1984
DOI: 10.1099/00221287-130-5-1271
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Cloning of the Aspartase Gene (aspA) of Escherichia coli

Abstract: The aspartase gene (aspA) of Escherichiu coli has been isolated in two plasmids, pGS73 and pGS94, which contain segments of bacterial DNA (12.5 and 2.8 kb, respectively) inserted into the tet gene of the vector pBR322. The plasmids were constructed by sequential sub-cloning from a larger ColE1-frd+ hybrid plasmid. The location of the aspA gene confirmed predictions based on a correlation between the genetic and restriction maps of the corresponding region. The aspartase activities of plasmid-containing aspA mu… Show more

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Cited by 19 publications
(15 citation statements)
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References 26 publications
(27 reference statements)
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“…The aspA gene encoding aspartase has been cloned and sequenced from several different bacterial sources (Guest et al, 1984;Takagi and Kisumi, 1985;Takagi et al, 1986a;Sun and Setlow, 1991). The aspA gene in E. coli encodes a 478 amino acid protein with a subunit molecular weight of 52 kDa , and the catalytically active enzyme is a tetramer composed of identical subunits (Williams and Lartigue, 1967).…”
Section: E Sequence Homologymentioning
confidence: 99%
“…The aspA gene encoding aspartase has been cloned and sequenced from several different bacterial sources (Guest et al, 1984;Takagi and Kisumi, 1985;Takagi et al, 1986a;Sun and Setlow, 1991). The aspA gene in E. coli encodes a 478 amino acid protein with a subunit molecular weight of 52 kDa , and the catalytically active enzyme is a tetramer composed of identical subunits (Williams and Lartigue, 1967).…”
Section: E Sequence Homologymentioning
confidence: 99%
“…coli cannot ferment glycerol anaerobically and requires the presence of a suitable electron acceptor such as fumarate for growth on this substrate (18). Asparagine can be converted to aspartate by asparaginase (4) and thence to the alternative electron acceptor fumarate by aspartase (14). Fumarate could be replaced by either of these amino acids for the anaerobic growth of wild-type E. coli on glycerol (Table 5).…”
Section: Methodsmentioning
confidence: 99%
“…Of those with a known function, yfiA (translational stimulator ; Bylund et al, 1997), tufA\tufB (EF-Tu ; reviewed by Weijland et al, 1992) and yjbC (putative pseudouridylate synthase, shows similarity to rsuA ; Wrzesinski et al, 1995) encode proteins involved in translation ; this adaptation may comprise a general response to stress or may merely be a function of lower growth rates in the presence of a toxic agent. In addition, tnaA (tryptophanase ; Deeley & Yanofsky, 1981) and aspA (aspartase ; Guest et al, 1984) are reduced, suggesting the cell is less catabolic in nature.…”
Section: Transcriptional Analysis Of Adapted Strainsmentioning
confidence: 99%