Cloning of zebrafish Mustn1 orthologs and their expression during early development

Promoter architecture and transcriptional regulation of musculoskeletal embryonic nuclear protein 1b (mustn1b) gene in zebrafish

“…At 3 dpf, eGFP expression was also found in craniofacial muscles and pectoral fins. Therefore, transgenic reporter lines expressing eGFP under the Zebrafish 0.8 kb mustn1b promoter and flanking successfully demonstrate the recapitulation of developmental expression of mustn1b gene (Camarata et al, 2016). The temporal activation of the mustn1b promoter is also consistent with the timing of the activation of endogenous mustn1b gene during normal development.…”

“…Upstream from the ATG start site, a TATA box and consensus sequences for potential transcription factor binding were identified and the minimal promoter region determined. Therefore, transgenic reporter lines expressing eGFP under the Zebrafish 0.8 kb mustn1b promoter and flanking successfully demonstrate the recapitulation of developmental expression of mustn1b gene (Camarata et al, 2016). The constructs analyzed consisted of the eGFP reporter gene driven by three different lengths of mustn1b promoter regions.…”

“…It has been shown in amphibians that mustn1 knockdown causes craniofacial cartilage malformations as well as reduced expression of sox9 in Xenopus embryos, supporting its importance for in vivo chondrogenesis as well (Gersch et al, 2012). Its expression was detected in the Kupffer's vesicle, paraxial mesoderm, and otic vesicle, however expression in heart and musculoskeletal tissues was restricted to Zebrafish mustn1b (Camarata et al, 2016). Its expression was detected in the Kupffer's vesicle, paraxial mesoderm, and otic vesicle, however expression in heart and musculoskeletal tissues was restricted to Zebrafish mustn1b (Camarata et al, 2016).…”

“…In fish, the embryonic and larval developmental expression of mustn1 co-orthologs (i.e., mustn1a and mustn1b) has recently been reported (Camarata et al, 2016). Its expression was detected in the Kupffer's vesicle, paraxial mesoderm, and otic vesicle, however expression in heart and musculoskeletal tissues was restricted to Zebrafish mustn1b (Camarata et al, 2016). Furthermore, loss-of-function studies using Morpholinos showed the involvement of Zebrafish mustn1a in the cilia formation (Choksi et al, 2014).…”

Mustn1: A Developmentally Regulated Pan-Musculoskeletal Cell Marker and Regulatory Gene

“…Lastly, we recently reported on the cloning of zebrafish mustn1 . Interestingly, we identified two orthologs, mustn1 a and mustn1 b, that shared 71% homology at the amino acid level and whose predicted proteins were highly related to other vertebrate members (63% to human, 61% to frog and chimp, 60% to dog, chicken and cow, 56% to mouse, and 54% to rat) [ 12 ]. The discovery and comparative sequence analyses of the vertebrate Mustn1 gene have given rise to a new protein family, labeled “ Mustang ” (after the original name we assigned to this gene [ 5 ]) as outlined by UnitPro ( , access date: 12 January 2017).…”