Cloning of TC-1 (C8orf4), a Novel Gene Found to Be Overexpressed in Thyroid Cancer

Chua, Elizabeth L.; Young, L. S.; Wu, Wan Man; Turtle, John R.; Dong, Qihan

doi:10.1006/geno.2000.6348

Cited by 56 publications

(82 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…KLF4 and ID1 are transcription factors that have been well studied in mice and are involved in development, neurogenesis, and angiogenesis and are specific to the endothelium (Yet et al 1998;Lyden et al 1999). The function of DOC1 and TC1 is unknown, and only limited information on their gene structures and their tissue expression is available (Mok et al 1994;Chua et al 2000). The DOC1 gene has some similarities to the heavy myosin chain of the mouse gene, suggesting that it might be involved in the organization of the cytoskeleton.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Early Genetic Mechanisms Underlying the Inhibitory Effects of Endostatin and Fumagillin on Human Endothelial Cells

et al. 2004

View full text Add to dashboard Cite

A tumor needs to initiate angiogenesis in order to develop its own blood supply, to grow, to invade, and to spread. Angiogenesis, under normal conditions, is a tightly regulated balance between endogenous pro- and antiangiogenic factors. In this study, we investigated, by microarray analysis, the effects of two known antiangiogenic agents (endostatin and fumagillin) on the gene expression profiles of human umbilical vein endothelial cells (HUVEC) in order to elucidate pathways common to the effects of these agents. We observed a majority of gene expression changes within 1 and 2 h of treatment. The genes demonstrating these early expression changes are involved in cell proliferation, gene transcription, and a number have unknown functions. We selected four genes (DOC1, KLF4, TC-1, ID1) from the microarray profile that showed a similar pattern of expression for both of the antiangiogenic agents we tested. We then used small interfering RNAs (siRNA) in an attempt to better understand the role of these selected genes in the inhibitory activity of these agents. Because the gene expression changes occurred within 1 and 2 h of treatment, these genes might be involved in the initial pathways of angiogenesis inhibition

show abstract

Section: Discussionmentioning

confidence: 99%

“…No information is available regarding sequence homology or protein function of the TC1 gene, except that it seems to be differentially expressed in thyroid tumors (Chua et al 2000).…”

Section: Discussionmentioning

confidence: 99%

Early Genetic Mechanisms Underlying the Inhibitory Effects of Endostatin and Fumagillin on Human Endothelial Cells

et al. 2004

View full text Add to dashboard Cite

show abstract

“…This approach has been successfully used for tissue-specific cDNA and genomic DNA subtraction (25,26) to isolate cancer-related and differentiation stage-specific genes (27)(28)(29)(30) and to clone drug-induced genes or pathogens from infected tissues (31)(32)(33). As compared with other low-yield subtractive hybridization techniques, the experimental approach used in this study is applicable to small amounts of rare samples, such as RNA extracted from islets derived from patients with recent onset of diabetes.…”

Section: Discussionmentioning

confidence: 99%

A Reg Family Protein Is Overexpressed in Islets From a Patient With New-Onset Type 1 Diabetes and Acts as T-Cell Autoantigen in NOD Mice

