Cloning of wheat keto-acyl thiolase 2B reveals a role of jasmonic acid in grain weight determination

Chen, Yun; Yan, Yan; Wu, Tiantian; Zhang, Guo-Liang; Yin, Huanran; Chen, Wei; Wang, Shuangshuang; Chang, Fang Rong; Gou, Jin‐Ying

doi:10.1038/s41467-020-20133-z

Cited by 45 publications

(29 citation statements)

References 52 publications

(57 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The grain-shape gene TasgD1 encoding a Ser/Thr protein kinase glycogen synthase kinase3 and independently control semispherical grain trait [ 13 ]. A jasmonic acid synthetic gene keto-acyl thiolase 2B was cloned in a TGW mutant, showing significant effects on TGW and GW [ 14 ]. Additionally, homologous cloning is an effective approach to characterize gene in wheat.…”

Section: Introductionmentioning

confidence: 99%

High-resolution detection of quantitative trait loci for seven important yield-related traits in wheat (Triticum aestivum L.) using a high-density SLAF-seq genetic map

Wang

et al. 2022

BMC Genom Data

View full text Add to dashboard Cite

Background Yield-related traits including thousand grain weight (TGW), grain number per spike (GNS), grain width (GW), grain length (GL), plant height (PH), spike length (SL), and spikelet number per spike (SNS) are greatly associated with grain yield of wheat (Triticum aestivum L.). To detect quantitative trait loci (QTL) associated with them, 193 recombinant inbred lines derived from two elite winter wheat varieties Chuanmai42 and Chuanmai39 were employed to perform QTL mapping in six/eight environments. Results A total of 30 QTLs on chromosomes 1A, 1B, 1D, 2A, 2B, 2D, 3A, 4A, 5A, 5B, 6A, 6D, 7A, 7B and 7D were identified. Among them, six major QTLs QTgw.cib-6A.1, QTgw.cib-6A.2, QGw.cib-6A, QGl.cib-3A, QGl.cib-6A, and QSl.cib-2D explaining 5.96-23.75% of the phenotypic variance were detected in multi-environments and showed strong and stable effects on corresponding traits. Three QTL clusters on chromosomes 2D and 6A containing 10 QTLs were also detected, which showed significant pleiotropic effects on multiple traits. Additionally, three Kompetitive Allele Specific PCR (KASP) markers linked with five of these major QTLs were developed. Candidate genes of QTgw.cib-6A.1/QGl.cib-6A and QGl.cib-3A were analyzed based on the spatiotemporal expression patterns, gene annotation, and orthologous search. Conclusions Six major QTLs for TGW, GL, GW and SL were detected. Three KASP markers linked with five of these major QTLs were developed. These QTLs and KASP markers will be useful for elucidating the genetic architecture of grain yield and developing new wheat varieties with high and stable yield in wheat.

show abstract

Section: Introductionmentioning

confidence: 99%

High-resolution detection of quantitative trait loci for seven important yield-related traits in wheat (Triticum aestivum L.) using a high-density SLAF-seq genetic map

Wang

et al. 2022

BMC Genom Data

View full text Add to dashboard Cite

show abstract

“…Because of the complexity of wheat genome, genetic research of wheat yield–related traits and their components were mainly focused on QTL mapping and molecular marker development. Recently, numerous QTLs/genes for yield-related traits in wheat have been identified on almost 21 wheat chromosomes ( Cui et al, 2014 , 2016 ; Huang et al, 2015 ; Wu et al, 2015 ; Brinton et al, 2017 ; Cao et al, 2019 ; Sakuma et al, 2019 ; Chen et al, 2020 ; Cheng et al, 2020 ; Ren et al, 2021 ). However, the effects of QTL in hexaploid wheat were usually subtle than those identified in rice, and few environmental-stable QTLs have been identified and validated ( Cristobal, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Genetic Mapping and Validation of Loci for Kernel-Related Traits in Wheat (Triticum aestivum L.)

Liu

Xie

et al. 2021

Front. Plant Sci.

