Cloning of three estrogen receptors (ER) from killifish (Fundulus heteroclitus): Differences in populations from polluted and reference environments

Greytak, Sarah R.; Callard, Gloria V.

doi:10.1016/j.ygcen.2006.07.017

Cited by 66 publications

(78 citation statements)

References 58 publications

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“…In addition, Atlantic Wood offspring were more susceptible to mortality associated with infectious disease (Frederick, Van Veld, & Rice, 2007), to toxicity associated with photoactivation of PAHs, and to acute hypoxia (Meyer & Di Giulio, 2003), although they were less susceptible to the model pro‐oxidant tert ‐butyl hydroperoxide than reference fish (Meyer, Smith, Winston, & Di Giulio, 2003). Altered estrogen signaling has been observed in DLC‐tolerant killifish from both Newark and NBH (Bugel et al., 2011, 2014; Greytak & Callard, 2007; Greytak et al., 2010). NBH killifish are more sensitive to the pro‐oxidant chemical tert ‐butylhydroquinone as compared to fish from a reference site (Harbeitner, Hahn, & Timme‐Laragy, 2013); however, the opposite effect was seen in ER fish, which were more tolerant of the oxidant tert ‐butylhydroperoxide than reference fish (Meyer et al., 2003).…”

Section: Ecological Considerationsmentioning

confidence: 99%

When evolution is the solution to pollution: Key principles, and lessons from rapid repeated adaptation of killifish (Fundulus heteroclitus) populations

Whitehead

Clark

Reid

et al. 2017

Evolutionary Applications

120

102

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For most species, evolutionary adaptation is not expected to be sufficiently rapid to buffer the effects of human‐mediated environmental changes, including environmental pollution. Here we review how key features of populations, the characteristics of environmental pollution, and the genetic architecture underlying adaptive traits, may interact to shape the likelihood of evolutionary rescue from pollution. Large populations of Atlantic killifish (Fundulus heteroclitus) persist in some of the most contaminated estuaries of the United States, and killifish studies have provided some of the first insights into the types of genomic changes that enable rapid evolutionary rescue from complexly degraded environments. We describe how selection by industrial pollutants and other stressors has acted on multiple populations of killifish and posit that extreme nucleotide diversity uniquely positions this species for successful evolutionary adaptation. Mechanistic studies have identified some of the genetic underpinnings of adaptation to a well‐studied class of toxic pollutants; however, multiple genetic regions under selection in wild populations seem to reflect more complex responses to diverse native stressors and/or compensatory responses to primary adaptation. The discovery of these pollution‐adapted killifish populations suggests that the evolutionary influence of anthropogenic stressors as selective agents occurs widely. Yet adaptation to chemical pollution in terrestrial and aquatic vertebrate wildlife may rarely be a successful “solution to pollution” because potentially adaptive phenotypes may be complex and incur fitness costs, and therefore be unlikely to evolve quickly enough, especially in species with small population sizes.

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Section: Ecological Considerationsmentioning

confidence: 99%

When evolution is the solution to pollution: Key principles, and lessons from rapid repeated adaptation of killifish (Fundulus heteroclitus) populations

Whitehead

Clark

Reid

et al. 2017

Evolutionary Applications

120

102

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“…Both CYP19 genes have been previously cloned in Fundulus (Greytak et al, 2005;Patel et al, 2006). Fundulus have been used as an environmentally relevant toxicology model organism to study endocrine disruption (Boudreau et al, 2005;Dube and MacLatchy 2001;Greytak and Callard 2007;Kelly and Di Giulio 2000), environmental carcinogenesis and polycyclic aromatic hydrocarbon (PAH) toxicity (Billiard et al, 2006;Vogelbein et al, 1990;, and chemically mediated changes in gene expression (Meyer et al, 2005;Paschall et al, 2004;Powell et al, 2000). The embryonic developmental stages of Fundulus have been described (Armstrong and Child 1965).…”

Section: Introductionmentioning

confidence: 99%

Local expression of CYP19A1 and CYP19A2 in developing and adult killifish (Fundulus heteroclitus)

