Cloning of the pcyA gene from Arthrospira platensis FACHB314 and its function on expression of a fluorescent phycocyanin in heterologous hosts

Sun, Deguang; Xiao, Dongfang; Zang, Xiaonan; Wu, Fei; Jin, Yuming; Cao, Xuexue; Guo, Yalin; Li, Zhu; Wang, Haitao; Zhang, Xuecheng

doi:10.1007/s10811-019-01865-y

Cited by 5 publications

(4 citation statements)

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“…To construct the most suitable PCB synthetic strain for C. glutamicum, we selected strains known for their high natural production of PCB among many candidates and compared their HO1 and PcyA enzymes. − …”

Section: Results and Discussionmentioning

confidence: 99%

“…glutamicum, Ho1 from A. platensis, and Ho1 from Synechocystis sp. ,, The effects of each enzyme were analyzed based on the SDS-PAGE and mRNA expression levels. Based on the mRNA expression level, the Ho1 derived from Synechocystis sp.…”

Section: Results and Discussionmentioning

confidence: 99%

“…platensis, PcyA from Synechocystis sp., and codon-optimized PcyA from Synechocystis sp. Each enzyme was analyzed using SDS-PAGE and mRNA expression levels. , SDS-PAGE analysis revealed that PcyA from Synechocystis sp. exhibited the highest enzyme expression (Figure A).…”

Section: Results and Discussionmentioning

confidence: 99%

“…Each enzyme was analyzed using SDS-PAGE and mRNA expression levels. 33,34 SDS-PAGE analysis revealed that PcyA from Synechocystis sp. exhibited the highest enzyme expression (Figure 3A).…”

Section: Screening Of Pcya From Various Strains and Construction Of T...mentioning

confidence: 99%

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Enhancing Phycocyanobilin Production Efficiency in Engineered Corynebacterium glutamicum: Strategies and Potential Application

Chang,

Shi,

Kim

et al. 2024

J. Agric. Food Chem.

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Phycocyanobilin, an algae-originated light-harvesting pigment known for its antioxidant properties, has gained attention as it plays important roles in the food and medication industries and has surged in demand owing to its low-yield extraction from natural resources. In this study, engineered Corynebacterium glutamicum was developed to achieve high PCB production, and three strategies were proposed: reinforcement of the heme biosynthesis pathway with the introduction of two PCB-related enzymes, strengthening of the pentose phosphate pathway to generate an efficient cycle of NADPH, and fed-batch fermentation to maximize PCB production. Each approach increased PCB synthesis, and the final engineered strain successfully produced 78.19 mg/L in a flask and 259.63 mg/L in a 5 L bioreactor, representing the highest bacterial production of PCB reported to date, to our knowledge. The strategies applied in this study will be useful for the synthesis of PCB derivatives and can be applied in the food and pharmaceutical industries.

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Section: Results and Discussionmentioning

confidence: 99%

Section: Results and Discussionmentioning

confidence: 99%

Section: Results and Discussionmentioning

confidence: 99%

“…Each enzyme was analyzed using SDS-PAGE and mRNA expression levels. 33,34 SDS-PAGE analysis revealed that PcyA from Synechocystis sp. exhibited the highest enzyme expression (Figure 3A).…”

Section: Screening Of Pcya From Various Strains and Construction Of T...mentioning

confidence: 99%

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