Cloning of the Brcer1 gene involved in cuticular wax production in a glossy mutant of non-heading Chinese cabbage (Brassica rapa L. var. communis)

Wang, Canjie; Li, Yixiao; Xie, Fei; Kuang, Hanhui; Wan, Zhengjie

doi:10.1007/s11032-017-0745-2

Cited by 13 publications

(9 citation statements)

References 45 publications

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“…Four of these genes were identified in our selective sweep and differential gene expression analyses. Mapping studies previously identified these genes as being associated with leaf color variation ( Bra006208 ; Fu et al ., 2019), cuticular wax biosynthesis ( Bra011470 and Bra032670 ; Wang et al ., 2017, 2019), and clubroot resistance ( Bra019410 ; Yu et al ., 2016). For other loci, mapping studies have identified a small collection of candidate genes in target regions.…”

Section: Resultsmentioning

confidence: 99%

Genes derived from ancient polyploidy have higher genetic diversity and are associated with domestication in Brassica rapa

Hall

et al. 2021

New Phytologist

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Summary Many crops are polyploid or have a polyploid ancestry. Recent phylogenetic analyses have found that polyploidy often preceded the domestication of crop plants. One explanation for this observation is that increased genetic diversity following polyploidy may have been important during the strong artificial selection that occurs during domestication. In order to test the connection between domestication and polyploidy, we identified and examined candidate genes associated with the domestication of the diverse crop varieties of Brassica rapa. Like all ‘diploid’ flowering plants, B. rapa has a diploidized paleopolyploid genome and experienced many rounds of whole genome duplication (WGD). We analyzed transcriptome data of more than 100 cultivated B. rapa accessions. Using a combination of approaches, we identified > 3000 candidate genes associated with the domestication of four major B. rapa crop varieties. Consistent with our expectation, we found that the candidate genes were significantly enriched with genes derived from the Brassiceae mesohexaploidy. We also observed that paleologs were significantly more diverse than non‐paleologs. Our analyses find evidence for that genetic diversity derived from ancient polyploidy played a key role in the domestication of B. rapa and provide support for its importance in the success of modern agriculture.

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Section: Resultsmentioning

confidence: 99%

Genes derived from ancient polyploidy have higher genetic diversity and are associated with domestication in Brassica rapa

Hall

et al. 2021

New Phytologist

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“…In our study, Bra032642 (CYP86A4), Bra029852 (CYP77A6), Bra003862 (HTH), and Bra026140 (WIN1/ SHINE1) were all up-regulated in the G-bulk, which indicated enhanced cutin biosynthesis. Previous studies have shown that the cutin in glossy plants is thicker than that in waxy plants (Wang et al 2017;Pu et al 2013), and the Fig. 5 Transcriptome analysis in waxy and glossy stems.…”

Section: Discussionmentioning

confidence: 99%

BrWAX2 plays an essential role in cuticular wax biosynthesis in Chinese cabbage (Brassica rapa L. ssp. pekinensis)

et al. 2021

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Key message Map-based cloning was used to identify the BrWAX2 gene, which was involved in the cuticular wax biosynthesis. The malfunction of BrWAX2 together with other reduced expression of genes in alkane-forming pathway caused the glossy phenotype. Abstract Cuticular wax covering the outer plant surface plays various roles in protecting against biotic and abiotic stresses. Wax-less mutant shows glossy in stem and leaf surface and plays important roles in enriching Chinese cabbage germplasm resources for breeding brilliant green varieties. However, genes responsible for the glossy trait in Chinese cabbage are rarely reported. In this study, we identified a glossy Chinese cabbage line Y1211-1. Genetic analysis indicated that the glossy trait in Y1211-1 was controlled by a single recessive locus, BrWAX2 (Brassica rapa WAX 2). Using bulked segregant sequencing (BSA-Seq) and kompetitive allele-specific PCR (KASP) assays, BrWAX2 was fine-mapped to an interval of 100.78 kb. Functional annotation analysis, expression analysis, and sequence variation analysis revealed that Bra032670, homologous to CER1 in Arabidopsis, was the most likely candidate gene for BrWAX2. The gene Bra032670 was absent in glossy mutant. Cuticular wax composition analysis and RNA-Seq analysis suggested that the absence of BrWAX2 together with the decreased expression of other genes in alkane-forming pathway reduced the wax amount and caused the glossy phenotype. Furthermore, we developed and validated the functional marker BrWAX2-sp for BrWAX2. Overall, these results provide insight into the molecular mechanism underlying cuticular wax biosynthesis and reveal valuable information for marker-assisted selection (MAS) breeding in Chinese cabbage.

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“…The results showed that DEGs were mainly enriched in 11 KEGG pathways, including plant hormone signal transduction, phenylpropane biosynthesis, phenylalanine and α‐linolenic acid metabolism, β‐alanine metabolism, and cutin, suberin, and wax synthesis (Figure S8b). Previous studies have shown that the genes related to cutin, suberin, and wax synthesis can regulate plant glossiness and that wax and cutin can also affect vegetable glossiness (Girard et al., 2012; Lee et al., 2009; Li et al., 2014; Petit et al., 2016; Wang et al., 2017). We found that the relative expression of genes related to wax and cutin synthesis was mediated but showed an irregular pattern (Figure 5a); however, the relative expression of the main genes related to wax and cutin transport was upregulated (Figure 5b).…”

Section: Resultsmentioning

confidence: 99%

The COPII subunit CsSEC23 mediates fruit glossiness in cucumber

et al. 2023

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SUMMARYTo improve our understanding of the mechanism underlying cucumber glossiness regulation, a novel cucumber mutant with a glossy peel (Csgp) was identified. MutMap, genotyping, and gene editing results demonstrated that CsSEC23, which is the core component of COPII vesicles, mediates the glossiness of cucumber fruit peel. CsSEC23 is functionally conserved and located in the Golgi and endoplasmic reticulum. CsSEC23 could interact with CsSEC31, but this interaction was absent in the Csgp mutant, which decreased the efficiency of COPII vesicle transportation. Genes related to wax and cutin transport were upregulated in the Csgp mutant, and the cuticle structure of the Csgp‐mutant peel became thinner. Moreover, the wax and cutin contents were also changed due to CsSEC23 mutation. Taken together, the results obtained from this study revealed that CsSEC23 mediates cucumber glossiness, and this mediating might be affected by COPII vesicle transportation.

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Cloning of the Brcer1 gene involved in cuticular wax production in a glossy mutant of non-heading Chinese cabbage (Brassica rapa L. var. communis)

Cited by 13 publications

References 45 publications

Genes derived from ancient polyploidy have higher genetic diversity and are associated with domestication in Brassica rapa

Genes derived from ancient polyploidy have higher genetic diversity and are associated with domestication in Brassica rapa

BrWAX2 plays an essential role in cuticular wax biosynthesis in Chinese cabbage (Brassica rapa L. ssp. pekinensis)

The COPII subunit CsSEC23 mediates fruit glossiness in cucumber

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