Cloning Vectors Derived from Animal Viruses

Rigby, Peter

doi:10.1099/0022-1317-64-2-255

Cited by 48 publications

(21 citation statements)

References 80 publications

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“…Recombinant adenovirus containing the transgene for either ␤-galactosidase (Ad-lacZ), Cu/Zn-SOD (Ad-SOD 1 ) or Mn-SOD (Ad-SOD 2 ) was prepared by the UNC vector core as described elsewhere. 38 Briefly, the plasmid shuttle vector pAd5.CMV.lacZ was constructed by standard cloning protocols as described by Graham and Prevec. 39 The adenoviral shuttle plasmid was transfected into the permissive HEK 293 host cell line to generate recombinant Ad-lacZ adenovirus.…”

Section: Animalsmentioning

confidence: 99%

Viral gene delivery of superoxide dismutase attenuates experimental cholestasis-induced liver fibrosis in the rat

et al. 2002

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Section: Animalsmentioning

confidence: 99%

Viral gene delivery of superoxide dismutase attenuates experimental cholestasis-induced liver fibrosis in the rat

et al. 2002

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“…Recombinant DNA technology has made possible the expression of heterologous genes in a variety of animal viruses (for review, see Rigby, 1983). The availability of a wide variety of animal virus vectors enables a foreign gene to be expressed in different cell types, at different levels and with different consequences for the host cell.…”

Section: Introductionmentioning

confidence: 99%

Vaccinia Virus Expression Vectors

Mackett

Smith

1986

Journal of General Virology

172

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“…17 was constructed as described elsewhere. 18,19 Briefly, the insert of the plasmid pRc/CMV-IB␣ S32A/S36A, which contains a hemagglutinin (HA)-tagged human superrepressor of NF-B, was subcloned into the Xba1 site of the pACCMV.PLPASR (ϩ) plasmid to construct the plasmid pACCMV/IB. IB superrepressor synthesis is driven by the cytomegalovirus-promoter/enhancer.…”

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confidence: 99%

Delivery of I[kappa ]B superrepressor gene with adenovirus reduces early alcohol-induced liver injury in rats

et al. 2001

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Chronic alcohol administration increases gut-derived endotoxin in the portal blood, which activates Kupffer cells through nuclear factor B (NF-B) to produce toxic mediators such as proinflammatory cytokines, leading to liver injury. Therefore, a long-term intragastric ethanol feeding protocol was used here to test the hypothesis that NF-B inhibition would prevent early alcohol-induced liver injury. Adenoviral vectors encoding either the transgene for IB superrepressor (AdIB-SR) or the bacterial ␤-galactosidase reporter gene (AdlacZ) were administered intravenously to Wistar rats. Animals were fed a high-fat liquid diet with either ethanol or isocaloric maltose-dextrin (control) for 3 weeks. There was no significant difference in mean urine alcohol concentrations between the groups fed ethanol. IB-SR expression was increased for up to 2 weeks after injection, but was undetectable at 3 weeks. NF-B activation was increased by ethanol and associated with up-regulation of tumor necrosis factor ␣ (TNF-␣). These increases were blunted significantly up to 2 weeks by AdIB-SR. Dietary alcohol significantly increased liver to body weight ratios and serum alanine transaminase (ALT) levels in AdlacZtreated animals, effects that were blunted significantly in AdIB-SR-treated rats. Ethanol caused severe steatosis, inflammation, and focal necrosis in AdlacZ-treated animals. These pathologic changes were significantly decreased by AdIB-SR. The protective effects of IB-SR were significant 2 weeks after injection, but were lost at 3 weeks when IB-SR was no longer expressed. Ethanol increased 4-hydroxynonenal as a maker of oxidative stress in both AdlacZ and AdIB groups. These data support the hypothesis that NF-B inhibition prevents early alcohol-induced liver injury even in the presence of oxidative stress. (HEPATOLOGY 2001; 34:1149-1157.)

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Cloning Vectors Derived from Animal Viruses

Cited by 48 publications

References 80 publications

Viral gene delivery of superoxide dismutase attenuates experimental cholestasis-induced liver fibrosis in the rat

Viral gene delivery of superoxide dismutase attenuates experimental cholestasis-induced liver fibrosis in the rat

Vaccinia Virus Expression Vectors

Delivery of I[kappa ]B superrepressor gene with adenovirus reduces early alcohol-induced liver injury in rats

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