Close Physical Linkage of Human Troponin Genes: Organization, Sequence, and Expression of the Locus Encoding Cardiac Troponin I and Slow Skeletal Troponin T

Barton, Paul J.R.; Cullen, Martin E.; Townsend, P. J.; Brand, Nigel J.; Mullen, Antony J.; Norman, David A.M.; Bhavsar, Pankaj; Yacoub, Magdi H.

doi:10.1006/geno.1998.5702

Cited by 30 publications

(22 citation statements)

References 44 publications

(54 reference statements)

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“…As has been previously reported (Campinho et al, 2005) and in contrast to what occurs in tetrapods, sea bream sTnT genes have a coordinated expression pattern during sea bream development (Barton et al, 1999;Briggs and Schachat, 1993;Bucher et al, 1999;Farza et al, 1998;Gahlmann et al, 1987;Huang et al, 1999;Jin et al, 1998a;Jin et al, 2000;Perry, 1998;Samson et al, 1994;Wang and Jin, 1997;Wang et al, 2002;Yonemura et al, 2000;Yonemura et al, 2002). Gene expression of sTnT1sb (Fig.·7) and its splice variants in sea bream, but not sTnT2sb and iTnT (Fig.·6), are affected by T3 treatment.…”

Section: Sea Bream Tnt Genes Th Responsivenessmentioning

confidence: 87%

Regulation of troponin T expression during muscle development in sea breamSparus auratusLinnaeus: the potential role of thyroid hormones

Campinho

Sweeney

Power

2006

Journal of Experimental Biology

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SUMMARY In the sea bream Sparus auratus three stage-specific fast troponin T (fTnT) isoforms have been cloned and correspond to embryonic-, larval- and adult-specific isoforms. Characterisation, using database searches, of the putative genomic organisation of Fugu rubripes and Tetraodon nigroviridis fTnT indicates that alternative exon splicing in the 5 region of the gene generates the different isoforms. Moreover, comparison of teleost fTnTs suggests that alternative splicing of fTnT appears to be common in teleosts. A different temporal expression pattern for each fTnT splice varotnt is found during sea bream development and probably relates to differing functional demands, as a highly acidic embryonic form (pI 5.16) is substituted by a basic larval form (pI 9.57). Thyroid hormones (THs), which play an important regulatory role in muscle development in flatfish and tetrapods,appear also to influence TnT gene expression in the sea bream. However, THs have a divergent action on different sea bream TnT genes and although the slow isoform (sTnT1) is TH-responsive, fTnT, sTnT2 and the itronless isoform (iTnT) are unaffected. The present results taken together with those published for flatfish seem to suggest differences may exist in the regulation of larval muscle development in teleosts.

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Section: Sea Bream Tnt Genes Th Responsivenessmentioning

confidence: 87%

Regulation of troponin T expression during muscle development in sea breamSparus auratusLinnaeus: the potential role of thyroid hormones

Campinho

Sweeney

Power

2006

Journal of Experimental Biology

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“…2 A) and 23-kb (HindIII, Fig. 2B) fragments (27,30). After an EcoRI and a HindIII digestion, the M19 and HA16C5 cells showed additional bands, indicating a possible disruption of the TNNT1 gene ( Fig.…”

Section: Tnnt1 Rearrangements In Latently Infectedmentioning

confidence: 95%

“…The positions of the HindIII sites are deduced from published sequences (for TNNT1 and AAVS1) and from sequence data generated for this study (for AAVS1). H*: this HindIII site was deduced from the published cardiac troponin I gene (TNNI3) sequences (30,34). The HindIII site within TNNI3 was included based on the recent observation by Barton et al (30) that the TNNT1 gene is closely linked to the TNNI3 gene.…”

Section: Tnnt1 Rearrangements In Latently Infectedmentioning

confidence: 99%

Adeno-associated virus site-specifically integrates into a muscle-specific DNA region

Dutheil

Shi

Dupressoir

et al. 2000

Proc. Natl. Acad. Sci. U.S.A.

