Cloning of TCFL5 encoding a novel human basic helix-loop-helix motif protein that is specifically expressed in primary spermatocytes at the pachytene stage

Maruyama, Osamu; Nishimori, Hiroyuki; Katagiri, Toyomasa; Miki, Y; Ueno, Akira; Nakamura, Yusuke

doi:10.1159/000015061

Cited by 27 publications

(29 citation statements)

References 16 publications

(30 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Also during spermatogenesis, TCFL5 is expressed in a highly cell-type and stage-specific pattern. 43,44 By overexpressing TCFL5 in FTOC, we obtained initial evidence that TCFL5 positively regulates T-cell development. The observed effects were mild, presumably because TCFL5 is expressed already during the early stages of T-cell development (Figure 5a), and enhanced expression is not likely to induce remarkable changes.…”

Section: Discussionmentioning

confidence: 99%

“…43 It was first found in spermatocytes, but was not detected in the thymus by Northern-blot analysis. 43,44 This might be explained by the fact that TCFL5 levels decrease in the more mature stages of T-cell development (Figure 5a), which comprise the larger part of the thymus. Also during spermatogenesis, TCFL5 is expressed in a highly cell-type and stage-specific pattern.…”

Section: Discussionmentioning

confidence: 99%

“…TCFL5 is a transcription factor of the bHLH family, first identified in spermatocytes. 43,44 Both MEK5c and Dusp6 can influence MAP kinase activity and interactions between the Notch and MAP kinase pathways have been described. 45 Our microarray results showed limited upregulation of MEK5c and TCFL5 transcripts after 24 h of culturing on S17-DL, while for unknown reasons RQ-PCR validation of TCFL5 showed high induction in this condition as well (Figure 3d).…”

Section: Novel Notch Target Genes In Hematopoietic Progenitorsmentioning

confidence: 99%

See 2 more Smart Citations

Identification of Notch target genes in uncommitted T-cell progenitors: no direct induction of a T-cell specific gene program

et al. 2006

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Novel Notch Target Genes In Hematopoietic Progenitorsmentioning

confidence: 99%

See 1 more Smart Citation

Identification of Notch target genes in uncommitted T-cell progenitors: no direct induction of a T-cell specific gene program

et al. 2006

View full text Add to dashboard Cite

“…Interestingly, a similar genomic clone named TCFL5 was isolated from testis (41). TCFL5 and Cha have identical C terminus sequences but differ in their N terminus.…”

Section: Cha and Usf-1 Interact In Vitro And In Vivo-mentioning

confidence: 99%

Cha, a Basic Helix-Loop-Helix Transcription Factor Involved in the Regulation of Upstream Stimulatory Factor Activity

Rodrı́guez

Gironès

Fresno

2003

Journal of Biological Chemistry

View full text Add to dashboard Cite

We report here the characterization of Cha, a transcription factor of the basic helix-loop-helix (bHLH) family. The basic region of Cha shares DNA-interacting amino acids with members of class C bHLH transcription factors. In addition, the HLH region of Cha presents a Myc-type dimerization domain signature required for heterodimer formation between members of this class. Cha protein and mRNA were ubiquitously expressed in many human tissues. Electrophoretic mobility shift assays showed that Cha and upstream stimulatory factor (USF)-1 formed a complex that specifically bound to Ebox DNA elements. Moreover, pull-down and co-immunoprecipitation experiments showed an interaction between Cha and USF-1. Cha did not bind to E-box DNA elements and required USF-1 for protein-DNA complex formation. Moreover, Cha inhibited USF-1-stimulated transcription of CD2 (a USF-1-dependent gene) and Ebox promoter reporter plasmids. Chromatin immunoprecipitation assays showed that Cha occupied the CD2 promoter in resting, but not in mitogen-stimulated, T cells. Finally, Cha mRNA and protein expression were high in resting T cells and absent in mitogen-activated T cells and inversely correlated with CD2 expression. Contrarily, overexpression of Cha in T cells significantly reduced CD2 expression. In summary, our results indicated that Cha is a new bHLH transcription factor that negatively regulates USF-dependent transcription.

