Close genetic relationship between Salmonella enterica serovar Enteritidis isolated from patients with diarrhoea and poultry in the Republic of Korea

Lee, Dong‐Hun; Hyeon, Ji-Yeon; Kim, J.; Kim, S.J.; Jeon, Semi; Choi, Soo-Won; Hong, Woo-Tack; Song, Chang‐Seon; Lee, S.-W.

doi:10.1016/j.cmi.2015.05.036

Cited by 7 publications

(5 citation statements)

References 4 publications

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“…To the best of our knowledge, this is the first study that uses WGS to determine the genetic relationship among Salmonella Enteritidis strains isolated from humans and poultry sources in Korea. Previous findings obtained by PFGE and MLVA analysis of Salmonella Enteritidis strains isolated from humans and poultry sources suggest that Salmonella Enteritidis strains have already been transmitted from poultry sources to humans in Korea [14,15]. In this study, results of wgSNP analysis between Salmonella Enteritidis strains isolated from humans and poultry sources were consistent with those of the previous PFGE and MLVA studies.…”

Section: Discussionsupporting

confidence: 91%

“…Epidemiological sources of Salmonella outbreaks have been investigated using pulsedfield gel electrophoresis (PFGE) and multiple-locus variable-number tandem repeats (MLVA) analysis [13]. In previous studies, these approaches have successfully detected the genetic relationship between Salmonella Enteritidis strains isolated from human patients or poultry sources in Korea [14,15]. However, the discrimination power of these methods for genetically closely related Salmonella Enteritidis strains is limited [16,17].…”

Section: Introductionmentioning

confidence: 99%

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Whole-Genome Analysis of Multidrug-Resistant Salmonella Enteritidis Strains Isolated from Poultry Sources in Korea

Kim

Lee

et al. 2021

Pathogens

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The Salmonella Enterica subsp. Enterica serovar Enteritidis is one of main serovars isolated from human patients with food poisoning and poultry without clinical signs. Consumption of poultry products contaminated with Salmonella Enteritidis is a common source of human salmonellosis; 82 Salmonella spp. were isolated from 291 samples of retail chicken meat, 201 one-day-old chicks, 30 internal organs of chickens, 156 chicken eggs, 100 duck eggs, 38 straw bedding samples, 18 samples of retail duck meat, and 19 swab samples from slaughterhouses in 2019 and 2020. An antibiotic susceptibility test was performed for all isolates, revealing 33 multidrug-resistant (MDR) strains. The whole genome of 33 MDR strains isolated in 2019 and 2020 and 10 strains isolated in 2011, 2012, and 2017 was sequenced using the MinION sequencing protocol. Within these 43 samples, 5 serovars were identified: S. Enteritidis, S. Agona, S. Virchow, S. Albany, and S. Bareilly. The most common serovar was S. Enteritidis (26/43), which showed the highest resistance to ampicillin (100%), followed by nalidixic acid (90%) and colistin (83%). Core genome multilocus sequence typing analysis showed that the S. Enteritidis strains isolated from different sources and in different years were clustered together. In addition, the S. Enteritidis strains isolated since 2011 consistently harbored the same antibiotic resistance patterns.

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Whole-Genome Analysis of Multidrug-Resistant Salmonella Enteritidis Strains Isolated from Poultry Sources in Korea

Kim

Lee

et al. 2021

Pathogens

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“…This is the first documented WGS study conducted in the poultry (broilers and layers) industry along the broiler production chain in Trinidad and Tobago, and the Caribbean region at large. Whole-genome sequencing analysis has been used to investigate genetic characteristics and phylogenies among Salmonella strains isolated from different origins, such as humans, food, animals, and the environment [ 33 , 34 , 35 , 36 ]. The current study was comprised of isolates from four cross-sectional studies conducted at the level of retail outlets (2016–2017) [ 25 ], broiler processing plants (2018) [ 23 ], broiler farms, and broiler hatcheries (2019) [ 22 ].…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization of Salmonella Detected along the Broiler Production Chain in Trinidad and Tobago