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The nonpathogenic human virus adeno-associated virus type 2 (AAV) has evolved the potentially unique strategy to establish latency by site-specifically integrating its genome into human chromosome 19 (19q13.3-qter) at a locus designated AAVS1. This nonhomologous, site-specific recombination of viral DNA with the human genome provides a basis for developing targeted gene therapy vectors. To assess whether the region surrounding AAVS1 might have contributed to the selection of the specific integration site, we have investigated this locus. Here, we show that AAVS1 is closely linked to the slow skeletal troponin T gene, TNNT1, which has been mapped previously to 19q13.4. In support of this idea, we demonstrate that site-specific AAV DNA integration can result in the formation of TNNT1-AAV junctions. The question now arises whether muscle represents a natural target tissue for latent AAV infection. This possibility is of additional interest in view of recent observations that muscle tissue is particularly well suited for AAV-mediated gene transfer. The question also occurs whether latent infection by AAV can lead to phenotypic changes of the multinucleated muscle fiber cells.T he human parvovirus adeno-associated virus type 2 (AAV) has adopted the strategy of alternate life styles to ensure a successful relationship with its host (1). This nonpathogenic virus productively replicates only in the presence of helper virus co-or superinfection (2). In the absence of such helper conditions, AAV has evolved a strategy potentially unique among eukaryotic viruses. On infection of the non-coinfected host cell, AAV establishes latency by integrating its genome sitespecifically into a region on the q arm of human chromosome 19 (19q13.3-qter) (3, 4), designated AAVS1.The 4.7-kb linear single-stranded genome contains two ORFs and is flanked by inverted terminal repeats (ITRs) (5). The right ORF encodes three capsid proteins, and the left ORF encodes the four nonstructural proteins (Rep proteins) that are involved in regulating all aspects of the viral life style (2).The 145-nt ITRs are the only viral sequences required in cis for DNA replication, packaging of the viral genome into the capsid, and site-specific DNA integration. Within the ITRs, a Rep binding site (RBS) allows for specific recruitment of the large Rep proteins (i.e., Rep68 and Rep78) to the origin of replication (6-8). A Rep-specific endonuclease site (terminal resolution site, TRS) is separated from the RBS by a 13-nt spacer (9-11). Together, RBS and TRS can act as a minimal origin for Rep-mediated DNA replication (12).To dissect the requirements for site specificity in targeted AAV DNA integration, an 8.2-kb fragment of AAVS1 has been isolated from embryonic lung fibroblast (WI38) DNA, and a 4-kb sequence was determined (13). It was shown by using an episomal model system that site specificity is determined by cellular sequences (14) and that a 33-nt sequence is necessary and sufficient for this targeted nonhomologous recombination event to occur (15). This 33-n...

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“…Variants of the chicken sTnT cDNA that differ by the presence or absence of an additional alanine-encoding codon and by single base substitutions at two positions in the 5V-region and four positions in the 3V-region have been found (Yonemura et al, 2000). Although genomic structures of the mouse (Huang et al, 1999) and human (Barton et al, 1999) sTnT genes have been reported, the chicken sTnT gene has not yet been studied. Structural and regulatory analyses of the chicken sTnT gene will permit a direct comparison of genomic organization and transcriptional regulation of the three TnT genes from a single species.…”

Section: Introductionmentioning

confidence: 96%

Genomic structure of the chicken slow skeletal muscle troponin T gene

Hirao

Yonemura²,

Miyazaki

2004

Gene

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Close Physical Linkage of Human Troponin Genes: Organization, Sequence, and Expression of the Locus Encoding Cardiac Troponin I and Slow Skeletal Troponin T

Cited by 30 publications

References 44 publications

Regulation of troponin T expression during muscle development in sea breamSparus auratusLinnaeus: the potential role of thyroid hormones

Regulation of troponin T expression during muscle development in sea breamSparus auratusLinnaeus: the potential role of thyroid hormones

Adeno-associated virus site-specifically integrates into a muscle-specific DNA region

Genomic structure of the chicken slow skeletal muscle troponin T gene

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