Cloning of the ω-secalin gene family in a wheat 1BL/1RS translocation line using BAC clone sequencing

Li, Meng Jun; Li, Ya Qing; Zhang, Nan; Shi, Zhan Liang

doi:10.1016/j.ejbt.2015.12.001

Cited by 2 publications

(2 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although these secalins have been found to negatively affect gluten, dough and end-use properties by many genetic and rheological studies (Barbeau et al 2003;Dhaliwal et al 1990), the precise copy numbers of γ-and ω-secalin genes carried by Sec-1 are still not well understood, neither is it clear how many distinct γ-and ω-secalins are accumulated in the grains of 1BL/1RS wheat varieties. Nevertheless, three studies have estimated the size of Sec-1 to be at least 145 kb or 195 kb and contained 15 or 18 ω-secalin genes (Clarke et al 1996;Li et al 2016;Yamamoto and Mukai, 2005). The transcripts for 17 different ω-secalin genes were detected in the developing grains of the 1BL/1RS variety Shimai 15 by PCR cloning of cDNAs (Li et al 2016).…”

Section: Proteomic Insight Into Gluten Protein Accumulation In Wheat Grainsmentioning

confidence: 99%

“…Nevertheless, three studies have estimated the size of Sec-1 to be at least 145 kb or 195 kb and contained 15 or 18 ω-secalin genes (Clarke et al 1996;Li et al 2016;Yamamoto and Mukai, 2005). The transcripts for 17 different ω-secalin genes were detected in the developing grains of the 1BL/1RS variety Shimai 15 by PCR cloning of cDNAs (Li et al 2016). Four ω-secalin protein bands were detected in a SDS-PAGE analysis of 14 1BL/1RS cultivars (Chai et al…”

Section: Proteomic Insight Into Gluten Protein Accumulation In Wheat Grainsmentioning

confidence: 99%

See 1 more Smart Citation

Genomic and functional genomics analyses of gluten proteins and prospect for simultaneous improvement of end-use and health-related traits in wheat

Wang

Cao

et al. 2020

Theor Appl Genet

View full text Add to dashboard Cite

Key message Recent genomic and functional genomics analyses have substantially improved the understanding on gluten proteins, which are important determinants of wheat grain quality traits. The new insights obtained and the availability of precise, versatile and high-throughput genome editing technologies will accelerate simultaneous improvement of wheat end-use and health-related traits. Abstract Being a major staple food crop in the world, wheat provides an indispensable source of dietary energy and nutrients to the human population. As worldwide population grows and living standards rise in both developed and developing countries, the demand for wheat with high quality attributes increases globally. However, efficient breeding of high-quality wheat depends on critically the knowledge on gluten proteins, which mainly include several families of prolamin proteins specifically accumulated in the endospermic tissues of grains. Although gluten proteins have been studied for many decades, efficient manipulation of these proteins for simultaneous enhancement of end-use and health-related traits has been difficult because of high complexities in their expression, function and genetic variation. However, recent genomic and functional genomics analyses have substantially improved the understanding on gluten proteins. Therefore, the main objective of this review is to summarize the genomic and functional genomics information obtained in the last 10 years on gluten protein chromosome loci and genes and the cis- and trans-factors regulating their expression in the grains, as well as the efforts in elucidating the involvement of gluten proteins in several wheat sensitivities affecting genetically susceptible human individuals. The new insights gathered, plus the availability of precise, versatile and high-throughput genome editing technologies, promise to speed up the concurrent improvement of wheat end-use and health-related traits and the development of high-quality cultivars for different consumption needs.

show abstract

Section: Proteomic Insight Into Gluten Protein Accumulation In Wheat Grainsmentioning

confidence: 99%

Section: Proteomic Insight Into Gluten Protein Accumulation In Wheat Grainsmentioning

confidence: 99%

Genomic and functional genomics analyses of gluten proteins and prospect for simultaneous improvement of end-use and health-related traits in wheat

Wang

Cao

et al. 2020

Theor Appl Genet

View full text Add to dashboard Cite

show abstract

Bridging the Genotype–Phenotype Gap for Precision Breeding in Rye

Hackauf

Rabanus‐Wallace

Korzun

2021

Compendium of Plant Genomes

View full text Add to dashboard Cite

Cloning of the ω-secalin gene family in a wheat 1BL/1RS translocation line using BAC clone sequencing

Cited by 2 publications

References 11 publications

Genomic and functional genomics analyses of gluten proteins and prospect for simultaneous improvement of end-use and health-related traits in wheat

Genomic and functional genomics analyses of gluten proteins and prospect for simultaneous improvement of end-use and health-related traits in wheat

Bridging the Genotype–Phenotype Gap for Precision Breeding in Rye

Contact Info

Product

Resources

About