Closed Genome Sequence of an Environmental Aeromonas veronii Strain from California, United States, with an IncA/C Plasmid Carrying an Extended-Spectrum β-Lactamase Gene,
            <i>bla</i>
            <sub>VEB-3</sub>

Lovero, Karissa G.; Mota-Bravo, Luis

doi:10.1128/mra.01033-21

Cited by 3 publications

(2 citation statements)

References 14 publications

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“…The genomes of 19 other A. veronii strains were obtained from the NCBI Genome database using the NCBI Datasets command-line tool with the parameters ‘datasets download genome taxon “aeromonas veronii” --assembly-level complete_genome --assembly-source refseq --dehydrated’ in November 2021. RefSeq assembly accession numbers are provided as follows: GCF_000204115.1 [ 43 ], GCF_000464515.2 [ 44 ], GCF_001593245.1, GCF_002803925.1, GCF_002803945.1, GCF_003491365.1, GCF_003722175.1, GCF_008693705.1 [ 45 ], GCF_009755745.1, GCF_009834065.1, GCF_011045495.1, GCF_013415825.1, GCF_014168715.1 [ 46 ], GCF_014168995.1 [ 46 ], GCF_014169795.1 [ 46 ], GCF_014169835.1 [ 46 ], GCF_014169875.1 [ 46 ], GCF_017310275.1 and GCF_020172765.2 [ 47 ]. To ensure the timeliness and comprehensiveness of the data, we also removed the restriction of assembly level and downloaded 232 A .…”

Section: Methodsmentioning

confidence: 99%

Acquisition of Type I methyltransferase via horizontal gene transfer increases the drug resistance of Aeromonas veronii

Ma,

Zhao,

et al. 2023

Microbial Genomics

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Aeromonas veronii is an opportunistic pathogen that affects both fish and mammals, including humans, leading to bacteraemia, sepsis, meningitis and even death. The increasing virulence and drug resistance of A. veronii are of significant concern and pose a severe risk to public safety. The Type I restriction-modification (RM) system, which functions as a bacterial defence mechanism, can influence gene expression through DNA methylation. However, little research has been conducted to explore its origin, evolutionary path, and relationship to virulence and drug resistance in A. veronii . In this study, we analysed the pan-genome of 233 A . veronii strains, and the results indicated that it was 'open', meaning that A. veronii has acquired additional genes from other species. This suggested that A. veronii had the potential to adapt and evolve rapidly, which might have contributed to its drug resistance. One Type I methyltransferase (MTase) and two complete Type I RM systems were identified, namely AveC4I, AveC4II and AveC4III in A. veronii strain C4, respectively. Notably, AveC4I was exclusive to A. veronii C4. Phylogenetic analysis revealed that AveC4I was derived from horizontal gene transfer from Thiocystis violascens and exchanged genes with the human pathogen Comamonas kerstersii . Single molecule real-time sequencing was applied to identify the motif methylated by AveC4I, which was unique and not recognized by any reported MTases in the REBASE database. We also annotated the functions and pathways of the genes containing the motif, revealing that AveC4I may control drug resistance in A. veronii C4. Our findings provide new insight on the mechanisms underlying drug resistance in pathogenic bacteria. By identifying the specific genes and pathways affected by AveC4I, this study may aid in the development of new therapeutic approaches to combat A. veronii infections.

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Section: Methodsmentioning

confidence: 99%

Acquisition of Type I methyltransferase via horizontal gene transfer increases the drug resistance of Aeromonas veronii

Ma,

Zhao,

et al. 2023

Microbial Genomics

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“…All except 4 C3 isolates carried an IncA/C plasmid; 17 had the IncA/C_1_FJ705807 replicon type, and 5 had the IncA/C2_1_JN157804 replicon type. We further determined that the IncA/C_1_FJ705807 type plasmid is a novel plasmid that was most closely related to Aeromonas veronii plasmid p158496 (26), whereas the IncA/C2_1_JN157804 type plasmid was most similar to V. cholerae O139 pVC211 (GenBank accession no. KY399978.1).…”

Section: Association Of Plasmids and Integrative And Conjugative Elem...mentioning

confidence: 99%

Genomic Epidemiology of Vibrio cholerae O139, Zhejiang Province, China, 1994–2018

Luo¹,

Ye²,

Payne³

et al. 2022

Emerg. Infect. Dis.

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Cholera caused by Vibrio cholerae O139 was first reported in Bangladesh and India in 1992. To determine the genomic epidemiology and origins of O139 in China, we sequenced 104 O139 isolates collected from Zhejiang Province, China, during 1994-2018 and compared them with 57 O139 genomes from other countries in Asia. Most Zhejiang isolates fell into 3 clusters (C1-C3), which probably originated in India (C1) and Thailand (C2 and C3) during the early 1990s. Different clusters harbored different antimicrobial resistance genes and IncA/C plasmids. The integrative and conjugative elements carried by Zhejiang isolates were of a new type, differing from ICEV-chInd4 and SXT MO10 by single-nucleotide polymorphisms and presence of genes. Quinolone resistance-conferring mutations S85L in parC and S83I in gyrA occurred in 71.2% of the Zhejiang isolates. The ctxB copy number differed among the 3 clusters. Our findings provided new insights for prevention and control of O139 cholera .

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Dissemination of IncC plasmids in Salmonella enterica serovar Thompson recovered from seafood and human diarrheic patients in China

Zhang,

Kuang,

et al. 2024

International Journal of Food Microbiology

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Closed Genome Sequence of an Environmental Aeromonas veronii Strain from California, United States, with an IncA/C Plasmid Carrying an Extended-Spectrum β-Lactamase Gene, bla _VEB-3

Abstract: We describe the extended-spectrum β-lactamase bla VEB-3 gene found in an IncA/C plasmid in Aeromonas veronii strain SW3814, which was collected from a freshwater lake in southern California, United States.

Cited by 3 publications

References 14 publications

Acquisition of Type I methyltransferase via horizontal gene transfer increases the drug resistance of Aeromonas veronii

Acquisition of Type I methyltransferase via horizontal gene transfer increases the drug resistance of Aeromonas veronii

Genomic Epidemiology of Vibrio cholerae O139, Zhejiang Province, China, 1994–2018

Dissemination of IncC plasmids in Salmonella enterica serovar Thompson recovered from seafood and human diarrheic patients in China

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Closed Genome Sequence of an Environmental Aeromonas veronii Strain from California, United States, with an IncA/C Plasmid Carrying an Extended-Spectrum β-Lactamase Gene, bla VEB-3

Abstract: We describe the extended-spectrum β-lactamase bla VEB-3 gene found in an IncA/C plasmid in Aeromonas veronii strain SW3814, which was collected from a freshwater lake in southern California, United States.

Cited by 3 publications

References 14 publications

Acquisition of Type I methyltransferase via horizontal gene transfer increases the drug resistance of Aeromonas veronii

Acquisition of Type I methyltransferase via horizontal gene transfer increases the drug resistance of Aeromonas veronii

Genomic Epidemiology of Vibrio cholerae O139, Zhejiang Province, China, 1994–2018

Dissemination of IncC plasmids in Salmonella enterica serovar Thompson recovered from seafood and human diarrheic patients in China

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Closed Genome Sequence of an Environmental Aeromonas veronii Strain from California, United States, with an IncA/C Plasmid Carrying an Extended-Spectrum β-Lactamase Gene, bla _VEB-3