Cloning, Sequencing, and Disruption of the Gene Encoding Sterol C-14 Reductase in <i>Saccharomyces cerevisiae</i>

Lorenz, R T; Parks, Leo W.

doi:10.1089/dna.1992.11.685

Cited by 66 publications

(50 citation statements)

References 10 publications

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“…We assume that delivery of newly synthesized proteins to appropriate locations, e.g., the bud neck, cell surface, or cell wall, is diminished at high pressure and low temperature when one of the genes listed in Table 1 is lost. ERG24 encodes the C-14 sterol reductase that catalyzes a step in ergosterol biosynthesis (Lorenz and Parks 1992). The erg24 mutants are viable but accumulate the abnormal sterol ignosterol (ergosta-8, 14 dienol) (Parks et al 1995).…”

Section: Resultsmentioning

confidence: 99%

Global Screening of Genes Essential for Growth in High-Pressure and Cold Environments: Searching for Basic Adaptive Strategies Using a Yeast Deletion Library

Abe

Minegishi

2008

Genetics

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Microorganisms display an optimal temperature and hydrostatic pressure for growth. To establish the molecular basis of piezo-and psychroadaptation, we elucidated global genetic defects that give rise to susceptibility to high pressure and low temperature in Saccharomyces cerevisiae. Here we present 80 genes including 71 genes responsible for high-pressure growth and 56 responsible for low-temperature growth with a significant overlap of 47 genes. Numerous previously known cold-sensitive mutants exhibit marked high-pressure sensitivity. We identified critically important cellular functions: (i) amino acid biosynthesis, (ii) microautophagy and sorting of amino acid permease established by the exit from rapamycin-induced growth arrest/Gap1 sorting in the endosome (EGO/GSE) complex, (iii) mitochondrial functions, (iv) membrane trafficking, (v) actin organization mediated by Drs2-Cdc50, and (vi) transcription regulated by the Ccr4-Not complex. The loss of EGO/GSE complex resulted in a marked defect in amino acid uptake following high-pressure and low-temperature incubation, suggesting its role in surface delivery of amino acid permeases. Microautophagy and mitochondrial functions converge on glutamine homeostasis in the target of rapamycin (TOR) signaling pathway. The localization of actin requires numerous associated proteins to be properly delivered by membrane trafficking. In this study, we offer a novel route to gaining insights into cellular functions and the genetic network from growth properties of deletion mutants under high pressure and low temperature.

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Section: Resultsmentioning

confidence: 99%

Global Screening of Genes Essential for Growth in High-Pressure and Cold Environments: Searching for Basic Adaptive Strategies Using a Yeast Deletion Library

Abe

Minegishi

2008

Genetics

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“…15 Morpholine treatment leads to the production of ergosta-8,14-dienol (ignosterol), which cannot support growth under normal conditions. Cloning and disruption 133,148,158 of the ERG24 gene indicated that it was essential, but conflicting evidence was provided as to whether growth inhibition was due to the accumulation of non-utilizable 8,14-dien sterols 148 or the lack of ergosterol. 158 Non-viability resulting from the elimination of the ERG24 gene product is also subject to suppression in yeast.…”

Section: Sterol Biosynthesis In Yeast: Farnesyl Pyrophosphate To Ergomentioning

confidence: 99%

Biochemistry, cell biology and molecular biology of lipids ofSaccharomyces cerevisiae

Daum

Lees

Bard

et al. 1998

Yeast

579

380

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The yeast Saccharomyces cerevisiae is a powerful experimental system to study biochemical, cell biological and molecular biological aspects of lipid synthesis. Most but not all genes encoding enzymes involved in fatty acid, phospholipid, sterol or sphingolipid biosynthesis of this unicellular eukaryote have been cloned, and many gene products have been functionally characterized. Less information is available about genes and gene products governing the transport of lipids between organelles and within membranes, turnover and degradation of complex lipids, regulation of lipid biosynthesis, and linkage of lipid metabolism to other cellular processes. Here we summarize current knowledge about lipid biosynthetic pathways in S. cerevisiae and describe the characteristic features of the gene products involved. We focus on recent discoveries in these fields and address questions on the regulation of lipid synthesis, subcellular localization of lipid biosynthetic steps, cross‐talk between organelles during lipid synthesis and subcellular distribution of lipids. Finally, we discuss distinct functions of certain key lipids and their possible roles in cellular processes. © 1998 John Wiley & Sons, Ltd.

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“…Through a BLASTP search at the Fusarium Comparative Database site (available at http://www.broadinstitute.org/ annotation/genome/fusarium_graminearum/MultiHome.html) using the amino acid sequence of S. cerevisiae sterol C-14 reductase Erg24 (Lorenz & Parks, 1992) as a query, we identified two Erg24-homologous sequences in F. graminearum, FGSG_06606.3 and FGSG_02346.3; these were named FgERG24A and FgERG24B, respectively. Transcripts of the two FgERG24 genes in F. graminearum were detected using genomic DNA and cDNA as templates with primer pairs Aspanintron-F+A-spanintron-R and B-spanintron-F+B-spanintron-R (Table 1), respectively.…”

Section: Methodsmentioning

confidence: 99%

A sterol C-14 reductase encoded by FgERG24B is responsible for the intrinsic resistance of Fusarium graminearum to amine fungicides

Liu

Yun

et al. 2011

Microbiology

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Fusarium graminearum, the causal agent of wheat head blight, shows intrinsic resistance to amine fungicides. It is commonly accepted that the amines target sterol C-14 reductase and sterol D 8 -D 7 isomerase of ergosterol biosynthesis, encoded by the genes ERG24 and ERG2, respectively. Analysis of the genome sequence of F. graminearum revealed that the fungus contains two paralogous FgERG24 genes (FgERG24A and FgERG24B), which are homologous to the ERG24 of Saccharomyces cerevisiae. In this study, we disrupted FgERG24A and FgERG24B in F. graminearum. Compared to the wild-type strain HN9-1, FgERG24A and FgERG24B deletion mutants did not show recognizable phenotypic changes in mycelial growth on potato dextrose agar or in virulence on wheat heads. HPLC analysis showed that the amount of ergosterol in FgERG24A or FgERG24B deletion mutants was not significantly different from that in the wild-type strain. These results indicate that neither of the two genes is essential for growth, pathogenicity or ergosterol biosynthesis in F. graminearum. FgERG24B deletion mutants exhibited significantly increased sensitivity to amine fungicides, including tridemorph, fenpropidin and spiroxamine, but not to non-amine fungicides. In contrast, FgERG24A deletion mutants did not show changed sensitivity to any amine tested. The resistance of the FgERG24B deletion mutant to amines was restored by genetic complementation of the mutant with wild-type FgERG24B. These results indicate that FgERG24B controls the intrinsic resistance of F. graminearum to amines. The finding of this study provides new insights into amine resistance in filamentous fungi.

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Cloning, Sequencing, and Disruption of the Gene Encoding Sterol C-14 Reductase in Saccharomyces cerevisiae

Cited by 66 publications

References 10 publications

Global Screening of Genes Essential for Growth in High-Pressure and Cold Environments: Searching for Basic Adaptive Strategies Using a Yeast Deletion Library

Global Screening of Genes Essential for Growth in High-Pressure and Cold Environments: Searching for Basic Adaptive Strategies Using a Yeast Deletion Library

Biochemistry, cell biology and molecular biology of lipids ofSaccharomyces cerevisiae

A sterol C-14 reductase encoded by FgERG24B is responsible for the intrinsic resistance of Fusarium graminearum to amine fungicides

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