Clostridium botulinum in honey: prevalence and antibiotic susceptibility of isolated strains

Koluman, Ahmet; Gölcü, Berfin Melikoğlu; Derin, Okan; ÖZKÖK, Sibel; Anniballi, Fabrizio

doi:10.3906/vet-1209-40

Cited by 9 publications

(7 citation statements)

References 15 publications

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“…For same antibiotics, the susceptibility pattern was 15(71%), and 16(76%) for isolates from local samples. In the study of Koluman et al (2013), isolates of Clostridium from 5 out of the 19 honey samples were resistant to gentamicin. However, isolates from imported samples showed 7(100%) resistance to amikacin, isolates of local samples were 9(43%) resistant to the same antibiotic.…”

Section: Resultsmentioning

confidence: 99%

Physicochemical Properties of Imported and Locally Produced Honey did not translate into its Microbial Quality and Antibacterial Activity

Nzeh

Quansah

Dufailu

2022

Preprint

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Honey is often considered a good substitute for sugar. However, concerns about the poor quality of locally produced honey have necessitated the importation of honey to meet the domestic demand in spite of Ghana’s good climatic conditions suitable for honey production. Due to the increasing level of demand for honey both locally and internationally, we highlighted here the need for an assessment of the physicochemical, microbiological quality and antibiotics resistance of microbes isolated from the imported and locally produced honey vis-à-vis international standards. Thirty honey samples which comprise 7 imported and 23 locally produced were randomly sampled from retail outlets consisting of supermarkets, marts, shops, and open markets as well as from production sites within the northern region for physicochemical analysis, microbial analysis and antibiogram. Results of the physicochemical quality analysis showed that sampled honey (both imported and locally produced) was within acceptable set standards. However, microbial quality was poor as a high incidence of bacterial contamination and resistance was recorded in the study. The High incidence of bacterial contamination and antibiotic resistance recorded in this study gives an indication that all is not well in the honey industry and therefore the need for serious concern to avert possible health issues associated with the consumption of honey found within the region.

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Section: Resultsmentioning

confidence: 99%

Physicochemical Properties of Imported and Locally Produced Honey did not translate into its Microbial Quality and Antibacterial Activity

Nzeh

Quansah

Dufailu

2022

Preprint

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“…From 69 terrestrial soil samples taken in Sweden, 20 (29%) were shown to be positive for C. botulinum type E, and 1 positive for both types E and B (Johansen, 1963). Interestingly Polish honey samples did not show the presence of C. botulinum type F which was found in honey from the Scandinavian countries (Nevas et al, 2005), Turkey (Koluman et al, 2013), and Lithuania (unpubl. data).…”

Section: Discussionmentioning

confidence: 99%

“…There is a significant variation in the prevalence of C. botulinum in European honeys. For instance, the contamination level of honey in Hungary was 17%, in Turkey it was 19%, in Denmark 14-20%, in Italy 11.8%, in Norway 10 -25%, in Spain 20%, in the UK 20%, in Germany 1-20%, in Sweden 2% (Nevas et al, 2005;Nevas, 2006;Gilbert et al, 2006;Koluman et al, 2013). The spore prevalence in Danish honey (20%) is the best example for comparing with the prevalence of C. botulinum spores in small-apiary honey from Poland (a prevalence of 21.6%), since swine production takes place in both countries.…”

Section: Discussionmentioning

confidence: 99%

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Clostridium botulinumSpores Found in Honey from Small Apiaries in Poland

Wojtacka

Wysok

Lipiński

et al. 2016

Journal of Apicultural Science

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A total of 102 honey samples collected from small apiaries (≤ 20 hives) in Poland were analysed for the presence of Clostridium botulinum spores. The samples were prepared using the dilution centrifugation method and cultured in parallel in cooked meat medium (CMM) and tripticase peptone glucose yeast (TPGY) enrichment broths. Identification of toxin types A, B, and E of Clostridium botulinum strains was performed with the use of the multiplex PCR method. Positive samples were also subjected to quantitative analysis with the use of Clostridium botulinum Isolation Agar Base (CBAB). The prevalence analysis showed 22 (21.6%) samples contaminated with C. botulinum spores. The major serotype detected was botulin neurotoxin type A – 16 (72.7%) whereas type B was found in 3 (13.6%) honey samples and type E also only in 3 (13.6%) honey samples. Dual-toxin-producing strains were noted. The average quantity of spores in PCR - C. botulinum positive samples was 190 in 1 gram of honey.

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“…Botulinum neurotoxins (BoNTs) are potent neurotoxins which cause botulism disease. These are produced by various species of clostridia and eight types of BoNTs have been identified (designated with a letter A-H) [1] C. botulinum produces BoNT types A-F and H. C. baratii produces type F, C. butyricum BoNT type E and C. argentinense BoNT type G [1,2]. BoNT types A, B, E, F and H cause disease in humans, types B and D cause botulism in herbivorous mammals (equine and cattle) and type C mainly affects birds [3].…”

Section: Case Reportmentioning

confidence: 99%

Avian Botulism Type C in a Commercial Poultry Farm: First Report in Central America

Rodríguez-Cavallini¹,

López-Ureña²,

Román³

et al. 2018

J Bacteriol Parasitol

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Avian botulism outbreaks are frequently produced by type C neurotoxin secreted by Clostridium botulinum proliferating in decomposing bird carcasses and contaminated soils or water sediments. In this study, a botulism outbreak was diagnosed in broilers from a Costa Rican commercial farm through clinical signs, absence of postmortem histopathological lesions, and the confirmation of toxin in the serum of the birds. C. botulinum was furthered isolated from the intestine of these animals. This is the first report of avian botulism due to C. botulinum type C in Central America.

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Clostridium botulinum in honey: prevalence and antibiotic susceptibility of isolated strains

Cited by 9 publications

References 15 publications

Physicochemical Properties of Imported and Locally Produced Honey did not translate into its Microbial Quality and Antibacterial Activity

Physicochemical Properties of Imported and Locally Produced Honey did not translate into its Microbial Quality and Antibacterial Activity

Clostridium botulinumSpores Found in Honey from Small Apiaries in Poland

Avian Botulism Type C in a Commercial Poultry Farm: First Report in Central America

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