Cloning of the gene encoding a 50 kilodalton potential surface antigen of Schistosoma mansoni

Havercroft, Jane C.; Huggins, Maureen C.; Nene, Vishvanath; Dunne, David W.; Richardson, Brian A.; Taylor, David W.; Butterworth, Anthony E.

doi:10.1016/0166-6851(88)90135-1

Cited by 14 publications

(11 citation statements)

References 24 publications

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“…Individual pre-and post-immunization sera were assayed for antibodies to Sm20, Sm50 and GST by Western blotting against adult S. munsoni worm antigen (Figure l), as previously described (Havercroft et al 1988;1990). Sm26 and Sj26 species show crossreactive epitopes, allowing us to test for responses to both the S. munsoni portion of our fusion proteins and the Sj26 carrier protein on the same Western blot.…”

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confidence: 99%

The influence of adjuvant on humoral responses to glutathione‐S‐transferase fusion proteins

Varley

Dunne

Havercroft

1992

Parasite Immunology

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Mice were immunized with one of two Schistosoma mansoni antigens, Sm20 and Sm50, expressed as fusion proteins with Schistosoma japonicum 26,000 Dalton glutathione-S-transferase (GST) or with GST alone. Antibody responses to GST were shown to be critically dependent on the adjuvant used. In Sm20-GST immunized mice, we noted a striking bias to respond either to Sm20 or to GST according to the adjuvant used and responses to both in the same individual were rare. Fusion with Sm50, which is relatively non-immunogenic, appeared to down-regulate responses to GST.

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confidence: 99%

The influence of adjuvant on humoral responses to glutathione‐S‐transferase fusion proteins

Varley

Dunne

Havercroft

1992

Parasite Immunology

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“…12/9 with GST or against GST alone by repeated subcutaneous immunization with approximately 100 fig of purified fusion protein in Freund's adjuvant (Life Technologies Ltd, Paisley). SDS-polyacrylamide gels and Western blots were prepared as previously described (Havercroft et al 1988). Parasite samples for SDS-polyacrylamide gel electrophoresis were prepared as before (Havercroft et al 1990;Havercroft, Smith & Williams, 1991).…”

Section: Western Blottingmentioning

confidence: 99%

“…Previously, we described a clone which encoded a portion of a 50 kDa antigen (Sm50) (Havercroft et al 1988) which was present on the surface of schistosomula of S. mansoni. This antigen could act as a target for the antibody-dependent killing of schistosomula by human eosinophils in vitro and thus represented a candidate antigen for the development of a genetically engineered vaccine for schistosomiasis.…”

Section: Introductionmentioning

confidence: 99%

Schistosoma mansoni: adult males and females differentially express antigens encoded by repetitive genomic DNA

Smith

Huggins²,

Havercroft³

1992

Parasitology

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Two repetitive DNA sequences have been characterized from Schistosoma mansoni which were transcribed into mRNAs and translated to give two families of cross-reactive proteins. One DNA element, which was present as a 230 bp Pst I fragment was arranged in tandem arrays of at least 17 copies in the genome. The second element, which could be localized to a 1800 bp Pst I fragment, was dispersed in the genome. The 1800 bp repeat was found on the mRNA encoding the 45 kDa polypeptide precursor of a potential surface antigen. This precursor was post-translationally modified to give a 50 kDa antigen (Sm50) which was expressed from the cercaria to the adult worm and in the egg. However, a proportion of this antigen was differentially modified in females and eggs to give a 60 kDa form. Two copies of the 230 bp repeat and one copy of the 1800 bp repeat were found on a second cDNA clone. The antiserum raised against the fusion protein of this clone recognized a family of cross-reactive proteins ranging from 14 to 70 kDa in size. The members of this family were also differentially expressed between the sexes. Consequently, two families of antigens have been identified which were both encoded by repetitive DNA elements and whose members were both differentially expressed in adult male and female worms.

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“…12 (Genbank accession number M94347), which contains a portion of the 1800 bp repetitive element only. A1.12 encodes a 45 kDa polypeptide precursor which is post-translationally modified, presumably by glycosylation, to give a 50 kDa antigen (Sm50) (Havercroft et a!. 1988) in all life cycle stages and an additional 60 kDa form in adult worms and eggs ).…”

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confidence: 99%

“…1988) in all life cycle stages and an additional 60 kDa form in adult worms and eggs ). Previously, we had shown, by indirect immuno-fluorescence, that Sm50 was expressed on the surface of schistosomula and that antibody directed against this antigen could mediate killing of schistosomula by human eosinophils in uitro (Havercroft et al 1988). In this paper we have used an antiserum against the related Al.…”

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confidence: 99%

Localization of the A1.12/9 antigen family to the neurones, putative sensory receptors and tegument of Schistosoma mansoni

Havercroft

Smith

1993

Parasite Immunology

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The A1.12/9 antigen family were localized to the periphery of vesicle-like structures in neurones and in the putative sensory receptors of cercariae by immuno-electron microscopy. After transformation into schistosomula, the antigens were rapidly lost until, after 18 h, no immunoreactivity could be detected. Expression resumed at approximately 36 h post-transformation and had returned to high levels by 4 days post-infection. This level was maintained through to the adult worm stage. In 4 day lung schistosomula and in 14 day and 21 day liver schistosomula immunolabelling was observed within the matrix of the tegument itself and also in association with the tegumental membrane and vesicles within the tegument. These properties suggest that the A1.12/9 antigens may be involved in neuropeptide processing pathways. In particular, this family may represent the schistosome homologue of the granin family with which they share common properties and some sequence homology.

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Cloning of the gene encoding a 50 kilodalton potential surface antigen of Schistosoma mansoni

Cited by 14 publications

References 24 publications

The influence of adjuvant on humoral responses to glutathione‐S‐transferase fusion proteins

The influence of adjuvant on humoral responses to glutathione‐S‐transferase fusion proteins

Schistosoma mansoni: adult males and females differentially express antigens encoded by repetitive genomic DNA

Localization of the A1.12/9 antigen family to the neurones, putative sensory receptors and tegument of Schistosoma mansoni

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