Closed Genome Sequences of 28 Foodborne Pathogens from the CFSAN Verification Set, Determined by a Combination of Long and Short Reads

González-Escalona, Narjol; Kastanis, George; Timme, Ruth; Roberson, Dwayne; Balkey, Maria; Tallent, Sandra M.

doi:10.1128/mra.00152-20

Cited by 3 publications

(4 citation statements)

References 10 publications

(4 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The pLw showed more than 99% similarity to the plasmids of L. innocua strains isolated from food [55] and to the plasmids of L. monocytogenes strains isolated from food and from clinical sources in different years and different geographical locations [56-59] (Table 4). The L. monocytogenes strains belonged to ST3 of PLI and to different STs of PLII.…”

Section: Virulence Factorsmentioning

confidence: 99%

Genomic characteristics of listeria that caused invasive listeriosis during the COVID-19 pandemic

Voronina

Kunda

Рыжова

et al. 2022

Preprint

View full text Add to dashboard Cite

Invasive listeriosis is relatively rare, but is one of the deadliest food-borne infections, affecting pregnant women, their fetuses and newborn infants, the elderly and immunocompromised people. The aim of this study was to research the impact of the COVID-19 pandemic on invasive listeriosis in the metropolis. Loci and whole-genome sequencing with subsequent bioinformatic analysis were used for the study of clinical and food Listeria monocytogenes isolates revealed in 2018–2022. The results indicate the crucial change in the spectrum of the L. monocytogenes sequence types (ST) causing invasive listeriosis during the COVID-19 pandemic, with slight changes in the ST spectrum of the food isolates. An increase in sensitivity to previously non-human L. monocytogenes genotypes, namely ST8, 20, 21, 37, 391, and 425, was observed. L. monocytogenes of ST20 and 425 carried plasmids with virulence factors (VF), in addition to the 42 VF identified in the genomes with the vip gene exclusion in the genomes of ST7, 8, 21, and 37. Perinatal listeriosis cases were associated with the new hypervirulent L. monocytogenes of ST1, 4, and 219 compiled with old ST6. These data indicate the need for the more stringent control of food products for high-risk groups.

show abstract

Section: Virulence Factorsmentioning

confidence: 99%

Genomic characteristics of listeria that caused invasive listeriosis during the COVID-19 pandemic

Voronina

Kunda

Рыжова

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…The following are available online at https://www.mdpi.com/article/ 10.3390/ijms221910320/s1. References [6,7,12,13,19,22,[24][25][26]28,40,41,57,73,[102][103][104][105][106][107][108][109][110][111][112][113][114] are cited in the supplementary materials…”

Section: Supplementary Materialsmentioning

confidence: 99%

“…The following are available online at . References [ 6 , 7 , 12 , 13 , 19 , 22 , 24 , 25 , 26 , 28 , 40 , 41 , 57 , 73 , 102 , 103 , 104 , 105 , 106 , 107 , 108 , 109 , 110 , 111 , 112 , 113 , 114 ] are cited in the supplementary materials.…”

mentioning

confidence: 99%

Plasmidome of Listeria spp.—The repA-Family Business

Chmielowska

Korsak

Chapkauskaitse

et al. 2021

IJMS

View full text Add to dashboard Cite

Bacteria of the genus Listeria (phylum Firmicutes) include both human and animal pathogens, as well as saprophytic strains. A common component of Listeria spp. genomes are plasmids, i.e., extrachromosomal replicons that contribute to gene flux in bacteria. This study provides an in-depth insight into the structure, diversity and evolution of plasmids occurring in Listeria strains inhabiting various environments under different anthropogenic pressures. Apart from the components of the conserved plasmid backbone (providing replication, stable maintenance and conjugational transfer functions), these replicons contain numerous adaptive genes possibly involved in: (i) resistance to antibiotics, heavy metals, metalloids and sanitizers, and (ii) responses to heat, oxidative, acid and high salinity stressors. Their genomes are also enriched by numerous transposable elements, which have influenced the plasmid architecture. The plasmidome of Listeria is dominated by a group of related replicons encoding the RepA replication initiation protein. Detailed comparative analyses provide valuable data on the level of conservation of these replicons and their role in shaping the structure of the Listeria pangenome, as well as their relationship to plasmids of other genera of Firmicutes, which demonstrates the range and direction of flow of genetic information in this important group of bacteria.

show abstract

“…The reads were trimmed with Trimmomatic v0.36 (9). The final complete genome sequence (comprising the chromosome and plasmid, when present) for each strain was obtained using a previously described pipeline (10), except that Flye v2.6 (11) was used instead of Canu v1.7 (12) for long-read de novo assembling. The genomes were confirmed as circular closed by finding the contig end overlap, which was then manually trimmed.…”

mentioning

confidence: 99%

Complete Genome Sequences of 17 Clinical Campylobacter jejuni Strains from Chile

Bravo

Porte

Weitzel

et al. 2020

Microbiol Resour Announc

Self Cite

View full text Add to dashboard Cite

show abstract

Closed Genome Sequences of 28 Foodborne Pathogens from the CFSAN Verification Set, Determined by a Combination of Long and Short Reads

Cited by 3 publications

References 10 publications

Genomic characteristics of listeria that caused invasive listeriosis during the COVID-19 pandemic

Genomic characteristics of listeria that caused invasive listeriosis during the COVID-19 pandemic

Plasmidome of Listeria spp.—The repA-Family Business

Complete Genome Sequences of 17 Clinical Campylobacter jejuni Strains from Chile

Contact Info

Product

Resources

About