Cloning, tissue distribution and effects of fasting on mRNA expression levels of leptin and ghrelin in red-bellied piranha (Pygocentrus nattereri)

Volkoff, Hélène

doi:10.1016/j.ygcen.2015.05.004

Cited by 36 publications

(25 citation statements)

References 76 publications

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“…The cck mRNA levels decrease by fasting in different fish species (Murashita et al, 2006;Feng et al, 2012;Ji et al, 2015), conversely ghrelin increase mRNA levels by fasting (Amole & Unniappan, 2009;Zhou & Xue, 2009;Tian et al, 2015;Volkoff, 2015;Blanco et al, 2016). These gene expression modulations were not observed in our results.…”

Section: Discussioncontrasting

confidence: 73%

Comparison of gene expression patterns of key growth genes between different rate growths in zebrafish (Danio rerio) siblings

Opazo¹,

Valladares²,

Romero³

2017

lajar

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ABSTRACT. Variable individual growth rate is a phenomenon observed in fish cohorts that influences the aquaculture performance and fish cohort ecological viability. Our aim was to compare gene expression patterns of key growth genes in zebrafish larvae with different growth rate. The body length of sibling zebrafish larvae at 6 days post hatching (dph) was measured. The larvae were reared to 20 dph and measured again. Two bodylength groups were clearly observed: 4 mm (small larvae) and 5-6 mm (large larvae). Total RNA was isolated from both groups. Growth hormone (gh), growth hormone receptor (ghr), insulin-like growth factor 1 (igf-1), insulin-like growth factor receptor (igf-1r a/b), insulin-like growth factor binding protein 1 (igfbp-1), thyroglobulin (tg), cholecystokinin (cck), and ghrelin were evaluated by quantitative polymerase chain reaction (qPCR). Glucokinase (gck) and igfbp-1 were included as a gene expression marker of larvae nutritional status. Two genes showed significant differences between the body length groups, igfbp-1 (P = 0.01) and igf-1r (P = 0.02). The igfbp-1 suggests than growth rate variability was associated with the larvae nutritional status and this condition affect the gene expression pattern of igf-1r. Therefore these genes are interesting genes markers for growth rate variabilities studies.

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Section: Discussioncontrasting

confidence: 73%

Comparison of gene expression patterns of key growth genes between different rate growths in zebrafish (Danio rerio) siblings

Opazo¹,

Valladares²,

Romero³

2017

lajar

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“…Using goldfish as a model, Unniappan et al provided the first fish ghrelin cDNA sequence and the first evidence of an orexigenic role for ghrelin in fish, as central injections of ghrelin stimulated food intake (Unniappan et al, 2002). Subsequent studies on several fish species reported sequences for ghrelin and confirmed its role as an appetite stimulator in fish (see Jönsson, 2013 for a review), including other Cypriniformes [e.g., goldfish (Kang et al, 2011; Nisembaum et al, 2014; Blanco et al, 2016a); gibel carp ( Carassius auratus gibelio ) (Zhou et al, 2016); Schizothorax davidi (Zhou et al, 2014)], Characiformes (red-bellied piranha Volkoff, 2015b), Perciformes (Nile tilapia Schwandt et al, 2010), for which fasting-induced and periprandial changes in expression/protein levels occur. In Salmoniformes, there is contradictory evidence.…”

Section: Hormones Involved In Food Intakementioning

confidence: 87%

“…In goldfish, no significant differences in either brain or liver leptin expressions are seen between control, overfed and fasting fish, suggesting nutritional status does not affect the leptin system in goldfish (Tinoco et al, 2012). Similarly, leptin expression is not affected by fasting in the liver of common carp (Huising et al, 2006a) (Cyrpiniforme) and Nile tilapia (Shpilman et al, 2014) (Perciforme) or in the brains of red-bellied piranha (Volkoff, 2015b) and pacu (Volkoff et al, 2017) (Characiformes). However, fasting/food restriction increases hepatic leptin expression in white-clouds mountain minnow ( Tanichthys albonubes , Cypriniforme; Chen et al, 2016b), in most Perciformes examined (orange-spotted grouper Zhang et al, 2013, mandarin fish Yuan et al, 2016, and mackerel Scomber japonicus Ohga et al, 2015, European sea bass Gambardella et al, 2012), in Arctic charr (Jørgensen et al, 2013) and Atlantic salmon (Rønnestad et al, 2010; Trombley et al, 2012; Moen and Finn, 2013) (Salmoniformes).…”