show abstract

“…Tex11 regulates the level of meiotic crossovers and thus influences homologous chromosome segregation during spermatogenesis [24]. Tcfl5 is a basic helix-loop-helix transcription factor and interacts with the Calmegin gene promoter in spermatogenesis [25,26]. TEX14 localizes to germ cell intercellular bridges and is vital for intercellular bridges in male mice [27].…”

Section: Isolation Of Target Genes Of Hrbmy Protein Using the Snaap Tmentioning

confidence: 99%

Identifying mRNAs Bound by Human RBMY Protein in the Testis

Zeng

Liang

Zhao

et al. 2011

Journal of Reproduction and Development

View full text Add to dashboard Cite

Abstract. Rbmy gene encodes a germ-cell specific nuclear RNA-binding protein and is involved in spermatogenesis. To further investigate the specific events of spermatogenesis in which Rbmy plays a role, the target mRNAs of human RBMY protein were isolated and identified. Through the isolating specific nucleic acids associated with proteins (SNAAP) technique, we isolated twenty potential target genes of human RBMY protein from the human testis in the present study. Some of these target genes play important roles during spermatogenesis and have alternative transcripts in the testis. In this study, we focused on the human-related (never in mitosis gene a) kinase 10 (Nek10) gene, which belongs to the Nek protein kinase subfamily. Nek10 has two transcripts, and the results of RT-PCR and Electrophoretic Mobility Shift Assays (EMSA) show that hRBMY protein can only bind to transcript variant 2 of Nek10 and that hRbmy may take part in alternative splicing of Nek10. Isolation and identification of target genes of hRBMY will be helpful to further investigate the biological function of RBMY in spermatogenesis. Key words: Nek10, Rbmy gene, RNA-binding protein, SNAAP technique, Spermatogenesis (J. Reprod. Dev. 57: [107][108][109][110][111][112] 2011) permatogenesis is a complex cell differentiation process that requires highly regulated expression of lots of genes [1][2][3]. Many genes are mainly or exclusively expressed in the testis, and in most situations, further research has shown that they are only expressed in the germline cells [4]. Microdeletions at a specific region called the AZF in the long arm of Y chromosome have been described in significant proportions of oligo and azoospermic patients, suggesting that some genes located at these regions play an essential role during spermatogenesis [5]. Of all the genes located at the AZF region, Rbmy was the first of these candidate genes to be identified [6].There are many Rbm genes distributed throughout the Y chromosome, but only the genes in deletion interval AZFb give rise to detectable levels of protein [7]. According to the complete sequence of the human Y chromosome, it is now known that there are six Rbmy copies mapped in the AZFb region, but only one copy is actively transcribed [8]. Human and mouse Rbmy genes have a homologue on the human X chromosome (Rbmx) that maintains a widespread function, while Rbmy evolves a male-specific function in spermatogenesis [9]. There is another retrogene called hnRNPG-T that belongs to the same family as Rbmy and Rbmx, which is also specifically expressed in the testis. Previous study has shown that haploinsufficiency of hnRNPG-T results in abnormal sperm production in the mouse. Genetic defects resulting in reduced levels of hnRNPG-T may be a cause of male infertility in humans [10]. Although Rbmy is evolutionarily conserved in the human, mouse and marsupial [11][12][13], their expression patterns are not completely identical. The expression of mouse Rbmy is limited to spermatogonia and early spermatocytes, while human Rbmy ...

show abstract

Cloning of TCFL5 encoding a novel human basic helix-loop-helix motif protein that is specifically expressed in primary spermatocytes at the pachytene stage

Cited by 27 publications

References 16 publications

Identification of Notch target genes in uncommitted T-cell progenitors: no direct induction of a T-cell specific gene program

Identification of Notch target genes in uncommitted T-cell progenitors: no direct induction of a T-cell specific gene program

Cha, a Basic Helix-Loop-Helix Transcription Factor Involved in the Regulation of Upstream Stimulatory Factor Activity

Identifying mRNAs Bound by Human RBMY Protein in the Testis

Contact Info

Product

Resources

About