Closed Genome Sequence of a Salmonella enterica Serotype Senftenberg Strain Carrying the
            <i>mcr-9</i>
            Gene Isolated from Broken Chicken Eggshells in Trinidad and Tobago

Maguire, Meghan; Khan, Anisa S.; Adesiyun, Abiodun A.; Georges, Karla; González-Escalona, Narjol

doi:10.1128/mra.01465-20

Cited by 8 publications

(6 citation statements)

References 11 publications

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“…We could not find a single study on antimicrobial resistance genes in Salmonella from pigs in the Caribbean, and only few on Salmonella as a whole in the Caribbean. In poultry strains in Trinidad, a quite higher prevalence of resistance was found compared to our study, and this included plasmid-mediated colistin resistance [36][37][38].…”

Section: Discussioncontrasting

confidence: 64%

Salmonella in Pig Farms and on Pig Meat in Suriname

2021

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Salmonella is one of the most important food borne zoonotic pathogens. While mainly associated with poultry, it has also been associated with pigs. Compared to the high-income countries, there is much less known on the prevalence of Salmonella in low- and middle-income countries, especially in the Caribbean area. Therefore, we investigated the prevalence of Salmonella in pigs and pig meat in Suriname. A total of 53 farms and 53 meat samples were included, and Salmonella was isolated using standard protocols. Strains were subjected to whole genome sequencing. No Salmonella was found on pig meat. Five farms were found to be positive for Salmonella, and a total of eight different strains were obtained. Serotypes were S. Anatum (n = 1), S. Ohio (n = 2), a monophasic variant of S. Typhimurium (n = 3), one S. Brandenburg, and one S. Javaniana. The monophasic variant of S. Typhimurium belonged to the ST34 pandemic clone, and the three strains were very similar. A few resistance genes, located on mobile genetic elements, were found. Several plasmids were detected, though only one was carrying resistance genes. This is the first study on the prevalence of Salmonella in pigs in the Caribbean and that used whole genome sequencing for characterization. The strains were rather susceptible. Local comparison of similar serotypes showed a mainly clonal spread of certain serotypes.

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Section: Discussioncontrasting

confidence: 64%

Salmonella in Pig Farms and on Pig Meat in Suriname

2021

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“…Enteritidis (Li et al, 2021 ). We have additionally detected the presence of the mcr-9 gene in another strain isolated from this overall study (Maguire et al, 2021 ). The mcr-9 gene variant was described in 2019 (Carroll et al, 2019 ) and appears not to confer colistin resistance in Salmonella and Escherichia coli (Tyson et al, 2020 ).…”

Section: Resultssupporting

confidence: 51%

Genomic Comparison of Eight Closed Genomes of Multidrug-Resistant Salmonella enterica Strains Isolated From Broiler Farms and Processing Plants in Trinidad and Tobago

et al. 2022

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Salmonella enterica is an important foodborne pathogen worldwide. We used long and short-read sequencing to close genomes of eight multidrug-resistant (MDR) S. enterica strains, belonging to serovars Infantis (2), Albany, Oranienburg, I 4,[5],12:i:-, Javiana, Schwarzengrund, and Kentucky from broiler chicken farms and processing plants in Trinidad and Tobago. They also belonged to seven different sequence types (STs- 32, 292, 1510, 19, 24, 152, and 96). Among the strains, seven had demonstrated multi-drug resistance with the presence of at least three AMR genes, whereas three isolates contained the quinolone resistance gene qnrB19 in plasmids (CFSAN103840, CFSAN103854, and CFSAN103872). The extended-spectrum β-lactamase genes blaCTX−M−65 (CFSAN103796) and blaTEM−1 (CFSAN103852) were detected in this study. The genomes closed in this study will be useful for future source tracking and outbreak investigations in Trinidad and Tobago and worldwide.

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“…Arkali et al [ 68 ] detected the sul1 gene among 58% of Salmonella isolated from chickens in Eastern Turkey. The detection of one mobilized colistin resistance mcr-9.1 gene [ 69 ] in an isolate of serovar Senftenberg was not a significant finding. This gene is not associated with colistin resistance in Salmonella or E. coli in the United States [ 70 ].…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization of Salmonella Detected along the Broiler Production Chain in Trinidad and Tobago

et al. 2022

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This cross-sectional study determined the serovars, antimicrobial resistance genes, and virulence factors of Salmonella isolated from hatcheries, broiler farms, processing plants, and retail outlets in Trinidad and Tobago. Salmonella in silico serotyping detected 23 different serovars where Kentucky 20.5% (30/146), Javiana 19.2% (28/146), Infantis 13.7% (20/146), and Albany 8.9% (13/146) were the predominant serovars. There was a 76.0% (111/146) agreement between serotyping results using traditional conventional methods and whole-genome sequencing (WGS) in in silico analysis. In silico identification of antimicrobial resistance genes conferring resistance to aminoglycosides, cephalosporins, peptides, sulfonamides, and antiseptics were detected. Multidrug resistance (MDR) was detected in 6.8% (10/146) of the isolates of which 100% originated from broiler farms. Overall, virulence factors associated with secretion systems and fimbrial adherence determinants accounted for 69.3% (3091/4463), and 29.2% (1302/4463) counts, respectively. Ten of 20 isolates of serovar Infantis (50.0%) showed MDR and contained the blaCTX-M-65 gene. This is the first molecular characterization of Salmonella isolates detected along the entire broiler production continuum in the Caribbean region using WGS. The availability of these genomes will help future source tracking during epidemiological investigations associated with Salmonella foodborne outbreaks in the region and worldwide.

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Closed Genome Sequence of a Salmonella enterica Serotype Senftenberg Strain Carrying the mcr-9 Gene Isolated from Broken Chicken Eggshells in Trinidad and Tobago

Cited by 8 publications

References 11 publications

Salmonella in Pig Farms and on Pig Meat in Suriname

Salmonella in Pig Farms and on Pig Meat in Suriname

Genomic Comparison of Eight Closed Genomes of Multidrug-Resistant Salmonella enterica Strains Isolated From Broiler Farms and Processing Plants in Trinidad and Tobago

Molecular Characterization of Salmonella Detected along the Broiler Production Chain in Trinidad and Tobago

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