Wang¹,

Willett²

2008

General and Comparative Endocrinology

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P450 aromatase (CYP19) is the terminal enzyme in the steroidogenic pathway and catalyzes the conversion of androgens to estrogens. Fundulus heteroclitus like other teleosts, express two CYP19 genes, CYP19A1 and CYP19A2. The expression of CYP19s in Fundulus was measured by in situ hybridization throughout development. In 90 dpf (day post-fertilization) fish and adult fish, CYP19A1 was expressed in the ooplasm of early stage I oocytes (primary growth stage). Expression of CYP19A1 was localized in the follicle cell layer of late stage I (previtellogenic stage) and stage II (vitellogenic stage) follicles, but by stage III (early maturational follicles) CYP19A1 expression was localized in the vitelline envelope. Overall, CYP19A1 oocyte membrane expression gradually declined from highest expression at late stage I to nondetectable levels by stage IV. Highest expression of CYP19A2 was detected in the brain including the hypothalamus from 4, 6, 8, 10, 14 dpf embryos, 90 dpf fry fish and adult fish brain. In females compared to males, there was higher CYP19A2 expression in olfactory bulb. In addition to the brain, there was strong CYP19A2 signal in adrenal/kidney cells in 6-14 dpf embryos. This work establishes the localization and constitutive expression of CYP19s in Fundulus which can then be compared with potential disruption of CYP19A1 and CYP19A2 expression and physiological consequences caused by environmental contaminants.

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“…Among these, the estrogen receptors (ERs; NR3A subfamily) have received the most attention. F. heteroclitus, like other fishes, differs from mammals in having two ERβ paralogs (ERβa and ERβb) in addition to a single ERα (Urushitani et al, 2003;Greytak and Callard, 2007). Evidence that these ERs are involved in toxic and adaptive responses to contaminant exposure is discussed below (Toxicology).…”

Section: Gene Regulationmentioning

confidence: 99%

“…Furthermore, biomarkers of xenobiotic exposure such as CYP1A inducibility are measurable early in the embryonic period and can be quantified using traditional measures of enzyme activity (e.g., Binder and Stegeman, 1980) or immunodetectable CYP1A protein (Van Veld et al, 1992Toomey et al, 2001). Similarly, the predominant brain form of aromatase CYP19B mRNA is a sensitive and robust biomarker of environmental estrogen in both adult and larval killifish (Greytak et al, 2005;Greytak and Callard, 2007). In addition, novel non-invasive methods have been developed for quantifying the activity of contaminantinducible embryonic enzymes to assess variation in sensitivity to contaminants among embryos and populations (Nacci et al, 1998Arzuaga et al, 2006).…”

Section: Toxicologymentioning

confidence: 99%

“…End-points used to assess reproductive condition of sexually mature adults include reproductive success, circulating sex steroids, gonadal size, hepatic size, yolk protein synthesis, and genetic markers such as CYP19 aromatases, ER and vitellogenin (Black et al, 1998a,b;Monteverdi and Di Giulio, 2000;Matta et al, 2001;MacLatchy et al, 2003;Boudreau et al, 2004;Boudreau et al, 2005;Greytak et al, 2005;Greytak and Callard, 2007).…”

Section: Reproductive and Developmental Effectsmentioning

confidence: 99%

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Fundulus as the premier teleost model in environmental biology: Opportunities for new insights using genomics

Burnett

Bain

Baldwin

et al. 2007

Comparative Biochemistry and Physiology Part D: Genomics and Pr

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213

195

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A strong foundation of basic and applied research documents that the estuarine fish Fundulus heteroclitus and related species are unique laboratory and field models for understanding how individuals and populations interact with their environment. In this paper we summarize an extensive body of work examining the adaptive responses of Fundulus species to environmental conditions, and describe how this research has contributed importantly to our understanding of physiology, gene regulation, toxicology, and ecological and evolutionary genetics of teleosts and other vertebrates. These explorations have reached a critical juncture at which advancement is hindered by the lack of genomic resources for these species. We suggest that a more complete genomics toolbox for F. heteroclitus and related species will permit researchers to exploit the power of this model organism to rapidly advance our understanding of fundamental biological and pathological mechanisms among vertebrates, as well as ecological strategies and evolutionary processes common to all living organisms.

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Cloning of three estrogen receptors (ER) from killifish (Fundulus heteroclitus): Differences in populations from polluted and reference environments

Cited by 66 publications

References 58 publications

When evolution is the solution to pollution: Key principles, and lessons from rapid repeated adaptation of killifish (Fundulus heteroclitus) populations

When evolution is the solution to pollution: Key principles, and lessons from rapid repeated adaptation of killifish (Fundulus heteroclitus) populations

Local expression of CYP19A1 and CYP19A2 in developing and adult killifish (Fundulus heteroclitus)

Fundulus as the premier teleost model in environmental biology: Opportunities for new insights using genomics

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