View full text Add to dashboard Cite

Kernel size (KS) and kernel weight play a key role in wheat yield. Phenotypic data from six environments and a Wheat55K single-nucleotide polymorphism array–based constructed genetic linkage map from a recombinant inbred line population derived from the cross between the wheat line 20828 and the line SY95-71 were used to identify quantitative trait locus (QTL) for kernel length (KL), kernel width (KW), kernel thickness (KT), thousand-kernel weight (TKW), kernel length–width ratio (LWR), KS, and factor form density (FFD). The results showed that 65 QTLs associated with kernel traits were detected, of which the major QTLs QKL.sicau-2SY-1B, QKW.sicau-2SY-6D, QKT.sicau-2SY-2D, and QTKW.sicau-2SY-2D, QLWR.sicau-2SY-6D, QKS.sicau-2SY-1B/2D/6D, and QFFD.sicau-2SY-2D controlling KL, KW, KT, TKW, LWR, KS, and FFD, and identified in multiple environments, respectively. They were located on chromosomes 1BL, 2DL, and 6DS and formed three QTL clusters. Comparison of genetic and physical interval suggested that only QKL.sicau-2SY-1B located on chromosome 1BL was likely a novel QTL. A Kompetitive Allele Specific Polymerase chain reaction (KASP) marker, KASP-AX-109379070, closely linked to this novel QTL was developed and used to successfully confirm its effect in two different genetic populations and three variety panels consisting of 272 Chinese wheat landraces, 300 Chinese wheat cultivars most from the Yellow and Huai River Valley wheat region, and 165 Sichuan wheat cultivars. The relationships between kernel traits and other agronomic traits were detected and discussed. A few predicted genes involved in regulation of kernel growth and development were identified in the intervals of these identified major QTL. Taken together, these stable and major QTLs provide valuable information for understanding the genetic composition of kernel yield and provide the basis for molecular marker–assisted breeding.

show abstract

“…These data indicate that TaSTT3b‐2B promoted grain weight most possibly by affecting the expression of starch synthase and sucrose synthase genes. In a most recent article, in wheat, mutation of the JA synthetic gene, KAT‐2B , produces smaller grains and accumulates less JA, while overexpression of KAT‐2B increases grain weight and yield in plants grown in field trials (Chen et al ., 2020 ). Rice mutants defective in JA biosynthesis ( dfo2 and Osopr7 ) or signaling ( Osmyc2 ) exhibited various degrees of abnormality in spikelet development, including abnormal number or morphology of lemma, palea, glume, lodicule, and floral organs, which can be partially rescued by application of exogenous JA (You et al ., 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Overexpression of TaSTT3b‐2B improves resistance to sharp eyespot and increases grain weight in wheat

Zhu

Rong

Wang

et al. 2021

Plant Biotechnology Journal

View full text Add to dashboard Cite

STAUROSPORINE AND TEMPERATURE SENSITIVE3 (STT3) is a catalytic subunit of oligosaccharyltransferase, which is important for asparagine-linked glycosylation. Sharp eyespot, caused by the necrotrophic fungal pathogen Rhizoctonia cerealis, is a devastating disease of bread wheat. However, the molecular mechanisms underlying wheat defense against R. cerealis are still largely unclear. In this study, we identified TaSTT3a and TaSTT3b, two STT3 subunit genes from wheat and reported their functional roles in wheat defense against R. cerealis and increasing grain weight. The transcript abundance of TaSTT3b-2B was associated with the degree of wheat resistance to R. cerealis and induced by both R. cerealis and exogenous jasmonic acid (JA). Overexpression of TaSTT3b-2B significantly enhanced resistance to R. cerealis, grain weight, and JA content in transgenic wheat subjected to R. cerealis stress, while silencing of TaSTT3b-2B compromised resistance of wheat to R. cerealis. Transcriptomic analysis showed that TaSTT3b-2B affected the expression of a series of defense-related genes and JA biosynthesis-related genes, as well as genes coding starch synthase and sucrose synthase. Application of exogenous JA elevated expression levels of the abovementioned defense-and grain weight-related genes, and rescuing the resistance of TaSTT3b-2B-silenced wheat to R. cerealis, while pretreatment with sodium diethyldithiocarbamate, an inhibitor of JA synthesis, attenuated the TaSTT3b-2B-mediated resistance to R. cerealis, suggesting that TaSTT3b-2B played critical roles in regulating R. cerealis resistance and grain weight via JA biosynthesis. Altogether, this study reveals new functional roles of TaSTT3b-2B in regulating plant innate immunity and grain weight, and illustrates its potential application value for wheat molecular breeding.

show abstract

Cloning of wheat keto-acyl thiolase 2B reveals a role of jasmonic acid in grain weight determination

Cited by 45 publications

References 52 publications

High-resolution detection of quantitative trait loci for seven important yield-related traits in wheat (Triticum aestivum L.) using a high-density SLAF-seq genetic map

High-resolution detection of quantitative trait loci for seven important yield-related traits in wheat (Triticum aestivum L.) using a high-density SLAF-seq genetic map

Genetic Mapping and Validation of Loci for Kernel-Related Traits in Wheat (Triticum aestivum L.)

Overexpression of TaSTT3b‐2B improves resistance to sharp eyespot and increases grain weight in wheat

Contact Info

Product

Resources

About