Cloning, prokaryotic expression of rice L-galactono-1,4-lactone dehydrogenase gene and preparation of anti-GLDH antibodies

Yu, Lei; Liu, Yonghai; XinXiang, Peng

doi:10.3724/sp.j.1238.2010.00381

Cited by 1 publication

(2 citation statements)

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“…Rubisco protein and Chl content were positively correlated with the Asc content. Asc had protective effects on photosynthetic organs and increased biomass by minimizing the loss of photosynthestic performance (Yabuta et al 2002, Yu et al 2010. The results showed that the Rubisco protein and Chl content of the leaves of three different plants decreased after treatment at HT, while the increase in Asc reduced the degradation of Rubisco protein and Chl.…”

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confidence: 93%

“…---It has been reported that l-galactono-1,4-lactone dehydrogenase (GLDH) was the rate-limiting enzyme in Asc synthesis (Smirnoff et al 2001), and the Asc content in plants was positively correlated with the expression of GLDH gene (Landi et al 2015, Zhang et al 2016, Tokunaga et al 2005. GLDH up-regulated and the GLDH down-regulated rice strains had been successfully constructed (Yu et al 2010(Yu et al , 2017.…”

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confidence: 99%

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Effects of endogenous ascorbic acid on resistance to high-temperature stress in excised rice leaves

2018

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Ascorbic acid (Asc) is a major plant antioxidant. L-galactono-1,4-lactone dehydrogenase (GLDH) is an enzyme that catalyzes the last step of Asc biosynthesis in higher plants. Effects of endogenous Asc on resistance to high-temperature stress were studied by using GLDH-overexpressed (GO-2) and GLDH-suppressed transgenic rice (GI-2) as experimental materials. After high-temperature treatment, the maximal quantum yield of PSII was significantly lower in GI-2, and higher in GO-2 compared to wild type rice. The content of reactive oxygen species (ROS) was the highest in GI-2. The higher Asc content resulted in lower lipid peroxidation in GO-2. The contents of chlorophyll, soluble proteins, and Rubisco large and small subunit were positively correlated to the Asc content. These results show that the higher Asc content reduced the accumulation of ROS and maintained the function of rice leaves. We suggest that the higher Asc content could improve the rice resistance to high-temperature stress.

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confidence: 93%