Cloning of the acetylcholinesterase 1 gene and identification of point mutations putatively associated with carbofuran resistance in Nilaparvata lugens

Point mutations in acetylcholinesterase 1 associated with chlorpyrifos resistance in the brown planthopper, Nilaparvata lugens Stål

“…Recombinant NlAChE1 showed a V max 15.9 times higher and a catalytic efficiency 4.4 times higher than NlAChE2. In insecticide‐resistant individuals of N. lugens , resistance‐associated mutations have only been observed in NlAChE1, although the roles of these mutations in insecticide resistance have not been evaluated in detail (Kwon et al ., ). In the present study, three mutations (G119S, F331C and I332L) in NlAChE1 were detected in individuals resistant to chlorpyrifos, and these mutations were subsequently confirmed as being associated with chlorpyrifos resistance based on target‐site insensitivity data from recombinant proteins.…”

“…Point mutations in AChE1 have been found to be associated with insecticide resistance in Hemiptera such as aphids, rice planthoppers and whiteflies (Nabeshima et al ., ; Li & Han, ; Alon et al ., ; Kwon et al ., ). In N. lugens , four mutations (G119A, F/Y330S, F331H and I332L) were identified in NlAChE1 and these mutations were believed to be associated with resistance to carbofuran, a carbamate insecticide (Kwon et al ., ). In the present study, similar mutations (G119S, F331C and I332L) at three loci of NlAChE1 were detected in a laboratory‐selected, chlorpyrifos‐resistant population, and the roles of these mutations in chlorpyrifos resistance were evaluated by comparing NlAChE1 wild‐type and mutants in vitro .…”

Biochemistry and molecular characterisation of chlorpyrifos resistance in field strains of the white‐backed planthopper Sogatella furcifera (Hemiptera: Delphacidae)

“…Mutations in the AChE gene (especially AChE1) have been widely shown in several insect species (Kwon et al 2012;Temeyer et al 2013;Zhang et al 2013) and have been associated with OP and CB resistance in D. melanogaster, C. pipiens, N. lugens, B. tabaci, C. tritaeniorhynchus, A. gambiae, Tetranychus evansi and P. xylostella (Fournier et al 1989;Weill et al 2002;Baek et al 2005;Oh et al 2006;Alon et al 2008;Alout et al 2009;Jiang et al 2009;Wang et al 2009;Carvalho et al 2012;Kwon et al 2012). Mutations in the AChE gene (especially AChE1) have been widely shown in several insect species (Kwon et al 2012;Temeyer et al 2013;Zhang et al 2013) and have been associated with OP and CB resistance in D. melanogaster, C. pipiens, N. lugens, B. tabaci, C. tritaeniorhynchus, A. gambiae, Tetranychus evansi and P. xylostella (Fournier et al 1989;Weill et al 2002;Baek et al 2005;Oh et al 2006;Alon et al 2008;Alout et al 2009;Jiang et al 2009;Wang et al 2009;Carvalho et al 2012;Kwon et al 2012).…”

“…In this study, we identified the potential target genes of OP and CB insecticides as SfAChE1 and SfAChE2 (Kwon et al 2012;Zhang et al 2013). We obtained full-length cDNA sequences cloned from WBPH and studied their expression patterns during distinct development stages and in different tissues.…”

“…Mutations in AChE have been hypothesised to be the mechanism that confers OP resistance to insects and have been reported in many insect species (Jiang et al 2009;Kwon et al 2012;Zhang et al 2013). Mutations in AChE have been hypothesised to be the mechanism that confers OP resistance to insects and have been reported in many insect species (Jiang et al 2009;Kwon et al 2012;Zhang et al 2013).…”