Closing the Loop: Developing an Integrated Design, Make, and Test Platform for Discovery

Parry, David M.

doi:10.1021/acsmedchemlett.9b00095

Cited by 28 publications

(24 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The stability factors in the hanging drop network were the speed of flow and droplet radii. The computer simulation results showed that the taper-tube culture method used a deeper flow tube and had a lower curvature interface that caused higher stability of droplet [10,37]. In the taper-shaped microfluid chip, the suspension unit had a hole with a narrow upper and lower width and the cross-section formed a wedge shape.…”

Section: Discussionmentioning

confidence: 99%

“…As shown in the curve of the water volume fraction as a function of time, the taper-shaped tray was relatively efficient. For liquid exchange, the effectiveness and the efficiency of the medium exchange process were determined by observing the volume fraction changes in moisture in the simulation area at different time points [37]. Combined with the peristaltic pump, the taper-shaped tray was found to be more efficient discharged liquid from the outlet at a constant rate to achieve liquid exchange that can effectively solve the disadvantages of the traditional hanging drop culture method which cannot replace the culture medium.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

A Dynamic Hanging-Drop System for Mesenchymal Stem Cell Culture

Huang

Tzeng

Chen

et al. 2020

IJMS

View full text Add to dashboard Cite

There have been many microfluid technologies combined with hanging-drop for cell culture gotten developed in the past decade. A common problem within these devices is that the cell suspension introduced at the central inlet could cause a number of cells in each microwell to not regularize. Also, the instability of droplets during the spheroid formation remains an unsolved ordeal. In this study, we designed a microfluidic-based hanging-drop culture system with the design of taper-tube that can increase the stability of droplets while enhancing the rate of liquid exchange. A ring is surrounding the taper-tube. The ring can hold the cells to enable us to seed an adequate amount of cells before perfusion. Moreover, during the period of cell culture, the mechanical force around the cell is relatively low to prevent stem cells from differentiate and maintain the phenotype. As a result of our hanging system design, cells are designed to accumulate at the bottom of the droplet. This method enhances convenience for observation activities and analysis of experiments. Thus, this microfluid chip can be used as an in vitro platform representing in vivo physiological conditions, and can be useful in regenerative therapy.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

A Dynamic Hanging-Drop System for Mesenchymal Stem Cell Culture

Huang

Tzeng

Chen

et al. 2020

IJMS

View full text Add to dashboard Cite

show abstract

“…Recent reports show how appealing flow chemical processes are nowadays, in what concerns safety (the need to handle hazardous, toxic or labile chemicals can be avoided), process scale‐up ability (most of the times it is more straightforward than with conventional methods) and profitability (high‐value products can be obtained in a 24/7 manner, due to high levels of automatization, inline quality control technologies and purification techniques). Furthermore, other well‐established techniques can be coupled with continuous‐flow setups, allowing the development of automated drug discovery platforms . Consequently, the future of flow chemistry might be oriented to the ability to increase new molecule output of drug discovery programs, while facilitating and accelerating the identification of hit compounds.…”

Section: Discussionmentioning

confidence: 99%

Flow Chemistry: Towards A More Sustainable Heterocyclic Synthesis

2019

View full text Add to dashboard Cite

Flow Chemistry is a revolutionary field in Organic Chemistry. By changing the conventional methods and apparatus applied in the chemical synthesis, flow chemistry emerges as a game changer for organic synthesis laboratories, in both academia and industries. Considered as a sustainable practice, flow processes play an important role in the development of fine chemical products and in drug discovery development pro- [a] The main advantages of flow chemistry are that it allows the reaction to be carried out safely with high selectivity, high reproducibility and reduced energy consumption/carbon footprint. These features make this emerging technology for organic synthesis desirable for green and sustainable chemical synthesis, giving the chemist time for work planning and design, instead of using it in repetitive tasks, [5b,12] as expressed by the CHEM21 project by awarding a green flag for continuousflow reactions, in opposition to an amber flag for those performed under batch conditions. [13] Pedro Brandão received his MSc in Pharmaceutical Sciences from the Faculty of Pharmacy -University of Porto, in 2011. Pursuing his passion on studies in the field of Drug Discovery and Development, he is currently undergoing his PhD studies in Chemistry, in the field of Catalysis and Sustainability. His main focus of work is the discovery of new drug candidates through sustainable techniques. Marta Pineiro received her Ph.D. in Organic Chemistry in 2003 in the University of Coimbra, Portugal, and since 2002 has been enrolled at the University of Coimbra, being at present Auxiliary Professor. Her research interests are in the area of sustainable organic synthesis, microwave-assisted organic synthesis and synthesis and biological applications of tetrapyrrolic macrocycles. Teresa M. V. D. Pinho e Melo studied Chemistry at the University of Coimbra, where she graduated in 1985, got her M. 7191CPBA) as the oxidizing agent, avoiding the handling of this expensive and explosive reagent. [25] Recently, Morodo et al. explored the synthesis of two very important oxiranes (epichlorohydrin and glycidol) from biobased glycerol, under flow conditions. As depicted in Scheme 1, and using pimelic acid as the catalyst (10 mol-%), a sequential hydrochlorination/dichlorination process allows high conversion of glycerol (> 99 %) into 1,3-dichloro-2-propanol, followed by the quantitative conversion into a separable mixture of glycidol and epichlorohydrin (2:3). The separation of these two products is performed by an in-line membrane separation system, which allows the first product to be collected in the aqueous phase, while the second is collected in MTBE. This heterocycle can be further used as a synthetic precursor to relevant APIs bearing -amino alcohol moieties (e.g., propranolol, alprenolol and naftopidil). [26] Scheme 1. Sequential flow setup for the preparation of glycidol and epichlorohydrin, important APIs synthetic intermediates.Scheme 3. Synthesis and inline purification of a bicyclic aziridine (SGMR = silicon-glass microfluidic react...

show abstract

“…The potential value of automated design-make-test-analyze (DMTA) cycles in drug discovery is substantial 126,183 . Benefits of automation include 131 :…”

Section: Box 5 Reducing Cycle Times By Automationmentioning

confidence: 99%

Rethinking drug design in the artificial intelligence era

Schneider

Walters

Plowright

et al. 2019

Nat Rev Drug Discov

523

349

View full text Add to dashboard Cite

Perspective │2 Artificial intelligence (AI) tools are increasingly being applied in drug discovery. Whilst some protagonists point to vast opportunities potentially offered by such tools, others remain skeptical, waiting for a clear impact to be shown in drug discovery projects. The truth is probably somewhere between these extremes, but it is clear that AI is providing new challenges not only for the scientists involved but also for the biopharma industry and its established processes for discovering and developing new medicines. This article presents the views of a diverse group of international experts on the 'grand challenges' for small-molecule drug discovery with AI and approaches to address them.

show abstract

Closing the Loop: Developing an Integrated Design, Make, and Test Platform for Discovery

Cited by 28 publications

References 21 publications

A Dynamic Hanging-Drop System for Mesenchymal Stem Cell Culture

A Dynamic Hanging-Drop System for Mesenchymal Stem Cell Culture

Flow Chemistry: Towards A More Sustainable Heterocyclic Synthesis

Rethinking drug design in the artificial intelligence era

Contact Info

Product

Resources

About