Cloning of the nodulation (nod) genes of Rhizobium phaseoli and their homology to R. leguminosarum nod DNA

Lamb, J. W.; Downie, J. Allan; Johnston, Andrew

doi:10.1016/0378-1119(85)90132-5

Cited by 37 publications

(9 citation statements)

References 27 publications

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“…The range of melanin production in rhizobia has been characterized elsewhere (9), although its biological significance in the Rhizobium-legume symbiosis, if any, is not well understood. Loci involved in melanin production have been identified in different locations, either on symbiotic plasmids (3,20,21) or on cryptic plasmids (18), for several strains. Melanin biosynthesis in strain 8002 of Rhizobium leguminosarum bv.…”

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confidence: 99%

Melanin production by Rhizobium meliloti GR4 is linked to nonsymbiotic plasmid pRmeGR4b: cloning, sequencing, and expression of the tyrosinase gene mepA

et al. 1993

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Melanin production by Rhizobium meliloti GR4 is linked to nonsymbiotic plasmid pRmeGR4b (140 MDa). Transfer of this plasmid to GR4-cured derivatives or to Agrobacterium tumefaciens enables these bacteria to produce melanin. Sequence analysis of a 3.5-kb PstI fragment of plasmid pRmeGR4b has revealed the presence of a open reading frame 1,481-bp that codes for a protein whose sequence shows strong homology to two conserved regions involved in copper binding in tyrosinases and hemocyanins. In vitro-coupled transcription-translation experiments showed that this open reading frame codes for a 55-kDa polypeptide. Melanin production in GR4 is not under the control of the RpoN-NifA regulatory system, unlike that in R. leguminosarum bv. phaseoli 8002. The GR4 tyrosinase gene could be expressed in Escherichia coli under the control of the lacZ promoter. For avoiding confusion with mel genes (for melibiose), a change of the name of the previously reported mel genes of R. leguminosarum bv. phaseoli and other organisms to mep genes (for melanin production) is proposed.

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confidence: 99%

Melanin production by Rhizobium meliloti GR4 is linked to nonsymbiotic plasmid pRmeGR4b: cloning, sequencing, and expression of the tyrosinase gene mepA

et al. 1993

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“…They were eventually isolated from all of the major groups of rhizobia, including A. caulinodans (Van den Eede et al, 1987;Goethals et al, 1990), Bradyrhizobium spp. (Marvel et al, 1985;Noti et al, 1985;Russell et al, 1985;Lamb and Hennecke, 1986;Hahn and Hennecke, 1988;Deshmane and Stacey, 1989;Gottfert et al, 1989Gottfert et al, , 1992Dobert et al, 1904), R. etli (Cevallos et al, 1989;Vhzquez et al, 1991), R. fredii (Ramakrishnan et al, 1986;Appelbaum et al, 1988;Krishnan and Pueppke, 1991b), R. galegae (Rasanen et al, 1991), R. leguminosarum bv.phaseoli (Lamb et al, 1985;Davis and Johnston, 1990a), R. tropici (Vargas et al, 1990), and broad host range Rhizobium sp. NGR234 or its derivative MPIK3030 (Bachem et al, 1985;Bassam et al, 1986).…”

Section: Isolation Of Nod Genesmentioning

confidence: 93%

The Genetic and Biochemical Basis for Nodulation of Legumes by Rhizobia

Pueppke

1996

Critical Reviews in Biotechnology

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Soil bacteria of the genera Azorhizobium, Bradyrhizobium, and Rhizobium are collectively termed rhizobia. They share the ability to penetrate legume roots and elicit morphological responses that lead to the appearance of nodules. Bacteria within these symbiotic structures fix atmosphere nitrogen and thus are of immense ecological and agricultural significance. Although modern genetic analysis of rhizobia began less than 20 years ago, dozens of nodulation genes have now been identified, some in multiple species of rhizobia. These genetic advances have led to the discovery of a host surveillance system encoded by nodD and to the identification of Nod factor signals. These derivatives of oligochitin are synthesized by the protein products of nodABC, nodFE, NodPQ, and other nodulation genes; they provoke symbiotic responses on the part of the host and have generated immense interest in recent years. The symbiotic functions of other nodulation genes are nonetheless uncertain, and there remain significant gaps in our knowledge of several large groups of rhizobia with interesting biological properties. This review focuses on the nodulation genes of rhizobia, with particular emphasis on the concept of biological specificity of symbiosis with legume host plants.

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“…pIJ1518 were constructed by cloning 1.7 Kb BcII fragment of pRL1JI into pKT230, containing an intact nodD gene expressed from vector promoter (21). The TSPs (transcription start point) for nodF, px 2 , nodD and nodA on the pIJ1518 were indicated, and the locations of primers used in this work were illustrated.…”

Section: Figmentioning

confidence: 99%

px2, the Newly Identified Gene in Rhizobium leguminosarum, Is Characterized to Enhance Its Adjacent nodF Expression

Yang

Hong

2000

Biochemical and Biophysical Research Communications

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Cloning of the nodulation (nod) genes of Rhizobium phaseoli and their homology to R. leguminosarum nod DNA

Cited by 37 publications

References 27 publications

Melanin production by Rhizobium meliloti GR4 is linked to nonsymbiotic plasmid pRmeGR4b: cloning, sequencing, and expression of the tyrosinase gene mepA

Melanin production by Rhizobium meliloti GR4 is linked to nonsymbiotic plasmid pRmeGR4b: cloning, sequencing, and expression of the tyrosinase gene mepA

The Genetic and Biochemical Basis for Nodulation of Legumes by Rhizobia

px2, the Newly Identified Gene in Rhizobium leguminosarum, Is Characterized to Enhance Its Adjacent nodF Expression

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