Cloning of the cDNA and functional expression of the 47-kilodalton cytosolic component of human neutrophil respiratory burst oxidase.

Volpp, Bryan D.; Nauseef, William M.; Donelson, John E.; Moser, David J.; Clark, Robert A.

doi:10.1073/pnas.86.18.7195

Cited by 287 publications

(145 citation statements)

References 38 publications

(38 reference statements)

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“…The oxidase subunit p47 phox contains several serine residues clustered within the carboxyl terminus that form potential protein kinase C phosphorylation sites (29). Native p47 phox is a highly basic protein that undergoes stepwise charge shifts with successive phosphorylation events (29,30).…”

Section: Discussionmentioning

confidence: 99%

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Mechanism of Endothelial Cell NADPH Oxidase Activation by Angiotensin II

Shah

2003

Journal of Biological Chemistry

276

189

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Section: Discussionmentioning

confidence: 99%

“…Native p47 phox is a highly basic protein that undergoes stepwise charge shifts with successive phosphorylation events (29,30). In neutrophils, p47 phox phosphorylation is thought to be a prerequisite for its interaction with p22 phox and the formation of a functional oxidase complex (15,16).…”

Section: Discussionmentioning

confidence: 99%

Mechanism of Endothelial Cell NADPH Oxidase Activation by Angiotensin II

Shah

2003

Journal of Biological Chemistry

276

189

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“…The ORF of human p47 phox cDNA (17,18) was inserted into the MFGS retrovirus vector (Cell Genesys, Foster City, CA) (8-10). MFGS-p47 phox plasmid was transfected into the amphotropic envelope packaging line -CRIP, and a vector-producing clone was selected (8)(9)(10).…”

mentioning

confidence: 99%

Prolonged production of NADPH oxidase-corrected granulocytes after gene therapy of chronic granulomatous disease

Malech

Maples

Whiting-Theobald

et al. 1997

Proc. Natl. Acad. Sci. U.S.A.

336

153

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Little is known about the potential for engraftment of autologous hematopoietic stem cells in human adults not subjected to myeloablative conditioning regimens. Five adult patients with the p47 phox deficiency form of chronic granulomatous disease received intravenous infusions of autologous CD34 ؉ peripheral blood stem cells (PBSCs) that had been transduced ex vivo with a recombinant retrovirus encoding normal p47 phox . Although marrow conditioning was not given, functionally corrected granulocytes were detectable in peripheral blood of all five patients. Peak correction occurred 3-6 weeks after infusion and ranged from 0.004 to 0.05% of total peripheral blood granulocytes. Corrected cells were detectable for as long as 6 months after infusion in some individuals. Thus, prolonged engraftment of autologous PBSCs and continued expression of the transduced gene can occur in adults without conditioning. This trial also piloted the use of animal protein-free medium and a blood-bankcompatible closed system of gas-permeable plastic containers for culture and transduction of the PBSCs. These features enhance the safety of PBSCs directed gene therapy.

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“…Cloning of the various components of the oxidase demonstrated that they contain SH3 domains and proline-rich regions that can interact with each other and are important in the assembly of the catalytically active oxidase at the plasma membrane (25)(26)(27). It was suggested that part of the activation process involves changes in the conformation of the various components, revealing new binding sites.…”

Section: Discussionmentioning

confidence: 99%

“…Phosphorylation or detergents such as SDS could provide the means for such conformation changes (28). Since (17,29,30). However, using various methodologies, several groups showed that PKC, cAMP protein kinase (PKA), the mitogen-activated protein kinases p38MAPK and ERK2, and the p21-activated kinases (PAKs) could phosphorylate p47Phox in vitro (10,(31)(32)(33).…”

Section: Discussionmentioning

confidence: 99%

Modulation of the Alveolar Macrophage Superoxide Production by Protein Phosphorylation

Forman¹,

Zhou²,

Gozal³

et al. 1998

Environmental Health Perspectives

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Stimulation of alveolar macrophages (AM) with adenosine-5-diphosphate (ADP) results in transient production of superoxide anion radical (02-; superoxide) and H202 in a metabolic event known as the respiratory burst. Initiation of the respiratory burst appears to depend on activation of protein kinase activity, whereas protein phosphatases might involved in termination of the burst. The involvement of protein kinase C was suggested by inhibition by bisindolylmaleimide (GF 109203X), a relatively specific inhibitor. KN-62, an inhibitor of calcium-calmodulin protein kinase 11, also partly inhibited the respiratory burst stimulated by ADP and phorbol esters. The role of protein phosphatases in termination of the ADP-stimulated respiratory burst of AM was examined with calyculin A (CA) (25-75 nM) or okadaic acid (OA) (11-5 pM), two inhibitors of protein phosphatase 1 and 2a (PP1;PP2a). A dose-dependent prolongation of the respiratory burst was observed in the presence of these inhibitors. CA and OA also markedly enhanced the rate of superoxide production stimulated by ADP, consistent with involvement of PP1/PP2a in regulating both the rate of activation and timing of termination. Treatment of AM with cyclosporin A (CsA) (1-50 pM), an inhibitor of the calcium-dependent protein phosphatase 2b (PP2b), stimulated superoxide production by itself and significantly prolonged the duration of ADP-stimulated superoxide production. CsA, however, did not increase the ADP-stimulated rate of superoxide production. Thus, PP1/PP2a appear to be the primary phosphatases for controlling the intensity of the respiratory burst during receptor-elicited superoxide production in AM, whereas PP1/PP2a and PP2b play a role in turning off the respiratory burst. Environ Health Perspect 1 06(Suppl 5):1185-1190 (1998). http://ehpnetl.niehs.nih.gov/docs/1998/Suppl-5/ 1185-1 190forman/abstract.html

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Cloning of the cDNA and functional expression of the 47-kilodalton cytosolic component of human neutrophil respiratory burst oxidase.

Abstract: 9563Correction. In the article "Cloning of the cDNA and functional expression of the 47-kilodalton cytosolic component of human neutrophil respiratory burst oxidase"

Cited by 287 publications

References 38 publications

Mechanism of Endothelial Cell NADPH Oxidase Activation by Angiotensin II

Mechanism of Endothelial Cell NADPH Oxidase Activation by Angiotensin II

Prolonged production of NADPH oxidase-corrected granulocytes after gene therapy of chronic granulomatous disease

Modulation of the Alveolar Macrophage Superoxide Production by Protein Phosphorylation

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