Cloning of Lactobacillus plantarum IAM 12477 lysine biosynthetic genes encoding functional aspartate semialdehyde dehydrogenase, dihydrodipicolinate synthase, and dihydrodipicolinate reductase

Cahyanto, Muhammad Nur; Kawasaki, Hiroko; Fujiyama, Kazuhito; Seki, Tatsuji

doi:10.1007/s11274-005-9048-6

Cited by 5 publications

(6 citation statements)

References 26 publications

(20 reference statements)

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“…Of these microbes, lactobacilli were reported as a group of colonizers in the human small intestinal mucosa with symbiotic relationships with human body. Many clinical studies have reported that probiotics, such as L. plantarum, have beneficial health effects on human immune system (Cahyanto et al 2006;Reid et al 2003). And it has been proved that most members of the group exert additional probiotic properties, providing health benefits to the host via regulation of immune system functions.…”

Section: Discussionmentioning

confidence: 99%

Identification of DC-SIGN as the receptor during the interaction of Lactobacillus plantarum CGMCC 1258 and dendritic cells

Liu

Shen

et al. 2010

World J Microbiol Biotechnol

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Lactobacillus plantarum can exert additional probiotic effects via regulation of human immune system. However, the direct interaction between probiotics and the receptors of immune cells still needs to be further studied.To identify the receptor of dendritic cells during the interaction with L. plantarum. Dendritic cells were pretreated with L. plantarum and the antibody to dendritic cells specific intercellular adhesion molecule-grabbing nonintegrin (DC-SIGN), toll like receptor (TLR)-2 and TLR-4. The maturation of immature dendritic cells, cytokine production, and modulation of T cells were studied by flow cytometry. Adherence between L. plantarum and dendritic cells were studied by ELISA, flow cytometry, and Western blot. L. plantarum could mature dendritic cells by up-regulating MHC-II and CD80 and CD86. Anti-inflammatory interlectin (IL)-10 and IL-6 was up-regulated and pro-inflammatory IL-12p70 was retro-regulated by L. plantarum. L. plantarum may interact with DC-SIGN and modulate of T to differentiate into IL-4 producing T cells. The interaction of L. plantarum and DC-SIGN and the biological effects could be blocked by EDTA and antibody to DC-SIGN. Effects of L. plantarum were concentrationdependent. L. plantarum could bind to DC-SIGN to improve DC maturation at different ratios, regulate the secretion of anti-inflammatory and pro-inflammatory cytokines, and induce the polarization of interlectin-4-producing T cells.

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Section: Discussionmentioning

confidence: 99%

Identification of DC-SIGN as the receptor during the interaction of Lactobacillus plantarum CGMCC 1258 and dendritic cells

Liu

Shen

et al. 2010

World J Microbiol Biotechnol

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“…Interestingly, the genome of A. tumefaciens has been sequenced and reveals 10 putative dapA genes that potentially encode DHDPS. Besides rare reports of two dapA genes in Bacillus anthracis and Lactobacillus plantarum and three genes in Hahella chejuensis and Halobacillus halophilus , most bacterial species have a single dapA gene. Therefore, Agrobacterium tumefaciens is the only bacterial species whose genome has been sequenced to date to harbor more than three putative dapA genes.…”

Section: Introductionmentioning

confidence: 99%

Identification of thebona fideDHDPS from a common plant pathogen

et al. 2014

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Agrobacterium tumefaciens is a Gram-negative soil-borne bacterium that causes Crown Gall disease in many economically important crops. The absence of a suitable chemical treatment means there is a need to discover new anti-Crown Gall agents and also characterize bona fide drug targets. One such target is dihydrodipicolinate synthase (DHDPS), a homo-tetrameric enzyme that catalyzes the committed step in the metabolic pathway yielding meso-diaminopimelate and lysine. Interestingly, there are 10 putative DHDPS genes annotated in the A. tumefaciens genome, including three whose structures have recently been determined (PDB IDs: 3B4U, 2HMC, and 2R8W). However, we show using quantitative enzyme kinetic assays that nine of the 10 dapA gene products, including 3B4U, 2HMC, and 2R8W, lack DHDPS function in vitro. A sequence alignment showed that the product of the dapA7 gene contains all of the conserved residues known to be important for DHDPS catalysis and allostery. This gene was cloned and the recombinant product expressed and purified. Our studies show that the purified enzyme (i) possesses DHDPS enzyme activity, (ii) is allosterically inhibited by lysine, and (iii) adopts the canonical homo-tetrameric structure in both solution and the crystal state. This study describes for the first time the structure, function and allostery of the bona fide DHDPS from A. tumefaciens, which offers insight into the rational design of pesticide agents for combating Crown Gall disease.

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“…The dapA gene encodes the rate-limiting enzyme for the first specific step of lysine biosynthesis (Hutton et al 2003;Cahyanto et al 2006). Decreased free lysine in the dapA -mutant confirmed modified amino acid metabolism as a result of removing the dapA gene, which partly contributed to increase COR synthesis.…”

Section: Discussionmentioning

confidence: 89%

“…The first specific step in lysine biosynthesis is conversion of pyruvate and aspartate semi-aldehyde to dihydrodipicolinate, which is catalysed by dihydrodipicolinate synthase (Hutton et al 2003;Cahyanto et al 2006). Dihydrodipicolinate synthase is the rate-limiting enzyme for lysine biosynthesis and is encoded by the dapA gene.…”

Section: Introductionmentioning

confidence: 99%

Effects of dapA gene deletion on coronatine biosynthesis in Pseudomonas syringae pv. glycinea PG4180

Zhang

Liu

et al. 2010

World J Microbiol Biotechnol

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Coronatine (COR) is a structural and functional analogue of jasmonic acid that might be employed in agriculture to elicit plant resistance against various aggressors. However, the yield of COR is low both in chemosynthesis and biosynthesis, so broad investigation of COR is difficult. Coronatine combines two distinct components: coronafacic acid (CFA) and coronamic acid (CMA). Synthesis of both CMA and CFA is involved in Lisoleucine metabolism, so the objective of this work was to investigate if COR production can be improved by regulating amino acid biosynthesis in P. syringae pv. glycinea. Inhibition of dihydrodipicolinate synthase was achieved by removing the dapA gene via homologous recombination, which resulted in a COR yield by the dapA -mutant of about 1.5-fold greater than the wild strain. Thus, regulation of amino acid metabolism is a feasible way to increase COR production, which could be a more effective method than adding substrates into culture medium.

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Cloning of Lactobacillus plantarum IAM 12477 lysine biosynthetic genes encoding functional aspartate semialdehyde dehydrogenase, dihydrodipicolinate synthase, and dihydrodipicolinate reductase

Cited by 5 publications

References 26 publications

Identification of DC-SIGN as the receptor during the interaction of Lactobacillus plantarum CGMCC 1258 and dendritic cells

Identification of DC-SIGN as the receptor during the interaction of Lactobacillus plantarum CGMCC 1258 and dendritic cells

Identification of thebona fideDHDPS from a common plant pathogen

Effects of dapA gene deletion on coronatine biosynthesis in Pseudomonas syringae pv. glycinea PG4180

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