Cloning of maltase gene from a methylotrophic yeast, Hansenula polymorpha

Liiv, Lele; Pärn, Pille; Alamäe, Tiina

doi:10.1016/s0378-1119(01)00359-6

Cited by 29 publications

(67 citation statements)

References 33 publications

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“…Cloning of the maltase gene (HpMAL1) of the above mentioned cluster showed that the promoter (P MAL1 ) of this gene was also functional in Escherichia coli, as high maltase activity in HpMAL1-possessing transformants was observed [3]. Since E. coli is the most popular host in recombinant protein production [4], P MAL1 may find application in biotechnology.…”

Section: Introductionmentioning

confidence: 99%

Hansenula polymorpha maltase gene promoter with sigma 70-like elements is feasible for Escherichia coli-based biotechnological applications: Expression of three genomic levansucrase genes of Pseudomonas syringae pv. tomato

Visnapuu

Mäe

Alamäe

2008

Process Biochemistry

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Section: Introductionmentioning

confidence: 99%

Hansenula polymorpha maltase gene promoter with sigma 70-like elements is feasible for Escherichia coli-based biotechnological applications: Expression of three genomic levansucrase genes of Pseudomonas syringae pv. tomato

Visnapuu

Mäe

Alamäe

2008

Process Biochemistry

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“…The hydropathy plot and SignalP analysis (http://www.cbs.dtu.dk/services/SignalP/) of MalT revealed the absence of the putative signal peptide sequence at its N-terminus, indicating that MalT would be an intracellular enzyme. A BLAST search showed MalT has the highest homology (55% identity and 73% similarity) to the yeast Candida albicans maltase (Geber et al, 1992), and high homology (43-49% identity and 63-66% similarity) to Hansenula (Liiv et al, 2001), Saccharomyces (Charron et al, 1986), and Kluyveromyces (CAB46746) a-glucosidases or maltases ( Supplementary Fig. 4).…”

Section: Gene Organization and Characterization Of Mal Cluster Genes mentioning

confidence: 99%

Characterization and expression analysis of a maltose-utilizing (MAL) cluster in Aspergillus oryzae

Hasegawa

Takizawa

Suyama

et al. 2010

Fungal Genetics and Biology

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“…The primers design for cloning of the gene encoding maltase in S. pombe was carried out according to the conserved sequence of maltase genes in Saccharomyces carlsbergensis, Hansenula polymorpha, Candida albicans and the complete sequence of genomic DNA in S. pombe [18][19][20][21].…”

Section: Assay Of Reducing Sugar In the Mediummentioning

confidence: 99%

Studies on inositol-mediated expression of MAL gene encoding maltase and phospholipid biosynthesis in Schizosaccharomyces pombe

Shen

Chi

2006

J IND MICROBIOL BIOTECHNOL

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In this study, the effects of inositol addition on expression of the MAL gene encoding maltase and phosphatidylinositol (PI) biosynthesis in Schizosaccharomyces pombe (a naturally inositol-requiring strain) were examined. We found that specific maltase activity was at its maximum when the concentration of added inositol reached 6 microg ml(-1) in a synthetic medium containing 2.0% (w/v) glucose. When the concentration of added inositol was 1 microg ml(-1) in the medium, repression of MAL gene expression occurred at glucose concentration higher than 0.2% (w/v). However, when S. pombe was cultured in the synthetic medium containing 6 microg ml(-1), repression of maltase gene expression occurred only at initial glucose concentration above 1.0% (w/v). More mRNA encoding maltase was detected in the cells grown in the medium with 6 microg ml(-1) inositol than in those grown in the same medium with 1 microg ml(-1) inositol. These results demonstrate that higher inositol concentrations in the synthetic medium could derepress MAL gene expression in S. pombe. PI content of the yeast cells grown in the synthetic medium with 6 microg ml(-1) of inositol was higher than that of the yeast cells grown in the same medium with 1 microg ml(-1) of inositol. This means that PI may be involved in the derepression of MAL gene expression in S. pombe.

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Cloning of maltase gene from a methylotrophic yeast, Hansenula polymorpha

Cited by 29 publications

References 33 publications

Hansenula polymorpha maltase gene promoter with sigma 70-like elements is feasible for Escherichia coli-based biotechnological applications: Expression of three genomic levansucrase genes of Pseudomonas syringae pv. tomato

Hansenula polymorpha maltase gene promoter with sigma 70-like elements is feasible for Escherichia coli-based biotechnological applications: Expression of three genomic levansucrase genes of Pseudomonas syringae pv. tomato

Characterization and expression analysis of a maltose-utilizing (MAL) cluster in Aspergillus oryzae

Studies on inositol-mediated expression of MAL gene encoding maltase and phospholipid biosynthesis in Schizosaccharomyces pombe

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