Clostridium acetobutylicum Biofilm: Advances in Understanding the Basis

Zhang, Huifang; Yang, Pengpeng; Wang, Zhenyu; Li, Mengting; Zhang, Jie; Liu, Dong; Chen, Yong; Ying, Hanjie

doi:10.3389/fbioe.2021.658568

Cited by 6 publications

(2 citation statements)

References 52 publications

(85 reference statements)

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“…This transitional period requires that cells undergo multiple physiological changes depending on environmental conditions. Cells need to cease growth, initiate spore formation, and/or produce large-scale macromolecular aggregate structures on which cells can maintain a vegetative stage, including biofilm formation ( 50 ).…”

Section: Resultsmentioning

confidence: 99%

Genetic disruption of the bacterial raiA motif noncoding RNA causes defects in sporulation and aggregation

Soares,

King,

Fernando

et al. 2024

Proc. Natl. Acad. Sci. U.S.A.

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Several structured noncoding RNAs in bacteria are essential contributors to fundamental cellular processes. Thus, discoveries of additional ncRNA classes provide opportunities to uncover and explore biochemical mechanisms relevant to other major and potentially ancient processes. A candidate structured ncRNA named the “ raiA motif” has been found via bioinformatic analyses in over 2,500 bacterial species. The gene coding for the RNA typically resides between the raiA and comFC genes of many species of Bacillota and Actinomycetota. Structural probing of the raiA motif RNA from the Gram-positive anaerobe Clostridium acetobutylicum confirms key features of its sophisticated secondary structure model. Expression analysis of raiA motif RNA reveals that the RNA is constitutively produced but reaches peak abundance during the transition from exponential growth to stationary phase. The raiA motif RNA becomes the fourth most abundant RNA in C. acetobutylicum , excluding ribosomal RNAs and transfer RNAs. Genetic disruption of the raiA motif RNA causes cells to exhibit substantially decreased spore formation and diminished ability to aggregate. Restoration of normal cellular function in this knock-out strain is achieved by expression of a raiA motif gene from a plasmid. These results demonstrate that raiA motif RNAs normally participate in major cell differentiation processes by operating as a trans-acting factor.

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Section: Resultsmentioning

confidence: 99%

Genetic disruption of the bacterial raiA motif noncoding RNA causes defects in sporulation and aggregation

Soares,

King,

Fernando

et al. 2024

Proc. Natl. Acad. Sci. U.S.A.

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show abstract

“…In addition, the reason may be that the reabsorption capacity of organic acids was enhanced and the harmful substances around C. acetobutylicum were reduced with adsorption carriers. In addition, C. acetobutylicum can interact with the adsorption carrier to form a cross-linked structure to promote the formation of the cell membrane, improving the tolerance of the strain [ 18 ]. These results showed that the closed circuit and adsorption carrier had a positive effect on fermentation performance.…”

Section: Resultsmentioning

confidence: 99%

Coproduction and enhancement of electricity and biobutanol using adsorption carrier solid-state fermentation

Feng

Wang

Chen

2022

Biotechnol Biofuels

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Background Electric energy is not collected and utilized in biobutanol fermentation. The reason is that the yields of electron shuttles and nanowires are not enough to gather and transfer all electrons to the electrode in liquid fermentation. However, the solid matrix of the adsorption carrier may be conducive to the collection and transfer of electrons because of its good adsorption and conductivity. Therefore, this first-attempt study coupled microbial fuel cell (MFC) with adsorption carrier solid-state fermentation (ACSF). In addition, the effect and mechanism of adsorption carrier solid-state fermentation on power generation were explored. Results The power generation performance and fermentation performance were improved by ACSF. The power density by polyurethane and carbon felt carrier solid-state fermentation (PC) was 12 times that by no carrier fermentation (NC). The biobutanol yield of absorbent cotton and carbon felt carrier solid-state fermentation (ACC) was increased by 36.86%. Moreover, the mechanism was explored via metabolic flux analysis, cyclic voltammetry and scanning electron microscopy. The results of metabolic flux analysis showed that more electrons were produced and more carbon flowed to biobutanol production. The cyclic voltammetry results revealed that more riboflavin was produced to enhance extracellular electron transport (EET) by ACSF. The scanning electron microscopy image showed that the adsorption capacity and aggregation degree of bacteria were increased on the electrode and nanowires were observed by ACSF. Conclusions A new fermentation mode was established by coupling MFC with ACSF to improve substrate utilization, which will provide crucial insights into the fermentation industry. In addition, the ACSF is an effective method to enhance power generation performance and fermentation performance. Graphical Abstract

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Process Integration and Intensification of Biobutanol Production

Bishai

2023

Production of Biobutanol From Biomass

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Clostridium acetobutylicum Biofilm: Advances in Understanding the Basis

Cited by 6 publications

References 52 publications

Genetic disruption of the bacterial raiA motif noncoding RNA causes defects in sporulation and aggregation

Genetic disruption of the bacterial raiA motif noncoding RNA causes defects in sporulation and aggregation

Coproduction and enhancement of electricity and biobutanol using adsorption carrier solid-state fermentation

Process Integration and Intensification of Biobutanol Production

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