Closed Continuous-flow Centrifuge

Judson, George; Jones, Andrew; Kellogg, R. M.; Buckner, Dean; Eisel, Robert; Perry, Seymour; Greenough, William B.

doi:10.1038/217816a0

Cited by 73 publications

(18 citation statements)

References 1 publication

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“…NCI‐IBM blood cell separator was developed in the 1960s to provide an effective, reliable, closed centrifuge system in which a mixture of blood and anticoagulant could undergo uninterrupted processing while collection of components was in progress (7,8).…”

Section: Resultsmentioning

confidence: 99%

Principles of Blood Separation and Component Extraction in a Disposable Continuous‐Flow Single‐Stage Channel

Hester

Kellogg

Mulzet

et al. 2000

Therapeutic Apheresis

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Guest Editor's Introduction: By 1977, the NCI/IBM 2990 and the Amico Celltrifuge, a conceptually similar instrument that had an identical centrifuge bowl, were being used worldwide. However, a major technical problem remained in that the blood path was still not completely disposable. To overcome this drawback, IBM 2977 was developed. The centrifuge bowl was changed from an axial flow separator to a circumferential flow geometry. The IBM 2997 single‐stage channel instrument was first introduced and followed by the dual‐stage channel instrument to obtain platelet concentrate with minimum lymphocyte contamination. This paper describes the basic design, operation conditions, and collection performance of the single‐stage channel IBM 2977. This paper was printed in Blood, vol 54, page 254–268 (1979) and reprinted here with permission. A single‐stage disposable channel and seal that provides for leukocyte and granulocyte collection by continuous‐flow cell separation (CFCS) has been designed by the IBM Corporation. This paper describes (1) the separation characteristics of whole blood as it responds to varying gravitational (G) forces and flow rates through the channel; (2) the mechanism by which the buffy coat accumulates and is extracted; (3) the efficiency of extraction; (4) those donor and procedural variables that contribute to the final yield; (5) posttransfusion increment response in patients; and (6) the functional integrity of the cells collected.

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Section: Resultsmentioning

confidence: 99%

Principles of Blood Separation and Component Extraction in a Disposable Continuous‐Flow Single‐Stage Channel

Hester

Kellogg

Mulzet

et al. 2000

Therapeutic Apheresis

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“…The use of the continuous flow ceIItrifuge for plasma dialysis has been suggested as a means of applying extremes of pH, osmotic gradients and hydrostatic pressure which would not be withstood by erythrocytes (Judson, Jones, Kellog, Buckner, Eisel, Perry & Greenough, 1968). Because whole blood perfusion of resins is sometimes accompanied by pressure problems and haemolysis, the celltrifuge also offers a means of perfusing resins with plasma, thus avoiding the complications due to the presence of erythrocytes.…”

Section: Discussionmentioning

confidence: 99%

Resin Column Perfusion with Whole Blood or Plasma Separated by the Continuous Flow Celltrifuge

et al. 1975

Clinical Science

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1. The aim of this study was to define the factors influencing plasma separation from the continuous flow celltrifuge and to evaluate plasma as an alternative to whole blood for perfusion of exchange resins as part of a system of artificial liver support. 2. Studies in vitro showed the importance of packed cell volume, centrifugal force and duration of centrifugation on the degree of plasma separation. From these data it was possible to calculate plasma flow rates likely to be obtained from the celltrifuge when used in vivo. These predicted values correlated closely with plasma flow rate obtained in twenty-six studies in dogs. 3. Comparison of whole blood perfusion with plasma perfusion of exchange resins in another series of dog experiments showed that with whole blood perfusion there was often a considerable rise in pressure across the resin column but that this did not occur with plasma perfusion. 4. Measurements of platelet losses in the same series of experiments showed a 50% reduction of arterial platelet counts over a 31/2 h period of perfusion when whole blood was perfused. Although the fall was lower with plasma perfusion, the difference was not statistically significant. 5. Use of the celltrifuge provides a means of resin perfusion free of the mechanical difficulties of whole blood perfusion, but platelet losses still remain a problem.

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“…The NCI-IBM continuous flow blood cell centrifuge was adapted for use in the dog, based in part on the recent findings of Judson, Jones, Buckner, and associates (Judson et al, 1968;Jones, 1968;Buckner et al, 1968).…”

Section: Methodsmentioning

confidence: 99%

The effect of lymphocyte depletion by continuous flow centrifugation in canine renal allotransplants

Murphy

Williams

Brede

et al. 1970

Journal of Surgical Oncology

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The NCI-IBM continuous flow separator was adapted for use in untreated dog renal allotransplants. Studies in 39 dogs demonstrated that a 2-hr period a t 1000 rpm with regional heparinization and temporary femoral vessel cannulation would be tolerated.Follow-up hematological studies demonstrated that during the 2-hr treatment an average of 19.4 x lo9 white cells were collected. These contained an average of 7.4 x 109 lymphocytes. Red cells and platelet levels were not markedly affected in treated dogs.This leukopheresis treatment was then employed once in 14 untreated dog renal allotransplants either pretransplant, or a t days 1-4 or 5-7 post-transplant. Eight other renal allotransplants were splenectomized 2 weeks previously and similarly leukopheresed at one of the three treatment periods. Twelve untreated dog renal allografts survived 9.2 f 0.2 days (+ 1 SE). Pretransplant leukopheresis dogs survived 7.8 5 1.5 days. Dogs leukopheresed during 5-7 days post-transplant survived 16.0 -j= 2.7 days (p < 0.01). All splenectomized dogs leukopheresed pre-or post-transplant had survival rates less than 12.8 i 3.1 days. Fifteen azathioprine-treated (5 mg/kg/IM) dog renal transplants lived 14.6 i-0.67 days. Mechanical leukopheresis thus prolongs renal allograft survival, probably by suppressing lymphocyte levels. The effect is limited. Humoral factors are not affected. The leukopheresis effect is most beneficial a t the fifth to seventh day after renal transplant, and it is not improved by prior splenectomy.

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Closed Continuous-flow Centrifuge

Cited by 73 publications

References 1 publication

Principles of Blood Separation and Component Extraction in a Disposable Continuous‐Flow Single‐Stage Channel

Principles of Blood Separation and Component Extraction in a Disposable Continuous‐Flow Single‐Stage Channel

Resin Column Perfusion with Whole Blood or Plasma Separated by the Continuous Flow Celltrifuge

The effect of lymphocyte depletion by continuous flow centrifugation in canine renal allotransplants

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