Gürr

Yavari

et al. 2002

Diabetes

View full text Add to dashboard Cite

Genes overexpressed in pancreatic islets of patients with new-onset type 1 diabetes are potential candidates for novel disease-related autoantigens. RT-PCR-based subtractive hybridization was used on islets from a patient who died at the onset of type 1 diabetes, and it identified a type 1 diabetes-related cDNA encoding hepatocarcinoma-intestine-pancreas/pancreatic-associated protein (HIP/PAP). This protein belongs to the family of Reg proteins implicated in islet regeneration; its gene contains a putative interleukin-6 (IL-6) response element. Islets from healthy cadaveric human donors released HIP/PAP protein into the culture medium, and this release was enhanced by the addition of IL-6. The expression pattern of mouse homologues of HIP/PAP was determined in pancreata of prediabetic and diabetic NOD mice. Both groups showed positive immunostaining for HIP/PAP in islets and ductal epithelium. To test whether HIP/PAP is a target of isletdirected autoimmunity, we measured splenic T-cell responses against HIP/PAP in NOD mice. Spontaneous proliferation was detected after 4 weeks. Lymphocytes from islet infiltrates and pancreatic lymph nodes from 7-to 10-week-old NOD mice were used to establish an HIP/PAP-specific I-A g7 -restricted T-cell line, termed WY1, that also responded to mouse islets. WY1 cells homed to islets of NOD-SCID mice and adoptively transferred disease when coinjected with purified CD8 ؉ cells from diabetic NOD mice. Our conclusion was that differential cloning of Reg from islets of a type 1 diabetic patient and the response of Reg to the cytokine IL-6 suggests that HIP/PAP becomes overexpressed in human diabetic islets because of the local inflammatory response. HIP/PAP acts as a T-cell autoantigen in NOD mice. Therefore, autoimmunity to HIP/PAP might create a vicious cycle, accelerating the immune process leading to diabetes. Diabetes 51:339 -346, 2002

show abstract

“…Chibby (Cby), a small (126 aa) protein antagonizes Wnt/ -catenin signaling by forming a ternary complex with protein 14-3-3 andcatenin [418,419]. The protein TC-1, associated with thyroid cancer, in turn can bind to Cby and inhibit its interactions withcatenin, leading to an upregulation of -catenin target genes [420,421]. Further tissue-specific proteins like Osterix (osteoblastsspecific transcription factor) are also able to repress the transcriptional complex through a disruption of Tcf binding to DNA [422].…”

Section: The -Catenin Tcf/lef Transcription Complexmentioning

confidence: 99%

Wnt/beta-Catenin Signaling and Small Molecule Inhibitors

Voronkov¹,

Krauß²

2012

CPD

View full text Add to dashboard Cite

Wnt/ -catenin signaling is a branch of a functional network that dates back to the first metazoans and it is involved in a broad range of biological systems including stem cells, embryonic development and adult organs. Deregulation of components involved in Wnt/ -catenin signaling has been implicated in a wide spectrum of diseases including a number of cancers and degenerative diseases. The key mediator of Wnt signaling, -catenin, serves several cellular functions. It functions in a dynamic mode at multiple cellular locations, including the plasma membrane, where -catenin contributes to the stabilization of intercellular adhesive complexes, the cytoplasm where -catenin levels are regulated and the nucleus where -catenin is involved in transcriptional regulation and chromatin interactions. Central effectors of -catenin levels are a family of cysteine-rich secreted glycoproteins, known as Wnt morphogens. Through the LRP5/6-Frizzled receptor complex, Wnts regulate the location and activity of the destruction complex and consequently intracellularcatenin levels. However, -catenin levels and their effects on transcriptional programs are also influenced by multiple other factors including hypoxia, inflammation, hepatocyte growth factor-mediated signaling, and the cell adhesion molecule E-cadherin. The broad implications of Wnt/ -catenin signaling in development, in the adult body and in disease render the pathway a prime target for pharmacological research and development. The intricate regulation of -catenin at its various locations provides alternative points for therapeutic interventions.

show abstract

Cloning of TC-1 (C8orf4), a Novel Gene Found to Be Overexpressed in Thyroid Cancer

Cited by 56 publications

References 13 publications

Early Genetic Mechanisms Underlying the Inhibitory Effects of Endostatin and Fumagillin on Human Endothelial Cells

Early Genetic Mechanisms Underlying the Inhibitory Effects of Endostatin and Fumagillin on Human Endothelial Cells

A Reg Family Protein Is Overexpressed in Islets From a Patient With New-Onset Type 1 Diabetes and Acts as T-Cell Autoantigen in NOD Mice

Wnt/beta-Catenin Signaling and Small Molecule Inhibitors

Contact Info

Product

Resources

About