et al. 2022

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This cross-sectional study determined the serovars, antimicrobial resistance genes, and virulence factors of Salmonella isolated from hatcheries, broiler farms, processing plants, and retail outlets in Trinidad and Tobago. Salmonella in silico serotyping detected 23 different serovars where Kentucky 20.5% (30/146), Javiana 19.2% (28/146), Infantis 13.7% (20/146), and Albany 8.9% (13/146) were the predominant serovars. There was a 76.0% (111/146) agreement between serotyping results using traditional conventional methods and whole-genome sequencing (WGS) in in silico analysis. In silico identification of antimicrobial resistance genes conferring resistance to aminoglycosides, cephalosporins, peptides, sulfonamides, and antiseptics were detected. Multidrug resistance (MDR) was detected in 6.8% (10/146) of the isolates of which 100% originated from broiler farms. Overall, virulence factors associated with secretion systems and fimbrial adherence determinants accounted for 69.3% (3091/4463), and 29.2% (1302/4463) counts, respectively. Ten of 20 isolates of serovar Infantis (50.0%) showed MDR and contained the blaCTX-M-65 gene. This is the first molecular characterization of Salmonella isolates detected along the entire broiler production continuum in the Caribbean region using WGS. The availability of these genomes will help future source tracking during epidemiological investigations associated with Salmonella foodborne outbreaks in the region and worldwide.

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“…Salmonella enterica serovar Enteritidis is the most common cause of human Salmonella infection cases in Korea and many other countries such as the European Union (Hald, 2008;Lee et al, 2015;Wagenaar et al, 2013). The main source of this serotype is known to be contaminated poultry meat and eggs (Foley et al, 2008;Jackson et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Sequential Transmission of Salmonella in the Slaughtering Process of Chicken in Korea

Lee

Youn

Jeong

et al. 2019

Journal of Food Science

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Salmonella is one of the most common foodborne pathogens worldwide. Salmonella infections in humans are mainly associated with consumption of poultry products contaminated with this foodborne pathogen. Therefore, strict sanitary measures are necessary to control Salmonella contamination during the slaughtering process of poultry. The objective of this study was to determine the occurrence and transmission of Salmonella at a series of steps in the slaughtering process of chicken. A total of 601 samples were collected from a series of slaughtering steps (10 sampling sites) of 26 chicken slaughterhouses throughout Korea. Salmonella was isolated from samples and its distribution was analyzed along the slaughtering process. Isolates from each sampling site were tested for susceptibility to 15 antibiotics by the broth microdilution method. They were also genotypically characterized by pulsed‐field gel electrophoresis (PFGE). Salmonela was isolated from 168 out of 601 samples. Sixteen serotypes were identified while six isolates were untypable. Salmonella enterica serovars Montevideo (n = 29) and Virchow (n = 27) were the most common serotypes out of 119 nonredundant isolates. Relatively high contamination rates of Salmonella were found in shackles (75.0%), feathers near plucking machine (68.5%), and feces from crates (44.0%). Twenty‐three antibiotic resistance patterns were recognized and 40 (33.6%) isolates were resistant to five or more antibiotics. The same serotypes of Salmonella were distributed along the slaughtering process of each Salmonella‐positive slaughterhouse. Most of those isolates belonging to the same serotype had identical or closely related PFGE profiles. They also shared common antibiotic resistance patterns. Overall findings of this study indicated that Salmonella were sequentially transmitted through the chicken slaughtering process. Practical Application This study provides useful information on the distribution and transmission of Salmonella serotypes through the chicken slaughtering process. Overall findings indicated the need for routine microbiological monitoring along the slaughtering process. This study also showed that on‐farm control of Salmonella is needed to obtain Salmonella‐free chicken carcasses.

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Close genetic relationship between Salmonella enterica serovar Enteritidis isolated from patients with diarrhoea and poultry in the Republic of Korea

Cited by 7 publications

References 4 publications

Whole-Genome Analysis of Multidrug-Resistant Salmonella Enteritidis Strains Isolated from Poultry Sources in Korea

Whole-Genome Analysis of Multidrug-Resistant Salmonella Enteritidis Strains Isolated from Poultry Sources in Korea

Molecular Characterization of Salmonella Detected along the Broiler Production Chain in Trinidad and Tobago

Sequential Transmission of Salmonella in the Slaughtering Process of Chicken in Korea

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