associated cancer (CAC) is one of the most severe complications of inflammatory bowel disease (IBD), which has caused a worse survival rate in IBD patients. Although the exact aetiology and pathogenesis of CAC are not completely elucidated, evidence indicates that non‐coding RNAs are closely involved and play a key role. Methods This review aims to summarise the major findings of non‐coding RNAs in the development of CAC and present the potential mechanistic links between non‐coding RNAs and CAC pathogenesis. The results show that non‐coding RNAs can hinder DNA mismatch repair proteins and obstruct chromosome passenger complexes to increase microsatellite instability and accumulate chromosomal instability, respectively. The data also suggest that DNA promoter methylation or RNA methylation modifications of non‐coding RNA are the main mechanisms to regulate oncogene or tumour suppressor expression during the CAC progression. Other factors, including gut microbiota perturbations, immune dysregulation and barrier dysfunction, are also regulated and influenced by non‐coding RNAs. Besides, non‐coding RNAs as molecular managers are associated with multiple critical signalling pathways governing the initiation, progression and metastasis of CAC, including the janus kinase/signal transducer and activator of transcription (JAK/STAT), nuclear factor‐kappa B (NF‐κB), extracellular signal‐regulated kinase (ERK), Toll‐like receptor 4 (TLR4), Wnt/β‐catenin and phosphatidylinositol 3‐Kinase/Protein Kinase B (PI3K/AKT) pathways. In addition, non‐coding RNAs can be detected in colon tissues or blood, and their aberrant expressions and diagnostic and prognostic roles are also discussed and confirmed in CAC patients. Conclusions It is believed that a deepening understanding of non‐coding RNAs in CAC pathogenesis may prevent the progression to carcinogenesis, and will offer new effective therapies for CAC patients.

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The Potential of Clostridium butyricum to Preserve Gut Health, and to Mitigate Non-AIDS Comorbidities in People Living with HIV

Yang,

Zaongo,

Zhu

et al. 2024

Probiotics & Antimicro. Prot.

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Clostridium butyricum improves the intestinal health of goats by regulating the intestinal microbial community

Cited by 7 publications

References 42 publications

Clostridium butyricum alleviates LPS-induced acute immune stress in goats by regulating bacterial communities and blood metabolites

Clostridium butyricum alleviates LPS-induced acute immune stress in goats by regulating bacterial communities and blood metabolites

Non‐coding RNAs and colitis‐associated cancer: Mechanisms and clinical applications

The Potential of Clostridium butyricum to Preserve Gut Health, and to Mitigate Non-AIDS Comorbidities in People Living with HIV

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