Section: Hormones Involved In Food Intakementioning

confidence: 99%

The Neuroendocrine Regulation of Food Intake in Fish: A Review of Current Knowledge

2016

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Fish are the most diversified group of vertebrates and, although progress has been made in the past years, only relatively few fish species have been examined to date, with regards to the endocrine regulation of feeding in fish. In fish, as in mammals, feeding behavior is ultimately regulated by central effectors within feeding centers of the brain, which receive and process information from endocrine signals from both brain and peripheral tissues. Although basic endocrine mechanisms regulating feeding appear to be conserved among vertebrates, major physiological differences between fish and mammals and the diversity of fish, in particular in regard to feeding habits, digestive tract anatomy and physiology, suggest the existence of fish- and species-specific regulating mechanisms. This review provides an overview of hormones known to regulate food intake in fish, emphasizing on major hormones and the main fish groups studied to date.

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“…The architecture of the orexin system (gene sequence, cell bodies, fibres and receptors) in the brain of fishes exhibits a general pattern that is common to all studied groups of vertebrates (Wong et al 2011, López et al 2014, Volkoff 2015a). The orexin system regulates neural activities that are responsible for coordinating daily functions across a range of taxa from fish to mammals, such as feeding behaviour, energy balance, locomotor activity and the sleep-wake cycle (reviewed in Matsuda et al 2012, Gao & Hermes 2015.…”

Section: Orexinmentioning

confidence: 99%

“…It has been implicated in the stimulation of growth hormone release from the pituitary, feeding and metabolism regulation and reproduction, among other functions, in both mammals (Al Massadi et al 2015, Müller et al 2015 and fishes (Kang et al 2011, Shahjahan et al 2014. As in other vertebrates, ghrelin in fishes is mainly synthesised in the intestinal tract, as well as in the brain (Kaiya et al 2011, Eom et al 2013, Zhou et al 2014, Ji et al 2015, Volkoff 2015a.…”

Section: Ghrelinmentioning

confidence: 99%

Interplay between the endocrine and circadian systems in fishes

Isorna

Pedro

Valenciano

et al. 2017

Journal of Endocrinology

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The circadian system is responsible for the temporal organisation of physiological functions which, in part, involves daily cycles of hormonal activity. In this review, we analyse the interplay between the circadian and endocrine systems in fishes. We first describe the current model of fish circadian system organisation and the basis of the molecular clockwork that enables different tissues to act as internal pacemakers. This system consists of a net of central and peripherally located oscillators and can be synchronised by the light-darkness and feeding-fasting cycles. We then focus on two central neuroendocrine transducers (melatonin and orexin) and three peripheral hormones (leptin, ghrelin and cortisol), which are involved in the synchronisation of the circadian system in mammals and/or energy status signalling. We review the role of each of these as overt rhythms (i.e. outputs of the circadian system) and, for the first time, as key internal temporal messengers that act as inputs for other endogenous oscillators. Based on acute changes in clock gene expression, we describe the currently accepted model of endogenous oscillator entrainment by the light-darkness cycle and propose a new model for non-photic (endocrine) entrainment, highlighting the importance of the bidirectional cross-talking between the endocrine and circadian systems in fishes. The flexibility of the fish circadian system combined with the absence of a master clock makes these vertebrates a very attractive model for studying communication among oscillators to drive functionally coordinated outputs.

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Cloning, tissue distribution and effects of fasting on mRNA expression levels of leptin and ghrelin in red-bellied piranha (Pygocentrus nattereri)

Cited by 36 publications

References 76 publications

Comparison of gene expression patterns of key growth genes between different rate growths in zebrafish (Danio rerio) siblings

Comparison of gene expression patterns of key growth genes between different rate growths in zebrafish (Danio rerio) siblings

The Neuroendocrine Regulation of Food Intake in Fish: A Review of Current Knowledge

Interplay between the endocrine and circadian systems in fishes

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