Cloning the double-stranded RNA genes of reovirus: sequence of the cloned S2 gene.

supporting

confidence: 71%

Rotavirus RNA Segments Sized by Electron Microscopy

Rixon

Taylor

Desselberger

1984

“…A genomic cDNA library from porcine C60 rotavirus was constructed using pUC 13 as cloning vector. In brief, total viral genomic dsRNA was 3' polyadenylated with E. coli poly(A) polymerase (Both et al, 1982;Cashdollar et al, 1982) and reverse transcribed by priming with oligo(dT) (Maniatis et al, 1982). The second cDNA strand was synthesized according to the method of Gubler & Hoffman (1983) and the ds cDNAs were blunt-end ligated to SmaI-cleaved and dephosphorylated pUC13.…”

Section: Cells and Virusesmentioning

confidence: 99%

Structure of Rearranged Genome Segment 11 in Two Different Rotavirus Strains Generated by a Similar Mechanism

González

Mattion²,

Bellinzoni³

et al. 1989

SUMMARYThe structures of the rearranged genomic segment l 1 of two spontaneous swine rotavirus strains were determined. We found that the rearrangements involved the duplication of normal segment 11 in a head-to-tail orientation, and partial deletions in both monomers. The open reading frame for VP11, the protein encoded by normal segment ll, was maintained. We also showed that the two rearranged genes were transcribed into RNA molecules of the same length as their corresponding genomic segments.

“…The cDNA library of RGDV genome segments was made by the method of Cashdollar et al (1982). All dsRNA of RGDV was incubated in 90~ DMSO at 50 °C for 45 min, and then polyadenylated using poly(A) polymerase (Bethesda Research Laboratories).…”

mentioning

confidence: 99%

Nucleotide Sequence of Segment S9 of the Genome of Rice Gall Dwarf Virus

Koganezawa

Hibino²,

Motoyoshi

et al. 1990

DNA complementary to the ninth largest ($9) of the 12 genome segments of rice gall dwarf virus (RGDV) was cloned and its sequence was determined. It is 1202 nucleotides in length and contains one open reading frame which extends for 969 nucleotides from nucleotide 26. It encodes a polypeptide of 323 amino acids with an M~ of 35 560. The dinucleotide sequence at the 5' end and the trinucleotide sequence at the 3' end of the plus strand, 5' GG--GAU 3', which are present in the RNA of both wound tumour virus (WTV) and rice dwarf virus (RDV), were also found in RGDV genome segment $9. The nucleotide sequences in the noncoding region at the 5' terminus and in the 15 nucleotides at the 3' terminus, which form an imperfect inverted repeat of 10 bp together with the 5' terminus, are approximately 70% homologous with those of the WTV genome segment $9, but only 30% and 50% homologous with the respective termini of RDV $9.